Re: [ccp4bb] Can Mathew's coefficient tell about a complex
If you want to know what in your crystal, run SDS-PAGE of your crystal. On Thu, Dec 5, 2013 at 6:39 AM, Kris Tesh kris.t...@att.net wrote: If either of the two protein structures has been determined/deposited, I would check if your unit cell matched one of them. Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston -- *From:* Tanner, John J. tanne...@missouri.edu *To:* CCP4BB@JISCMAIL.AC.UK *Sent:* Wednesday, December 4, 2013 4:29 PM *Subject:* Re: [ccp4bb] Can Mathew's coefficient tell about a complex First of all, there are two Ts and no apostrophe in Matthews. The method is based on the following paper, which is worth reading. It is one of the first examples, possibly the first, of structural bioinformatics. J Mol Biol. 1968 Apr 28;33(2):491-7. Solvent content of protein crystals. Matthews BW. http://www.ncbi.nlm.nih.gov/pubmed/5700707 Secondly, you might run a gel on your crystals. John J. Tanner Professor of Biochemistry and Chemistry University of Missouri-Columbia 125 Chemistry Building Columbia, MO 65211 Phone: 573-884-1280 Fax: 573-882-2754 Email: tanne...@missouri.edu http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html On Dec 4, 2013, at 4:06 PM, abbas maqbool abbas_maqb...@yahoo.com wrote: Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa. Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 Da), and results were like that Cell Volume = 993567 Nmol/asymMathews coeff%solvent 13.94 68 21.9737 3 1.36 Can any one please explain what does it suggest? Thanks Abbas
[ccp4bb] Can Mathew's coefficient tell about a complex
Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa. Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 Da), and results were like that Cell Volume = 993567 Nmol/asym Mathews coeff %solvent 1 3.94 68 2 1.97 37 3 1.3 6 Can any one please explain what does it suggest? Thanks Abbas
Re: [ccp4bb] Can Mathew's coefficient tell about a complex
Looks like 1 or 2 protein units per ASU. Two seems most likely, but I have had a bunch of crystals with 67% solvent, too, so 1 is not out of the question if less probable. So try both with your favorite MR method and see what happens. Crystal packing in the MR solution will tell all. ___ Roger S. Rowlett Gordon Dorothy Kline Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu On 12/4/2013 5:06 PM, abbas maqbool wrote: Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa. Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 Da), and results were like that Cell Volume = 993567 Nmol/asymMathews coeff%solvent 13.94 68 21.9737 31.36 Can any one please explain what does it suggest? Thanks Abbas
Re: [ccp4bb] Can Mathew's coefficient tell about a complex
First of all, there are two Ts and no apostrophe in Matthews. The method is based on the following paper, which is worth reading. It is one of the first examples, possibly the first, of structural bioinformatics. J Mol Biol. 1968 Apr 28;33(2):491-7. Solvent content of protein crystals. Matthews BW. http://www.ncbi.nlm.nih.gov/pubmed/5700707 Secondly, you might run a gel on your crystals. John J. Tanner Professor of Biochemistry and Chemistry University of Missouri-Columbia 125 Chemistry Building Columbia, MO 65211 Phone: 573-884-1280 Fax: 573-882-2754 Email: tanne...@missouri.edumailto:tanne...@missouri.edu http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html On Dec 4, 2013, at 4:06 PM, abbas maqbool abbas_maqb...@yahoo.commailto:abbas_maqb...@yahoo.com wrote: Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa. Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 Da), and results were like that Cell Volume = 993567 Nmol/asymMathews coeff%solvent 13.94 68 21.9737 3 1.36 Can any one please explain what does it suggest? Thanks Abbas
Re: [ccp4bb] Can Mathew's coefficient tell about a complex
If either of the two protein structures has been determined/deposited, I would check if your unit cell matched one of them. Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston From: Tanner, John J. tanne...@missouri.edu To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, December 4, 2013 4:29 PM Subject: Re: [ccp4bb] Can Mathew's coefficient tell about a complex First of all, there are two Ts and no apostrophe in Matthews. The method is based on the following paper, which is worth reading. It is one of the first examples, possibly the first, of structural bioinformatics. J Mol Biol. 1968 Apr 28;33(2):491-7. Solvent content of protein crystals. Matthews BW. http://www.ncbi.nlm.nih.gov/pubmed/5700707 Secondly, you might run a gel on your crystals. John J. Tanner Professor of Biochemistry and Chemistry University of Missouri-Columbia 125 Chemistry Building Columbia, MO 65211 Phone: 573-884-1280 Fax: 573-882-2754 Email: tanne...@missouri.edu http://faculty.missouri.edu/~tannerjj/tannergroup/tanner.html On Dec 4, 2013, at 4:06 PM, abbas maqbool abbas_maqb...@yahoo.com wrote: Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa. Actually I calculated Mathew's coeffient (based on Mol. wt of complex 42000 Da), and results were like that Cell Volume = 993567 Nmol/asym Mathews coeff %solvent 1 3.94 68 2 1.97 37 3 1.3 6 Can any one please explain what does it suggest? Thanks Abbas
