Hi Andrew,

Merging the data prior to upload would probably be simplest. Files in a galaxy history are not in .tar format at this time.


Loading forward and reverse separately will most likely be important from a scientific perspective for analysis.

Once ready for upload, you can tar or gz - as long as each load is a single file - or leave uncompressed - either is fine. Using FTP is required for larger data (>= 2G) and using a client that will allow you to track progress/resume an interrupted load can be helpful. Each file can be up to 50G in size if you have an account.
http://wiki.galaxyproject.org/FTPUpload

Hopefully this helps,

Jen
Galaxy team

On 4/5/13 3:20 AM, Thompson, Andrew wrote:

Hi

I have received Illumina paired-end genome sequence data as a .tar file. When unpacked the data for each genome accession is split into about 100 fastq files. Total of about 37 Gpb per genome.

Can you recommend the best way to organise this data prior to mapping to reference genome?

I can concatenate unpacked files using DOS command line into forward and reverse before uploading: is this the best approach? Is there a tools that will start with the .tar file?

Andrew



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