[ccp4bb] Coot on Mac OSX Monterey

[Laurence Pearl]

Does anyone know where I can download a pre-built Coot that will work on OS X 
Monterey  (12.2) on an iMac with an Intel i7 ?

The packages I can find all fail at the File finder which comes up blank. X11 
latest is installed.

Laurence


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
School of Life Sciences, University of Sussex
Brighton, BN1 9RQ, UK

+44-(0)1273 678349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin







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Re: [ccp4bb] me7G-pp- mRNA 5' cap dictionary

[Laurence Pearl]

Yes but without the methyl groups on the sugars. I scavenged a GTA capped RNA 
from a PDB - 5wfh - which draws the link from the GTA to the 5’-phosphate of 
the RNA, but when you regularise them in coot, it breaks the connecting bond.

However after just starting to reply to you I realised that the GTA is marked 
as HETATM, so I just edited it to ATOM in the PDB and the wonderful coot 
minimiser now bonds everything perfectly.

Thanks all who responded.


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
School of Life Sciences, University of Sussex
Brighton, BN1 9RQ, UK

+44-(0)1273 678349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin





On 17 May 2021, at 17:13, Paul Emsley 
mailto:pems...@mrc-lmb.cam.ac.uk>> wrote:

On 17/05/2021 17:08, Laurence Pearl wrote:
Can anyone tell me where to find a model dictionary entry for COOT and/or 
phenix for the 7meG-pp residue that forms the 5’ cap on a messenger RNA ? I 
need to be able to bond it to the 5’-end of an RNA model and bond it to the 
existing 5’-phosphate of the first ribonucleotide.

Do you mean the orange/yellow entity here?


Paul





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[ccp4bb] me7G-pp- mRNA 5' cap dictionary

[Laurence Pearl]

Can anyone tell me where to find a model dictionary entry for COOT and/or 
phenix for the 7meG-pp residue that forms the 5’ cap on a messenger RNA ? I 
need to be able to bond it to the 5’-end of an RNA model and bond it to the 
existing 5’-phosphate of the first ribonucleotide.

Thanks,

Laurence


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
School of Life Sciences, University of Sussex
Brighton, BN1 9RQ, UK

+44-(0)1273 678349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin








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[ccp4bb] Prof. Mavis Agbandje-McKenna

[Laurence Pearl]

Very sad to hear of the untimely death of Prof. Mavis Agbandje-McKenna who I 
had the honour and pleasure of working alongside in Stephen Neidle’s lab at The 
Institute of Cancer Research in the late 1980s - there is an obituary from 
University of Florida here : 
https://m.ufhealth.org/news/2021/world-renowned-uf-health-biochemistry-researcher-mavis-agbandje-mckenna-phd-dies


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
University of Sussex, Brighton, BN1 9RQ, UK

Phone +44-(0)1273  678 349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin





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[ccp4bb] Main chain from Ca coordinates

[Laurence Pearl]

Can anyone point me at a good server/program for generating polypeptide main 
chain from Calpha coordinates ? Sadly the EMBL-EBI MaxSprout server seems to 
have been retired.


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
University of Sussex, Brighton, BN1 9RQ, UK

Phone +44-(0)1273  678 349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin





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[ccp4bb] Postdoc in DNA Repair Structural Biology

[Laurence Pearl]

A Post-doctoral position funded by Cancer Research UK is available from 1st 
October 2019 in the laboratory of Prof. Laurence Pearl FRS and Dr. Antony 
Oliver, in the Genome Damage and Stability Centre at the University of Sussex, 
to study the structure and mechanism of multi-protein complexes involved in the 
recognition and repair of DNA single-strand breaks and gaps, primarily using 
single particle reconstruction by cryo-electron microscopy.

https://www.sussex.ac.uk/about/jobs/post-doctoral-research-fellow-structural-biology


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
University of Sussex, Brighton, BN1 9RQ, UK

Phone +44-(0)1273  678 349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin






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[ccp4bb] Postdoc in Pearl/Oliver lab at Genome Damage and Stability Centre, Sussex

[Laurence Pearl]

Post-Doctoral Research Fellow : Structural Biology of DNA Single-strand Break 
Repair
(Ref 1739)


A Post-doctoral position funded by Cancer Research UK is available from 1st 
October 2019 in the laboratory of Prof. Laurence Pearl FRS and Dr. Antony 
Oliver in the Genome Damage and Stability Centre at the University of Sussex, 
to study the structure and mechanism of multi-protein complexes involved in the 
recognition and repair of DNA single-strand breaks and gaps, primarily using 
single particle reconstruction by cryo-electron microscopy.

Based in the School of Life Sciences, the Genome Damage and Stability Centre, 
is an internationally renowned Institute carrying out research on the response 
of cells to DNA damage, genome instability and its relationship to disease.

Recent work from the Pearl and Oliver groups and an overview of research in the 
Genome Damage and Stability Centre can be found at 
http://www.sussex.ac.uk/gdsc/researchgroups

The School of Life Sciences is committed to equality and valuing diversity, and 
currently holds an Athena SWAN Silver Award. Applications are particularly 
welcomed from women and black and minority ethnic candidates, who are 
under-represented in academic posts in Science, Technology, Engineering, 
Medicine and Mathematics (STEMM) at Sussex. The School of Life Sciences 
welcomes applications to academic posts from candidates who wish to work 
part-time or as job-sharers.

The University offers various schemes to provide real benefits to parents, 
these can be found at Family Friendly 
Policies<http://www.sussex.ac.uk/humanresources/personnel/familyfriendlypolicies>

Potential candidates are strongly encouraged to make informal contact with 
Prof. Pearl laurence.pe...@sussex.ac.uk<mailto:laurence.pe...@sussex.ac.uk> or 
Dr. Oliver antony.oli...@sussex.ac.uk<mailto:antony.oli...@sussex.ac.uk> before 
applying.

   The University of Sussex values the diversity of its staff and students and 
we welcome applicants from all backgrounds

Download job description and person specification Ref 1739 [PDF 
81.79KB]<https://www.sussex.ac.uk/about/documents/1739-fps.pdf>


How to apply

Download our academic post application form [DOC 
301.50KB]<https://www.sussex.ac.uk/humanresources/documents/acadnw.doc> and 
personal details and equal opportunities form [DOC 
162.50KB]<https://www.sussex.ac.uk/humanresources/documents/personal-data-equal-opps-form-may-2018.doc>
 and fill in all sections.

Applications should be accompanied by a full CV, a statement of research 
interests and aspirations (not more than 4 pages), and the names of three 
academic referees.

Email your completed application,CV, statement of research interests and 
aspirations, and personal details and equal opportunities form, to 
lifescirecruitm...@sussex.ac.uk<mailto:lifescirecruitm...@sussex.ac.uk>

You should attach your application form and all documents to the email (don't 
use a web-based upload/weblink service) and use the format job reference number 
/ job title / your name in the subject line.

You can also send your application by post to Human Resources Division, Sussex 
House, University of Sussex, Falmer, Brighton, BN1 9RH.

Download our terms and conditions summary for Research Faculty Terms and 
Conditions [DOC 
36.00KB]<https://www.sussex.ac.uk/humanresources/documents/research-summ>


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
University of Sussex, Brighton, BN1 9RQ, UK

Phone +44-(0)1273 678 349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin








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[ccp4bb] Team Leader and Fellowship Opportunities at Institute of Cancer Research

[Laurence Pearl]

The Institute of Cancer Research, London – Division of Structural Biology
Team Leader Positions & Independent Fellowship opportunities
The ICR is committed to attracting and developing the best minds in the world 
to join us in our mission – to make the discoveries that defeat cancer
The Institute of Cancer Research, London, is one of the world’s most 
influential cancer research organisations, with an outstanding record of 
achievement dating back more than 100 years. We provided the first convincing 
evidence that DNA damage is the basic cause of cancer, laying the foundation 
for the now universally accepted idea that cancer is a genetic disease. Today, 
we are world leaders in discovering new-targeted cancer drugs, identifying new 
cancer genes and developing new forms of precision radiotherapy.
Under the leadership of our Chief Executive and President, Professor Paul 
Workman FRS, The Institute of Cancer Research (ICR) is ranked as the UK’s 
leading higher education institution for the quality and impact of its 
research. Together with our partner The Royal Marsden NHS Foundation Trust, we 
are rated in the top five centres for cancer research and treatment worldwide. 
The ICR is committed to attracting and developing the best minds in the world 
to join us in our mission – to make the discoveries that defeat cancer.
Team Leader positions
As part of a programme of expansion of the Division of Structural Biology, we 
wish to establish three new research teams at the Institute’s Chester Beatty 
Laboratories in Chelsea, Central London. We are interested in established 
investigators, for whom permanent Faculty positions are available, and in 
emerging researchers able to attract externally-funded Personal Fellowships, 
for whom we will provide mentoring, and a generous support package and 
transparent review process.
Reflecting the Institute’s mission, we are looking for researchers whose 
interests inform our understanding of the origins, development and treatment of 
cancer. Current areas of interest include signal transduction, genome 
stability, transcription regulation, RNA processing, molecular chaperones, 
targeted protein degradation, and structure-based drug design.
The Division is exceptionally well equipped for modern structural biology, with 
excellent managed facilities for protein production, biophysical analysis, 
crystallisation and X-ray diffraction, and computation, as well as Techni T12 
and F20 cryo-electron microscopes, and a state-of the-art FEI Glacios/Falcon 3. 
The Division also has a 30% share of a Krios/K3 cryoEM facility at the Crick 
Institute, and rolling allocations of cryoEM time at the eBIC national 
facility, and X-ray diffraction time at the Diamond, ESRF and Soleil 
synchrotrons.
To apply online for Team Leader positions, please click on this 
link<https://icr.tal.net/vx/lang-en-GB/mobile-0/appcentre-ext/brand-4/candidate/jobboard/vacancy/1/adv/>.
 This will re-route you to the ICR’s Web Recruitment System, where you will 
need to register for an account (if you have not already) and log in. Along 
with completing the online application form, you will be asked to attach the 
following documents and failure to do so will mean your application cannot be 
considered on this occasion:
•   Full CV
•   Lists of major publications, achievements, research grants, 
distinctions.
•   Covering letter including the names and contact details of three 
academic referees
•   Research plan (five to six pages outlining your current research 
interests and research programme for the next 5 years)
For informal enquiries, please contact the Head of Division, Professor Laurence 
Pearl FRS – laurence.pe...@icr.ac.uk<mailto:laurence.pe...@icr.ac.uk>
The Closing date for Team Leader applications is15th July 2019
Fellowship positions
The ICR is proactively seeking candidates who can be supported in making 
applications for personal fellowships provided by key fellowship schemes across 
the UK such as CRUK, Wellcome, MRC, etc.  ICR’s Research Operations Team will 
support the candidates in drafting applications to the major funding bodies. 
The salary component will be based on ICR’s own CDF scale. ICR will offer an 
additional support package to researchers with personal fellowships of a PhD 
studentship and a 3-year post-doctoral position with consumables paid for from 
ICR General Funds.
For informal enquiries, please contact the Head of Division, Professor Laurence 
Pearl FRS – laurence.pe...@icr.ac.uk<mailto:laurence.pe...@icr.ac.uk>
We are particularly keen to hear from women and members of minority ethnic 
groups who are currently under-represented in these positions








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[ccp4bb] Postdoc in Pearl lab 2

[Laurence Pearl]

Post-Doctoral Research Fellow: Conformational Dynamics of HSP90-Kinase Complexes


A Wellcome Trust-funded Post-doctoral Research Fellow position is available 
from 1st October 2018 in the laboratory of Prof. Laurence Pearl FRS and Dr. 
Chris Prodromou, to study the recruitment, activation and targeted degradation 
of protein kinases that depend on the HSP90 molecular chaperone system.

The successful applicant will analyse the conformational dynamics of protein 
kinase/chaperone complexes, using a range of complimentary structural biology 
techniques including NMR, X-ray crystallography, fluorescence spectroscopy and 
cryoEM.

http://m.sussex.ac.uk/about/jobs/post-doctoral-research-fellow-3342


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology
Genome Damage and Stability Centre
University of Sussex, Brighton, BN1 9RQ, UK

Phone +44-(0)1273  678 349

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin





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Re: [ccp4bb] CCP4BB Digest - 26 Nov 2016 to 27 Nov 2016 - Special issue (#2016-322)

[Laurence Pearl]

Picture of electron density didn’t come through, but three planar fused rings 
sounds like a flavin - is your protein involved in redox reactions and is it 
coloured when concentrated ? The only four ring molecules that spring to mind 
are steroids, but these wouldn’t be all that flat and you would get a sense of 
the pucker of the rings at that resolution.


On 27 Nov 2016, at 10:34, CCP4BB automatic digest system 
> wrote:

There are 2 messages totaling 65823 lines in this issue.

Topics in this special issue:

 1. Problems finding the correct Cif file of a crystal structure
 2. Density for an unknown ligand

--

Date:Sun, 27 Nov 2016 10:09:48 +
From:Harry Powell >
Subject: Re: Problems finding the correct Cif file of a crystal structure

Hi

Cambridge Structural Database (CSD) should have only those small molecule 
structures that have at least one organic carbon - so it may have copper 
acetate (but which? There may be several...). For strictly inorganic structures 
you would need the Inorganic Crystal Structural Database (ICSD).

Harry
--
Dr Harry Powell
Chairman of International Union of Crystallography Commission on 
Crystallographic Computing
Chairman of European Crystallographic Association SIG9 (Crystallographic 
Computing)

On 26 Nov 2016, at 22:23, Eleanor Dodson 
> wrote:

There is a data bae - cambridge Structural Data Base with most crystal 
structures coordinates and measure data in it. if your university subscribes 
you should be able to acess it.
Eleanor dodson

On 26 November 2016 at 21:00, Bianca Valdes 
> wrote:
Hello!

I'm currently a PhD student. I'm taking a crystallography class and for our 
final project the professor wants us to compare the theoretical cif file of 
Copper Acetate with the experimental one we are generating. I found a cif file 
but is very incomplete,  do you guys now where I can find the complete and 
correct Cif file for Copper Acetate?


Thanks a lot!

Bianca


--

Date:Sun, 27 Nov 2016 18:33:34 +0800
From:Wei Liu >
Subject: Density for an unknown ligand

Dear all,

We have recently crystallized a recombinant protein produce from E. coli, and 
determined the structure at 1.9 Å. The asymmetric unit contains two protein 
monomers. Beyond our expectation, strong Fo - Fc density is present at a cleft 
of one subunit, but not in the other. Density maps are given in the snapshots 
attached to this email. We tried to model Tris or Bis-tris propane that were 
used as the purification and crystallization buffers, but apparently either of 
them poorly fitted in this density.  The molecule that can be modeled here 
seems more likely to be a ligand comprising 3 or 4 rings with good planarity, 
however we did not add any additives in our crystallization trials. So we think 
it should be something from E. coli, which has high affinity to our protein. I 
wonder if anybody can figure out which molecule well fits to the electron 
density.

Best wishes
Wei Liu




--

End of CCP4BB Digest - 26 Nov 2016 to 27 Nov 2016 - Special issue (#2016-322)
*


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544: PA +44-(0)1273 872 699

"Live Modestly and do Serious Things .. "
- Dorothy Crowfoot Hodgkin

[ccp4bb] Postdoc in Laurence Pearl's Lab

[Laurence Pearl]

Postdoctoral Research Fellow in Structural Biology of Hsp90 complexes (Fixed 
term) Ref 143

School of Life Sciences - Genome Damage and Stability Centre

Fixed term for 3 years, full time.
Salary range:  starting at £31,342 and rising to £37,394 per annum.
Closing date for applications: 31 May 2015

Description

The School of Life Sciences is at the forefront of research in the biological 
sciences in the UK, coming in the top 10 in the REF 2014.

A Wellcome Trust-funded postdoctoral position is available immediately in the 
laboratory of Professor Laurence Pearl FRS 
(http://www.sussex.ac.uk/lifesci/pearllab/) in the MRC Genome Damage and 
Stability Centre at Sussex University, to study the structural basis for client 
protein recruitment and activation by the Hsp90 molecular chaperone system.  We 
are very well equipped for all aspects of modern structural biology, with 
state-of-the-art laboratories for molecular biology, recombinant expression in 
bacterial and eukaryotic systems, biochemistry, biophysics and X-ray 
crystallography. Excellent synchrotron access (~2 days/month) is available 
through rolling beam allocation programmes at Diamond and ESRF.

Applicants must have a PhD, and experience in recombinant expression and 
protein purification. Experience of crystallisation and X-ray crystallography 
and/or single particle electron microscopy would be an advantage. Information 
on our previous work in this field can be found at by searching PubMed (pearl 
[au] and hsp90).

The postholder will be responsible for expression, purification, 
crystallization and structural analysis of protein complexes involving Hsp90, 
co-chaperones and client proteins that depend on Hsp90 for their biological 
function.

Informal enquiries can be made to Professor Pearl 
laurence.pe...@sussex.ac.ukmailto:laurence.pe...@sussex.ac.uk.

The School is committed to equality and valuing diversity, and currently holds 
an Athena SWAN Bronze Award. Applications are particularly welcomed from women 
and black and minority ethnic candidates, who are under-represented in academic 
posts in science and engineering at Sussex. The School of Life Sciences 
welcomes applications to academic posts from candidates who wish to work 
part-time or as job-sharers.

The University offers various schemes to provide real benefits to parents, 
these can be found 
athttp://www.sussex.ac.uk/humanresources/personnel/familyfriendlypolicies

Ref 143 Further particulars including person specification [PDF 
134.84KB]http://www.sussex.ac.uk/aboutus/documents/143-fps.pdf

Essential information

Terms and conditions

Research Faculty Terms and Conditions SUMMARY [DOC 
36.00KB]http://www.sussex.ac.uk/humanresources/documents/research-summ

Application

Application form - academic post [DOC 
301.50KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.doc

Please send completed application form by email to: 
lifescirecruitm...@sussex.ac.ukmailto:lifescirecruitm...@sussex.ac.uk

When emailing your application please use the following format in the 'subject' 
line: (post reference number / post title / your name). If you are applying for 
more than one post advertised by the University, please send a separate email 
and application form for each post. Please attach your completed application 
form and any other documents directly to the email rather than using a 
web-based upload / weblink service (e.g. SkyDrive) otherwise we may not receive 
your application due to incompatibility with our email software.

Or post to: Human Resources Division, Sussex House, University of Sussex, 
Falmer, Brighton, BN1 9RH.

Applications should be received by midnight on the closing date.


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544: PA +44-(0)1273 872 699

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

[ccp4bb] Postdoc position on Hsp90 Structural Biology

[Laurence Pearl]

Postdoctoral Research Fellow in Structural Biology of Hsp90 complexes (Fixed 
term) Ref 143

http://www.sussex.ac.uk/aboutus/jobs/143


School of Life Sciences - Genome Damage and Stability Centre

Fixed term for 3 years, full time.
Salary range:  starting at £31,342 and rising to £37,394 per annum.
Closing date for applications: 31 May 2015

Description

The School of Life Sciences is at the forefront of research in the biological 
sciences in the UK, coming in the top 10 in the REF 2014.

A Wellcome Trust-funded postdoctoral position is available immediately in the 
laboratory of Professor Laurence Pearl FRS 
(http://www.sussex.ac.uk/lifesci/pearllab/) in the Genome Damage and Stability 
Centre at Sussex University, to study the structural basis for client protein 
recruitment and activation by the Hsp90 molecular chaperone system.  We are 
very well equipped for all aspects of modern structural biology, with 
state-of-the-art laboratories for molecular biology, recombinant expression in 
bacterial and eukaryotic systems, biochemistry, biophysics and X-ray 
crystallography. Excellent synchrotron access (~2 days/month) is available 
through rolling beam allocation programmes at Diamond and ESRF.

Applicants must have a PhD, and experience in recombinant expression and 
protein purification. Experience of crystallisation and X-ray crystallography 
and/or single particle EM, would be an advantage. Information on our previous 
work in this field can be found by searching PubMed (pearl [au] and hsp90).

The postholder will be responsible for expression, purification, 
crystallization and structural analysis of protein complexes involving Hsp90, 
co-chaperones and client proteins that depend on Hsp90 for their biological 
function.

Informal enquiries can be made to Professor Pearl 
laurence.pe...@sussex.ac.ukmailto:laurence.pe...@sussex.ac.uk.

The School is committed to equality and valuing diversity, and currently holds 
an Athena SWAN Bronze Award. Applications are particularly welcomed from women 
and black and minority ethnic candidates, who are under-represented in academic 
posts in science and engineering at Sussex. The School of Life Sciences 
welcomes applications to academic posts from candidates who wish to work 
part-time or as job-sharers.

The University offers various schemes to provide real benefits to parents, 
these can be found at 
http://www.sussex.ac.uk/humanresources/personnel/familyfriendlypolicies



Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544: PA +44-(0)1273 872 699

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

Re: [ccp4bb] השב: [ccp4bb] Why nobody comments about the Nobel committee decision?

[Laurence Pearl]

Also good to remember the contributions of Peter Kollman who also died too 
young and would certainly have been a contender in this splendidly well 
directed Nobel prize


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544: PA +44-(0)1273 872 699

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

[ccp4bb] Postdoc in Pearl Lab on Hsp90 Complexes

[Laurence Pearl]

Postdoctoral Research Fellow in Structural Biology of Hsp90 Complexes (Fixed 
term) Ref 297

School of Life Sciences - Genome Damage and Stability Centre

Full time, fixed term for 36 months
Salary range: starting at £30,424 and rising to £36,298 per annum.
Closing date for applications: 27 September 2013x-apple-data-detectors://2

Description

A Wellcome Trust-funded postdoctoral position is available immediately in the 
laboratory of Professor Laurence Pearl FRS 
http://www.sussex.ac.uk/lifesci/pearllab/  in the MRC Genome Damage and 
Stability Centre at Sussex University, to study the structural basis for client 
protein recruitment and activation by the Hsp90 molecular chaperone system.  We 
are very well equipped for all aspects of modern structural biology, with 
state-of-the-art laboratories for molecular biology, recombinant expression in 
bacterial and eukaryotic systems, biochemistry, biophysics and X-ray 
crystallography. Excellent synchrotron access (~2 days/month) is available 
through rolling beam allocation programmes at Diamond and ESRF.

Applicants must have a PhD, and experience in recombinant expression and 
protein purification. Experience of crystallisation and X-ray crystallography 
would be an advantage. Information on our previous work in this field can be 
found at by searching PubMed (pearl [au] and hsp90).

The postholder will be responsible for expression, purification, 
crystallization and structural analysis of protein complexes involving Hsp90, 
co-chaperones and client proteins that depend on Hsp90 for their biological 
function.

Informal enquiries can be made to Professor 
pearllaurence.pe...@sussex.ac.ukmailto:laurence.pe...@sussex.ac.uk.

Ref 297 Further particulars including person specification [PDF 
78.54KB]http://www.sussex.ac.uk/aboutus/documents/297-fps.pdf

Essential information
Terms and conditions

Research Faculty Terms and Conditions SUMMARY [DOC 
36.00KB]http://www.sussex.ac.uk/humanresources/documents/research-summ

Application

Application form - academic post [DOC 
301.50KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.doc
Application form - academic post [PDF 
57.04KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.pdf

Please send completed application form by email 
to:lifescirecruitm...@sussex.ac.ukmailto:lifescirecruitm...@sussex.ac.uk

When emailing your application please use the following format in the 'subject' 
line: (post reference number / post title / your name). If you are applying for 
more than one post advertised by the University, please send a separate email 
and application form for each post. Please attach your completed application 
form and any other documents directly to the email rather than using a 
web-based upload / weblink service (e.g. SkyDrive) otherwise we may not receive 
your application due to incompatibility with our email software.

Or post to: Human Resources Division, Sussex House, University of Sussex, 
Falmer, Brighton, BN1 9RH.

Applications should be received by midnight on the closing date.


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544tel:+44-(0)1273%20876%20544: PA+44-(0)1273 872 
699tel:+44-(0)1273%20872%20699

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

[ccp4bb] Postdoc in Pearl Lab on Hsp90 Complexes - correction

[Laurence Pearl]

Postdoctoral Research Fellow in Structural Biology of Hsp90 Complexes (Fixed 
term) Ref 297

School of Life Sciences - Genome Damage and Stability Centre

Full time, fixed term for 36 months
Salary range: starting at £30,424 and rising to £36,298 per annum.
Closing date for applications: 27 September 2013x-apple-data-detectors://2

Description

A Wellcome Trust-funded postdoctoral position is available immediately in the 
laboratory of Professor Laurence Pearl FRS 
http://www.sussex.ac.uk/lifesci/pearllab/  in the MRC Genome Damage and 
Stability Centre at Sussex University, to study the structural basis for client 
protein recruitment and activation by the Hsp90 molecular chaperone system.  We 
are very well equipped for all aspects of modern structural biology, with 
state-of-the-art laboratories for molecular biology, recombinant expression in 
bacterial and eukaryotic systems, biochemistry, biophysics and X-ray 
crystallography. Excellent synchrotron access (~2 days/month) is available 
through rolling beam allocation programmes at Diamond and ESRF.

Applicants must have a PhD, and experience in recombinant expression and 
protein purification. Experience of crystallisation and X-ray crystallography 
would be an advantage. Information on our previous work in this field can be 
found at by searching PubMed (pearl [au] and hsp90).

The postholder will be responsible for expression, purification, 
crystallization and structural analysis of protein complexes involving Hsp90, 
co-chaperones and client proteins that depend on Hsp90 for their biological 
function.

Informal enquiries can be made to Professor Pearl - 
laurence.pe...@sussex.ac.ukmailto:laurence.pe...@sussex.ac.uk.

Ref 297 Further particulars including person specification [PDF 
78.54KB]http://www.sussex.ac.uk/aboutus/documents/297-fps.pdf

Essential information
Terms and conditions

Research Faculty Terms and Conditions SUMMARY [DOC 
36.00KB]http://www.sussex.ac.uk/humanresources/documents/research-summ

Application

Application form - academic post [DOC 
301.50KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.doc
Application form - academic post [PDF 
57.04KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.pdf

Please send completed application form by email 
to:lifescirecruitm...@sussex.ac.ukmailto:lifescirecruitm...@sussex.ac.uk

When emailing your application please use the following format in the 'subject' 
line: (post reference number / post title / your name). If you are applying for 
more than one post advertised by the University, please send a separate email 
and application form for each post. Please attach your completed application 
form and any other documents directly to the email rather than using a 
web-based upload / weblink service (e.g. SkyDrive) otherwise we may not receive 
your application due to incompatibility with our email software.

Or post to: Human Resources Division, Sussex House, University of Sussex, 
Falmer, Brighton, BN1 9RH.

Applications should be received by midnight on the closing date.


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544: PA +44-(0)1273 872 699

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

[ccp4bb] Postdoc in Pearl Lab on Hsp90 complexes

[Laurence Pearl]

Postdoctoral Research Fellow in Structural Biology of Hsp90 Complexes (Fixed 
term) Ref 297

School of Life Sciences - Genome Damage and Stability Centre

Full time, fixed term for 36 months
Salary range: starting at £30,424 and rising to £36,298 per annum.
Closing date for applications: 27 September 2013

Description

A Wellcome Trust-funded postdoctoral position is available immediately in the 
laboratory of Professor Laurence Pearl FRS 
http://www.sussex.ac.uk/lifesci/pearllab/  in the MRC Genome Damage and 
Stability Centre at Sussex University, to study the structural basis for client 
protein recruitment and activation by the Hsp90 molecular chaperone system.  We 
are very well equipped for all aspects of modern structural biology, with 
state-of-the-art laboratories for molecular biology, recombinant expression in 
bacterial and eukaryotic systems, biochemistry, biophysics and X-ray 
crystallography. Excellent synchrotron access (~2 days/month) is available 
through rolling beam allocation programmes at Diamond and ESRF.

Applicants must have a PhD, and experience in recombinant expression and 
protein purification. Experience of crystallisation and X-ray crystallography 
would be an advantage. Information on our previous work in this field can be 
found at by searching PubMed (pearl [au] and hsp90).

The postholder will be responsible for expression, purification, 
crystallization and structural analysis of protein complexes involving Hsp90, 
co-chaperones and client proteins that depend on Hsp90 for their biological 
function.

Informal enquiries can be made to Professor 
pearllaurence.pe...@sussex.ac.ukmailto:laurence.pe...@sussex.ac.uk.

Ref 297 Further particulars including person specification [PDF 
78.54KB]http://www.sussex.ac.uk/aboutus/documents/297-fps.pdf

Essential information
Terms and conditions

Research Faculty Terms and Conditions SUMMARY [DOC 
36.00KB]http://www.sussex.ac.uk/humanresources/documents/research-summ

Application

Application form - academic post [DOC 
301.50KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.doc
Application form - academic post [PDF 
57.04KB]http://www.sussex.ac.uk/humanresources/documents/acadnw.pdf

Please send completed application form by email 
to:lifescirecruitm...@sussex.ac.ukmailto:lifescirecruitm...@sussex.ac.uk

When emailing your application please use the following format in the 'subject' 
line: (post reference number / post title / your name). If you are applying for 
more than one post advertised by the University, please send a separate email 
and application form for each post. Please attach your completed application 
form and any other documents directly to the email rather than using a 
web-based upload / weblink service (e.g. SkyDrive) otherwise we may not receive 
your application due to incompatibility with our email software.

Or post to: Human Resources Division, Sussex House, University of Sussex, 
Falmer, Brighton, BN1 9RH.

Applications should be received by midnight on the closing date.


Laurence H. Pearl PhD FRS FMedSci

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544tel:+44-(0)1273%20876%20544: PA+44-(0)1273 872 
699tel:+44-(0)1273%20872%20699

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

[ccp4bb] DNA Repair Postdoc in Pearl lab at Sussex

[Laurence Pearl]

Postdoctoral Research Fellow

Structural Biology of DNA Repair Complexes - Ref: 120

 

CR-UK DNA Repair Enzymes Group

Genome Damage and Stability Centre

School of Life Sciences

 

UNIVERSITY OF SUSSEX

A Cancer Research UK-funded Postdoctoral position is available immediately in 
the laboratory of Professor Laurence Pearl FRS to study the structural basis 
for assembly and specificity of multi-protein complexes involved in the 
recognition and repair of DNA breaks. The internationally renowned Genome 
Damage and Stability Centre and the School of Life Sciences is very well 
equipped for all aspects of modern structural biology, with state-of-the-art 
laboratories for molecular biology, recombinant expression in bacterial and 
eukaryotic systems, biochemistry, biophysics and X-ray crystallography. 
Excellent synchrotron access (~ 2 days/month) is available through rolling 
beam-allocation programmes at Diamond and ESRF.

Applicants must have a PhD, and experience in recombinant expression and 
protein purification. Experience of crystallization and X-ray crystallography 
would be an advantage. Information on our previous work in this field can be 
found by searching PubMed (pearl lh [au]).

The postholder will be responsible for expression, purification, 
crystallization and structure determination of protein-protein and protein-DNA 
complexes, and downstream biochemical characterisation.

Fixed term to end Sept 2012 in the first instance - Full Time

Salary:  £29,853 - £35,646 per annum

Informal enquiries can be made to Professor Pearl laurence.pe...@sussex.ac.uk.

Closing date:  7 January 2011

For full details and how to apply see www.sussex.ac.uk/jobs

The University of Sussex is committed to equality of opportunity


Laurence H. Pearl PhD FMedSci FRS

Professor of Structural Biology and Head of School of Life Sciences
University of Sussex, Brighton, BN1 9RH, UK

Phone +44-(0)1273 876 544: PA +44-(0)1273 872 699 

Live Modestly and do Serious Things .. 
- Dorothy Crowfoot Hodgkin

[ccp4bb] Postdoc in Structure-Based Drug Development

[Laurence Pearl]

The Institute of Cancer Research
Section of Structural Biology
Chester Beatty Laboratories
Chelsea, London

Postdoctoral Training Fellow 
Structure-Based Development of Tankyrase Inhibitors


The Institute of Cancer Research (a College of the University of London)
is a world-class cancer research organisation.  In the 2008 Higher
Education Funding Councils' Research Assessment Exercise, The ICR
received the highest percentage of research assessed as four stars,
world leading, in the areas of biological sciences and cancer studies. 
We came top of the Times Higher Education overall table of HEIs  that
submitted to more than one UoA - ranking it as the UK's leading academic
research centre, with 37% of activity ranked 4* (world leading) and 42%
ranked 3* (internationally excellent).  

The Section of Structural Biology at ICR is exceptionally well equipped
for all aspects of modern structural biology, with state-of-the-art
laboratories for molecular biology, recombinant expression in bacterial
and eukaryotic systems, biochemistry, X-ray crystallography and electron
microscopy. Excellent synchrotron access (~ 2 days/month) is available
through rolling beam allocation programmes at Diamond and ESRF.
Applicants must have a PhD, and experience in recombinant expression,
protein purification, crystallisation and X-ray crystallography.
Information on our previous work in this field can be found by searching
PubMed and via the ICR Website http://www.icr.ac.uk/index.shtml

A Postdoctoral position funded by the Wellcome Trust Strategic Drug
Development Initiative is available immediately in the laboratory of
Professor Laurence Pearl FRS, to analyse complexes of novel
small-molecules with the transcriptional regulatory enzyme, tankyrase.

The postholder will be responsible for expression and purification of
the target protein constructs, and for co-crystallization and structural
analysis of complexes of tankyrase with novel inhibitors, emerging from
the work of the collaborating laboratories. 

Applicants should hold a PhD in a biological or chemical science, have
experience of macromolecular crystallography and recombinant protein
expression with evidence of a good publication record.  Experience in
structure-based drug development and of working in a multidisciplinary
project team, would be an advantage.

The starting salary for the position will be in the range £26,996 to
£33,188 p.a. inclusive (based on previous post-doctoral experience).
Informal enquiries can be made to Professor Laurence Pearl
(laurence.pe...@icr.ac.uk). Please note – this address is for enquiries
ONLY and CVs must be submitted in line with the instructions below.

For further particulars and details of how to apply, please visit our
website at www.icr.ac.uk   Alternatively you may call our 24 hour
recruitment line on 020 7153 5475 quoting reference number C341.

Closing date:   Monday 10th May 2010  



Laurence H. Pearl PhD FMedSci FRS

Professor of Protein Crystallography and Section Chairman
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
Phone +44-(0)20 7153 5422 : Secretary +44-(0)20 7153 5443 : FAX  
+44-(0)20 7153 5457

Live Simply and do Serious Things .. 
- Dorothy Crowfoot Hodgkin


The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

[ccp4bb] Postdoc on Hsp90 Complexes

[Laurence Pearl]

Postdoctoral Training Fellow in Structural Biology of Hsp90 Complexes

The Institute of Cancer Research, Section of Structural Biology,  
Chester Beatty Laboratories, Chelsea, London


The Institute of Cancer Research (a College of the University of  
London) is a world-class cancer research organisation.  In the 2008  
Higher Education Funding Councils' Research Assessment Exercise, The  
ICR received the highest percentage of research assessed as four  
stars, world leading, in the areas of biological sciences and cancer  
studies.  We came top of the Times Higher Education overall table of  
HEIs  that submitted to more than one UoA - ranking it as the UK's  
leading academic research centre, with 37% of activity ranked 4*  
(world leading) and 42% ranked 3* (internationally excellent).


The Section of Structural Biology at ICR is exceptionally well  
equipped for all aspects of modern structural biology, with state-of- 
the-art laboratories for molecular biology, recombinant expression in  
bacterial and eukaryotic systems, biochemistry, X-ray crystallography  
and electron microscopy. Excellent synchrotron access (~ 2 days/month)  
is available through rolling beam allocation programmes at Diamond and  
ESRF. Applicants must have a PhD, and experience in recombinant  
expression, protein purification, crystallisation and X-ray  
crystallography. Information on our previous work in this field can be  
found by searching PubMed and via the ICR Website http://www.icr.ac.uk/index.shtml


A Wellcome Trust-funded Postdoctoral position is available immediately  
in the laboratory of Professor Laurence Pearl FRS to study the  
structural basis for collaboration between the Hsp90 and Hsp70  
molecular chaperone systems in regulation and activation of ‘client’  
proteins.


The postholder will be responsible for expression, purification,  
crystallization and structural analysis of protein complexes involving  
the Hsp90 and Hsp70 molecular chaperones, co-chaperones and client  
proteins that depend on Hsp90 for their biological function.


Applicants should hold a PhD in a biological science, have experience  
of macromolecular crystallography and recombinant protein expression,  
and have evidence of a good publication record.  Experience in single  
particle electron microscopy would be an advantage.


The starting salary for the position will be in the range £26,996 to  
£35,518 p.a. inclusive (based on previous post-doctoral experience).  
Informal enquiries can be made to Professor Laurence Pearl (laurence.pe...@icr.ac.uk 
). Please note – this address is for enquiries ONLY and CVs must be  
submitted in line with the instructions below.


For further particulars and details of how to apply, please visit our  
website at www.icr.ac.uk   Alternatively you may call our 24 hour  
recruitment line on 020 7153 5475 quoting reference number C334.


Closing date:Friday 5th March 2010

--
  Laurence H. Pearl PhD FMedSci FRS

  Professor of Protein Crystallography and Section Co-Chairman
  Section of Structural Biology, Institute of Cancer Research
  Chester Beatty Laboratories,  237 Fulham Road, London SW3 6JB, UK

  Phone +44-(0)20 7153 5422 : Secretary +44-(0)20 7153 5443
  FAX   +44-(0)20 7153 5457 : E-Mail laurence.pe...@icr.ac.uk
--
   Live Simply and do Serious Things ..  - Dorothy Crowfoot Hodgkin
--




The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

[ccp4bb] Postdoc in Structural Biology of DNA Repair

[Laurence Pearl]

Postdoctoral Training Fellow

Structural Biology of DNA Damage Signalling and Repair

Cancer Research UK DNA Repair Enzyme Group

Section of Structural Biology, The Institute of Cancer Research,  
Chester Beatty Laboratories, Chelsea, London




The Institute of Cancer Research is one of the world's leading cancer  
research organisations and is internationally renowned for the quality  
of its science.  Our mission is the relief of human suffering by  
pursuing excellence in the fight against cancer.


A Cancer Research UK-funded Postdoctoral position is available in the  
laboratory of Professor Laurence Pearl FRS in the Section of  
Structural Biology, to study the structural basis for specificity and  
assembly of proteins and complexes involved in recognition, signalling  
and repair of DNA damage. The Section is exceptionally well equipped  
for all aspects of modern structural biology, with state-of-the-art  
laboratories for molecular biology, biochemistry, X-ray  
crystallography and electron microscopy. Applicants must have a PhD,  
and experience in recombinant expression and protein purification, and  
crystallisation and X-ray crystallography and/or single particle  
electron microscopy. Information on our previous work in this field  
can be found by searching PubMed and via our Home Page http://www.icr.ac.uk/structbi/


The starting salary for the positions will be in the range £26,966 to  
£37,476 p.a. inclusive (based on previous post-doctoral experience)  
and the posts are offered initially on a fixed term contract from 1st  
January 2010 until end of September 2012. Informal enquiries to laurence.pe...@icr.ac.uk


To apply, please send a copy of your CV, including the names and  
addresses of two referees, by E-mail to recruitm...@icr.ac.uk or by  
post to the HR Office, The Institute of Cancer Research, 123 Old  
Brompton Road, London SW7 3RP, quoting reference C304.  For a job  
description and person specification please visit our website at: www.icr.ac.uk/jobs.html 
Alternatively, you may call our 24 hr recruitment line on 020 7153  
5475.


Closing date: 23rd November 2009

--
  Laurence H. Pearl PhD FMedSci FRS

  Professor of Protein Crystallography and Section Co-Chairman
  Section of Structural Biology, Institute of Cancer Research
  Chester Beatty Laboratories,  237 Fulham Road, London SW3 6JB, UK

  Phone +44-(0)20 7153 5422 : Secretary +44-(0)20 7153 5443
  FAX   +44-(0)20 7153 5457 : E-Mail laurence.pe...@icr.ac.uk
--
   Live Simply and do Serious Things ..  - Dorothy Crowfoot Hodgkin
--




The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

[ccp4bb] Postdoc - Structural Biology of Hsp90 Complexes

[Laurence Pearl]

   Postdoctoral Training Fellow - Structural Biology of Hsp90 Complexes

Section of Structural Biology, ICR
Chester Beatty Laboratories
Chelsea, London, UK

A Wellcome Trust-funded Postdoctoral position is available from 1st 
October 2009 in the laboratory of Professor Laurence Pearl FRS, in the 
Section of Structural Biology, to study the structural basis for 
regulation and activation of 'client' proteins by the Hsp90 molecular 
chaperone system. The Postdoc will be responsible for expression, 
purification, crystallization and structural analysis of protein 
complexes involving the Hsp90 molecular chaperone, its co-chaperones and 
client proteins that depend on Hsp90 for their biological function.


The Institute of Cancer Research (a College of the University of London) 
is a world-class cancer research organization receiving the highest 
rating in the 2008 RAE. In partnership with The Royal Marsden NHS 
Foundation Trust, we form the largest comprehensive cancer centre in 
Europe, dedicated to research that extends from world leading basic 
science in epidemiology, genetics and structural and molecular biology, 
through drug discovery and development, to cancer diagnosis and patient 
treatment.


The Section of Structural Biology at ICR is exceptionally well equipped 
for all aspects of modern structural biology, with state-of-the-art 
laboratories for molecular biology, recombinant expression in bacterial 
and eukaryotic systems, biochemistry, X-ray crystallography and electron 
microscopy. Excellent synchrotron access (~ 2 days/month) is available 
through rolling beam allocation programmes at Diamond and ESRF. 
Applicants must have a PhD, and experience in recombinant expression, 
protein purification, crystallisation and X-ray crystallography. 
Information on our previous work in this field can be found by searching 
PubMed and via the ICR Website http://www.icr.ac.uk/index.shtml


The starting salary for the position will be in the range £26,966 to 
£35,518 p.a. inclusive (based on previous post-doctoral experience) and 
the post is offered initially on a fixed term contract of up to 2 years. 
Informal enquiries can be made to Professor Laurence Pearl 
(laurence.pe...@icr.ac.uk). Please note -- this address is for enquiries 
ONLY and CVs must be submitted in line with the instructions below.



To apply, please send two copies of your CV, including the names and 
addresses of two referees to the HR Office, The Institute of Cancer 
Research, 123 Old Brompton Road, London SW7 3RP, quoting reference C 
219  For a job description and person specification please visit our 
website at: www.icr.ac.uk/jobs.html. Alternatively, you may call our 24 
hr recruitment line on 020 7153 5475.


Closing date: 8 May 2009

--
-
Laurence H. Pearl PhD FMedSci FRS 


Professor of Protein Crystallography and Section Chairman
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB
Phone +44-(0)20 7153 5422 : Secretary 5443 : FAX 5457
-
Live Simply and do Serious Things .. 
- Dorothy Crowfoot Hodgkin
-



The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

[ccp4bb] Postdocs in DNA damage signalling/repair at ICR

[Laurence Pearl]

Postdoctoral Training Fellows 

Structural Biology of DNA Damage Signalling and Repair 

Cancer Research UK DNA Repair Enzyme Group
Section of Structural Biology
The Institute of Cancer Research
Chester Beatty Laboratories
Chelsea, London


The Institute of Cancer Research is one of the world's leading cancer research 
organisations and is internationally renowned for the quality of its science.  
Our mission is the relief of human suffering by pursuing excellence in the 
fight against cancer.

Two Cancer Research UK-funded Postdoctoral positions are available in Professor 
Laurence Pearl’s group in the Section of Structural Biology, to study the 
structural basis for specificity and assembly of proteins and complexes 
involved in recognition, signalling and repair of DNA damage. The Section is 
exceptionally well equipped for all aspects of modern structural biology, with 
state-of-the-art laboratories for molecular biology, biochemistry, X-ray 
crystallography and electron microscopy. 

Applicants must have a PhD in a biological science, and experience in 
recombinant expression and protein purification, and crystallisation and X-ray 
crystallography and/or single particle electron microscopy. 

Information on our previous work can be found by searching PubMed and via our 
Home Page: 
http://www.icr.ac.uk/research/research_sections/structural_biology/index.shtml  

The starting salary for the positions will be in the range £29,256 to £33,845 
p.a. inclusive (based on previous post-doctoral experience) and the posts are 
offered initially on a fixed term contract of up to 3 years. 

Informal enquiries are welcome and can be made to Prof. Laurence Pearl ([EMAIL 
PROTECTED]). Please note * this address is for enquiries only and CVs must be 
submitted in line with the instructions below.

For further particulars and details of how to apply, please visit our website 
at www.icr.ac.uk   Alternatively, you may call our 24 hour recruitment line on 
020 7153 5475 quoting reference number C161.

Closing date: Friday 22 August 2008



Laurence H. Pearl PhD FRS FMedSci

Professor of Protein Crystallography and Section Chairman
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
Phone +44-(0)20 7153 5422 : Secretary +44-(0)20 7153 5443 : FAX   +44-(0)20 
7153 5457

Live Simply and do Serious Things .. 
- Dorothy Crowfoot Hodgkin


The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

Re: [ccp4bb] Broken Masks

[Laurence Pearl]

I think it is a version bug of some sort - when I ran the same mask 
creation run in CCP4i on my MacBook laptop (which has the most recent 
CCP4) it displays fine and also worked perfectly in dm averaging, 
turning a tangled mess into interpretable density.


The mask generated by NCSMASK on my desktop Mac displayed weirdly in 
COOT on that machine, and crashed COOT on the laptop as soon as I treid 
to display it.


Anyway the job is done - thanks for the advice.

Laurence

Daniel Anderson wrote:

Could it be that NCSMASK folded the mask back into an asymmetric unit?
My NCSMASK input file contains explicit xyzlim boundaries and notrim 
keyword.


On Tue, 18 Mar 2008, Laurence Pearl wrote:

I'm trying to generate a mask around a molecule using NCSMASK under 
the CCP4i interface, for ultimate use in NCS averaging and density 
modification in dm. Being a little old-fashioned I wanted to check 
that it covered the required molecule. However when displayed in 
COOT, there seem to be arbitary flat slabs running through the mask, 
with some sort of inversion or other transformation on the other side 
of the slab. The mask has a similar appearance in the CCP4mg. The 
flattenings are parallel to cell edges, and sometimes, but not always 
adjacent to cell faces.


Is this a known problem ? is it NCSMASK ? or is it COOT and CCP4mg ?






--
-
Laurence H. Pearl PhD FMedSci

Professor of Protein Crystallography and Section Chairman
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB
Phone +44-(0)20 7153 5422 : Secretary 5443 : FAX 5457
-
Live Simply and do Serious Things .. 
- Dorothy Crowfoot Hodgkin
-


The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

[ccp4bb] Broken Masks

[Laurence Pearl]

I'm trying to generate a mask around a molecule using NCSMASK under the 
CCP4i interface, for ultimate use in NCS averaging and density 
modification in dm. Being a little old-fashioned I wanted to check that 
it covered the required molecule. However when displayed in COOT, there 
seem to be arbitary flat slabs running through the mask, with some sort 
of inversion or other transformation on the other side of the slab. The 
mask has a similar appearance in the CCP4mg. The flattenings are 
parallel to cell edges, and sometimes, but not always adjacent to cell 
faces.


Is this a known problem ? is it NCSMASK ? or is it COOT and CCP4mg ?

--
-
Laurence H. Pearl PhD FMedSci

Professor of Protein Crystallography and Section Chairman
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB
Phone +44-(0)20 7153 5422 : Secretary 5443 : FAX 5457
-
Live Simply and do Serious Things .. 
- Dorothy Crowfoot Hodgkin
-


The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

This e-mail message is confidential and for use by the addressee only.  If the 
message is received by anyone other than the addressee, please return the 
message to the sender by replying to it and then delete the message from your 
computer and network.

[ccp4bb] Post-Doc at Institute of Cancer Research, Chester Beatty Labs, London

[Laurence Pearl]

The Institute of Cancer Research (University of London)

Cancer Research UK Centre for Cell and Molecular Biology
 Section of Structural Biology

Chester Beatty Laboratories - Chelsea, London

Post-doctoral Training Fellow

Structure and Interactions of the Retinoblastoma-associated Protein

Applications are invited for a Post-doctoral position funded by Cancer Research 
UK, to work on structure and interactions of domains of the 
retinoblastoma-associated protein pRb, implicated in regulation of DNA 
replication and apoptosis (see Hassler et al, 2007, Mol. Cell, 28, 371-385).  

The project is a collaboration between the teams led by  Dr. Sibylle Mittnacht 
(Cancer Research UK Programme on tumour cell signalling networks) and Prof. 
Laurence Pearl (Structural Biology) at the Chester Beatty Laboratories. Between 
them these teams share an extensive range of state of the art equipment for 
biochemical, biophysical and structural work. The laboratories are 
exceptionally well equipped for all aspects of modern cell biology and 
structural biology, with state-of-the-art equipment for cell imaging, mass 
spectrometry, X-ray crystallography and structural electron micoscopy.

Applicants should hold a PhD in Macromolecular Crystallography or Protein 
biochemistry with postdoctoral experience in recombinant expression, protein 
purification and, ideally, crystallographic structure determination.

Protein biochemistry, protein crystallisation and computational crystallography 
skills are essential and candidates with a working knowledge of eukaryotic cell 
biology, cell-cycle control, DNA replication survival and apoptosis will be at 
an advantage.

The Institute of Cancer Research (a College of the University of London) is a 
world-class cancer research organization with HEFCE RAE ratings of 
international excellence across all of its research programmes.  In partnership 
with The Royal Marsden NHS Foundation Trust, we form the largest comprehensive 
cancer centre in Europe, dedicated to research that extends from epidemiology, 
genetics and molecular biology, through drug discovery and development, to 
cancer diagnosis and patient treatment.  This makes us uniquely placed to work 
towards our vision that people may live their lives free from the fear of 
cancer as a life threatening disease.

The post is offered on a fixed-term contract with a starting salary in the 
range £29,256 to £36,922 p.a. inclusive based on relevant skills and 
experience. 

Informal enquiries to Dr Sibylle Mittnacht ([EMAIL PROTECTED]) or Prof. 
Laurence Pearl ([EMAIL PROTECTED]) are encouraged but DO NOT send your 
application to this email address. Please submit applications along with names 
of two references quoting reference number C125.

For further particulars and details of how to apply, please visit our website 
at www.icr.ac.uk   Alternatively you may call our 24 hour recruitment line on 
020 7153 5475 quoting reference number C125.

Closing date:   31st March 2008  




Laurence H. Pearl PhD FMedSci

Professor of Protein Crystallography and Section Chairman
Section of Structural Biology, Institute of Cancer Research
Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK
Phone +44-(0)20 7153 5422 : Secretary +44-(0)20 7153 5443 : FAX   +44-(0)20 
7153 5457

Live Simply and do Serious Things .. 
- Dorothy Crowfoot Hodgkin


The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company 
Limited by Guarantee, Registered in England under Company No. 534147 with its 
Registered Office at 123 Old Brompton Road, London SW7 3RP.

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