[ccp4bb] DLS/CCP4 data analysis workshop 2023
We are pleased to announce that the 10th joint Diamond-CCP4 Workshop on MX data collection and structure solution is now open for applications! Bringing together leading experts in the field of MX to teach best practice in data collection and analysis, this course is aimed at PhD students, postdocs and early career scientists who have a focus on structural biology. This is again an in-person workshop, based at Diamond Light Source in the UK, but having learned from the online-only workshops during the pandemic, we will keep two remote setup days prior to the on-site element, and we will make use of a Slack workspace through the course. It is essential that applicants commit to attending the workshop in its entirety. Please note below the workshop days and timings involved: - First online preparation day (before fishing crystals): Monday 6 November - Second online preparation day (before data collection): Tuesday 21 November - On-site: Monday 27 November to Tuesday 5 December - Day off: Saturday 2 December There is no fee to attend this online workshop, however attendance will be subject to an application process and a letter of support from the attendee's supervisor will be required. Both the application form and supervisor's letter of support will need to be submitted by 17:00 (UK time) on 9 October 2023. Some prior experience of crystallography and data collection is expected, and those who already have an interesting project (crystals and possibly previously collected datasets) will be given priority in selection. - The course homepage: https://www.ccp4.ac.uk/schools/DLS-2023/ - Apply at the Diamond website: https://www.diamond.ac.uk/Home/Events/2023/Diamond-CCP4-Data-Collection-and-Structure-Solution-Workshop-2023.html -- David (on behalf of the organisers) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] DLS/CCP4 data analysis workshop 2022
We are pleased to announce that the 9th joint Diamond-CCP4 Workshop on MX data collection and structure solution is now open for applications. Bringing together leading experts in the field of MX to teach best practice in data collection and analysis, this course is aimed at PhD students, postdocs and early career scientists who have a focus on structural biology. After the last two remote events limited to online-only using Zoom, we are very happy to report that we intend to go back to an in-person workshop this year! However, having learned from the online-only workshops, we will keep two remote setup days prior to the on-site element, and we will make use of a Slack workspace through the course. It is essential that applicants commit to attending the workshop in its entirety. Please note below the workshop days and timings involved: - First online preparation day (before fishing crystals): Monday 17 October - Second online preparation day (before data collection): Tuesday 22 November - On-site: Monday 28 November to Tuesday 6 December - Day off: Saturday 3 December There is no fee to attend this online workshop, however attendance will be subject to an application process and a letter of support from the attendee's supervisor will be required. Both the application form and supervisor's letter of support will need to be submitted by 17:00 (UK time) on 20 September 2022. Some prior experience of crystallography and data collection is expected, and those who already have an interesting project (crystals and possibly previously collected datasets) will be given priority in selection. - The course homepage: https://www.ccp4.ac.uk/schools/DLS-2022/ - Apply at the Diamond website: https://www.diamond.ac.uk/Home/Events/2022/Diamond-CCP4-Data-Collection-and-Structure-Solution-Workshop-2022.html -- David (on behalf of the organisers) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] DLS/CCP4 data analysis workshop 2021
We are pleased to announce that the 8th joint Diamond-CCP4 Workshop on MX data collection and structure solution is now open for applications. Bringing together leading experts in the field of MX to teach best practice in data collection and analysis, this course is aimed at PhD students, postdocs and early career scientists who have a focus on structural biology. The workshop will take place online via Zoom over the course of two full weeks, plus a preparation day in advance of the workshop. It is essential that applicants commit to attending the workshop in its entirety. Please note below the workshop days and timings involved: - Preparation day: Tuesday 23 November 2021 - Week 1: Monday 29 November to Friday 3 December 2021 - Week 2: Monday 6 December to Friday 10 December 2021 - The days will generally begin at 08:45 and will finish at 17:15 (UK time) There is no fee to attend this online workshop, however attendance will be subject to an application process and a letter of support from the attendee's supervisor will be required. Both the application form and supervisor's letter of support will need to be submitted by 17:00 (UK time) on Friday 1 October 2021 (though see disclaimer below). Some prior experience of crystallography and data collection is expected, and those who already have an interesting project (crystals and possibly previously collected datasets) will be given priority in selection. - The course homepage: https://www.ccp4.ac.uk/schools/DLS-2021/ - Apply at the Diamond website: https://www.diamond.ac.uk/Home/Events/2021/Diamond-CCP4-Data-Collection-and-Structure-Solution-Workshop-2021.html *Disclaimer* Depending on the number of submissions we receive, we may need to close applications earlier than the stated deadline to allow the organisers time to process them. Applicants are therefore encouraged to apply as soon as possible with all the required details requested, including the letter of support. Incomplete applications will not be processed. -- David (on behalf of the organisers) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] DLS/CCP4 Data Collection and Structure Solution Workshop, 30 Nov - 11 Dec 2020
We are pleased to announce that the 7th joint Diamond-CCP4 Workshop on MX data collection and structure solution is open for applications. Bringing together leading experts in the field of MX to teach best practice in data collection and analysis, this course is aimed at PhD students, postdocs and early career scientists who have a focus on structural biology. The workshop will take place online via Zoom over the course of two full weeks, as well as a preparation day in advance of the workshop. It is essential that applicants commit to attending the workshop in its entirety. Please note below the workshop days and timings involved: - Preparation day: Monday 23 November 2020 - Week 1: Monday 30 November to Friday 4 December 2020 - Week 2: Monday 7 December to Friday 11 December 2020 Each day will begin at 08:45 and will finish at 17:15 (GMT - UK Time) Some prior experience of crystallography and data collection is expected, and those who can bring an interesting project with them (crystals and possibly previously collected datasets) will be given priority. There is no fee to attend this online workshop, however attendance will be subject to an application process and a letter of support from the attendee's supervisor will be required. Both the application form and supervisor's letter of support will need to be submitted by 17:00 (GMT) on Friday 16 October 2020. - The course homepage: https://www.ccp4.ac.uk/schools/DLS-2020/ - Apply at the Diamond website: https://www.diamond.ac.uk/Home/Events/2020/Diamond-CCP4-Data-Collection-and-Structure-Solution-Workshop-2020.html *Disclaimer* Depending on the number of submissions we receive, we may need to close applications earlier than the deadline to allow the organisers time to process them. Applicants are therefore encouraged to apply as soon as possible with all the required details requested, including the letter of support - any incomplete applications will not be processed. -- David (on behalf of the organisers) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] DLS/CCP4 data analysis workshop 2019
PhD students, postdocs and early career scientists, Please consider applying for the sixth joint DLS/CCP4 workshop on MX data collection and structure solution, to be held at Diamond Light Source, UK from the 1st to the 8th December 2019. This course offers the opportunity for you to work alongside leaders in the field of MX on data from your own crystals. For more details please check here: http://www.ccp4.ac.uk/schools/DLS-2019/ The workshop has been very successful in previous years, as shown by acknowledgements given in publications from attendees of the course: http://www.ccp4.ac.uk/schools/DLS-school/index.php The application period is now open and will* close on the 18th October*. Applicants will be required to submit a CV/resume, describe their projects and obtain a letter of support from a supervisor, so please do not wait until the last minute to apply! Those with crystals and/or data will be given priority. Best wishes on behalf of the organisers, David Waterman (CCP4) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] DLS/CCP4 data analysis workshop 2018
PhD students, postdocs and early career scientists, Please consider applying for the fifth joint DLS/CCP4 workshop on MX data collection and structure solution, to be held at Diamond Light Source, UK from the 2nd to the 9th December (with accommodation from the 1st). This course offers the opportunity for you to work alongside leaders in the field of MX on data from your own crystals. For more details please check here: http://www.ccp4.ac.uk/schools/DLS-2018/ The application period is now open and will close on the *7th October*. Applicants will be required to submit a CV/resume, describe their projects and obtain a letter of support from a supervisor, so please do not wait until the last minute to apply! Those with crystals and/or data will be given priority. Best wishes on behalf of the organisers, David Waterman (CCP4) To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1
[ccp4bb] DLS/CCP4 data analysis workshop 2017
PhD students, postdocs and early career scientists, Please consider applying for the fourth joint DLS/CCP4 workshop on MX data collection and structure solution, to be held at Diamond Light Source, UK from the 30th November to the 7th December 2017. This course offers the opportunity for you to work alongside leaders in the field of MX on data from your own crystals. For more details please check here: http://www.ccp4.ac.uk/schools/DLS-2017/ The application period is now open and will close on the 6th October. Applicants will be required to submit a CV/resume, describe their projects and obtain a letter of support from a supervisor, so please do not wait until the last minute to apply! Those with crystals and/or data will be given priority. Best wishes on behalf of the organisers, David Waterman (CCP4)
[ccp4bb] DLS BAG training, 5th-6th July.
The Diamond Light Source Macromolecular Crystallography group would like to invite both our academic and industrial users to MX beamline training sessions on the 5th -6st July 2017. The aim is to provide MX users with sufficient training to be able to operate any of the Diamond MX beamlines efficiently and to get the most benefit from their beam-time. The training will involve hands-on sessions on the suite of five operational MX beamlines (http://www.diamond.ac.uk/mx-home/) as well as offline software sessions. Sessions include: 5th July afternoon session Hands on software: Two tutorial sessions: CCP4 DIALS 6st July, MX beamlines and data room sessions: MX software: automation in data analysis Multi axis goniometry / SAD Anomalous data collection Introduction to VMX-i I24 new end station/ In-situ diffraction Fragment screening – I04-1/Lab36 Experiment database: new ISPyB Registration is free-of-charge with lunch provided on the 5th and 6st July, and accommodation and dinner for the night of July 5th. Travelling expenses within the UK will also be provided. The training is targeted at all users, and is not limited to students and post docs. It is essential that each BAG sends at least one representative per calendar year. Registration Places are limited to twenty five participants. The registration deadline is on the June 25th 2017 at 11:59. Registration is now open: http://www.diamond.ac.uk/Home/Events/2017/MX-BAG-TrainingJuly2017.html For more information please contact: Dr. Pierpaolo Romano Diamond light Source Ltd. pierpaolo.rom...@diamond.ac.uk Tel. 01235 778179
Re: [ccp4bb] DLS
instead of DLS you could also look into buying a fancy PCR machine ~30K where you can perform a) regular PCR b) quantitative PCR c) thermal stability tests for your protein Ericsson et al. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Anal Biochem (2006) vol. 357 (2) pp. 289-98 Crowther et al. Buffer Optimization of Thermal Melt Assays of Plasmodium Proteins for Detection of Small-Molecule Ligands. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening (2009) pp. Sure you are missing the quantification for how homogeneous your sample is and an estimation of the molecular weight of the particles in solution but the correlation between crystallizability and DLS is not so good compared to thermal shifts as pointed out in Ericsson 2006. You will have a high end purification system so you could get a sense of your sample homogeneity via SEC instead Just a thought, Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://web.mac.com/bosch_lab/ On Oct 30, 2010, at 12:02 AM, Mohd Shukuri Mohamad Ali wrote: Hi there Sorry for the off ccp4 topics. I am planning to get a DLS to set up my crystallography lab. I got a tight budget. Around USD 8 perhaps. I'm quite new to this field. Thanks in advance for your suggestions Regards Shukuri
Re: [ccp4bb] DLS
I second the question of need: a decent PCR machine capable of 'thermofluor'-like experiments should cost around $34-$38K, which leaves about the same amount of $$ for a decent purification machine. One of the questions you have to figure out is: are you setting up a high-throughput lab or a regular kind of lab? If you're doing HT work then perhaps a plate-based DLS is a good idea, although you will undoubtedly need more $$ for the rest of the stuff. On the other hand if you're setting up to do 'regular' i.e. low-throughput (I prefer the term 'hand crafted', by the way) purification then an investment in a Thermofluor machine (be it PCR or a multiwell spectrofluorimeter with temperature control) and another useful device might be more wise. Having written this, I would have to add for the sake of honesty that my lab has both - but we are in fact straddling the boundary between HT and hand-crafted work and we have use both instruments daily. Artem On Fri, Oct 29, 2010 at 11:02 PM, Mohd Shukuri Mohamad Ali shuk...@biotech.upm.edu.my wrote: Hi there Sorry for the off ccp4 topics. I am planning to get a DLS to set up my crystallography lab. I got a tight budget. Around USD 8 perhaps. I'm quite new to this field. Thanks in advance for your suggestions Regards Shukuri
Re: [ccp4bb] DLS
Hi Shukuri, If you're on a tight budget and you only want to use DLS to verify sample homogeneity I would save my money or use it for something else (a crystallization robot, for example). You definitely do NOT need DLS to solve crystal structures, including those of membrane proteins. Many monodisperse samples do not crystallize and I can tell you from experience that polydisperse samples can crystallize perfectly well. Gel filtration chromatography is a good and much cheaper alternative to DLS, and you'll have the columns already anyway. Admittedly, DLS will give you somewhat more info than gel filtration, but again, if money is an issue I would not get a DLS instrument. For me its comparable to a UV microscope for verification of crystals; nice for sure, but not worth the $$$. Cheers, Bert On 10/30/10 12:02 AM, Mohd Shukuri Mohamad Ali shuk...@biotech.upm.edu.my wrote: Hi there Sorry for the off ccp4 topics. I am planning to get a DLS to set up my crystallography lab. I got a tight budget. Around USD 8 perhaps. I'm quite new to this field. Thanks in advance for your suggestions Regards Shukuri
Re: [ccp4bb] DLS
hear-hear wise words. may I add that if you are buying a DLS anyway, maybe consider getting a model which can also be connected online to an FPLC to do Static LS measurements, ... after all a DLS signal is basically an autocorrelation of the static signal over time ... which will tell you about dispersion, but is incapable of measuring MW, and can only get Rg, unlike static LS (MALLS) that will give you absolute MW over your FPLC - which is handy. A. On Oct 30, 2010, at 20:10, Artem Evdokimov wrote: I second the question of need: a decent PCR machine capable of 'thermofluor'-like experiments should cost around $34-$38K, which leaves about the same amount of $$ for a decent purification machine. One of the questions you have to figure out is: are you setting up a high-throughput lab or a regular kind of lab? If you're doing HT work then perhaps a plate-based DLS is a good idea, although you will undoubtedly need more $$ for the rest of the stuff. On the other hand if you're setting up to do 'regular' i.e. low-throughput (I prefer the term 'hand crafted', by the way) purification then an investment in a Thermofluor machine (be it PCR or a multiwell spectrofluorimeter with temperature control) and another useful device might be more wise. Having written this, I would have to add for the sake of honesty that my lab has both - but we are in fact straddling the boundary between HT and hand-crafted work and we have use both instruments daily. Artem On Fri, Oct 29, 2010 at 11:02 PM, Mohd Shukuri Mohamad Ali shuk...@biotech.upm.edu.my wrote: Hi there Sorry for the off ccp4 topics. I am planning to get a DLS to set up my crystallography lab. I got a tight budget. Around USD 8 perhaps. I'm quite new to this field. Thanks in advance for your suggestions Regards Shukuri P please don't print this e-mail unless you really need to Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member Department of Biochemistry (B8) Netherlands Cancer Institute, Dept. B8, 1066 CX Amsterdam, The Netherlands Tel: +31 20 512 1951 Fax: +31 20 512 1954 Mobile / SMS: +31 6 28 597791
[ccp4bb] DLS
Hi there Sorry for the off ccp4 topics. I am planning to get a DLS to set up my crystallography lab. I got a tight budget. Around USD 8 perhaps. I'm quite new to this field. Thanks in advance for your suggestions Regards Shukuri
[ccp4bb] DLS
Dear Fellow Crystallographers We are going to buy a DLS instrument to help us assess crystallizability of our proteins. I would be grateful for any opinions - either on this BB or in my mail box (in which case I'll keep them to myself, naturally). We have looked more closely at two instruments: DynaPro NanoStar from Wyatt and Zetasizer Micro V from Malvern and both seem to be rather similar in terms of parameters and price. I would appreciate user opinions as to the reliability, ease of use or any features that make a given instrument particularly suited for assessing biological samples. Many thanks, Wojtek -- -- Prof. dr hab. Wojciech Rypniewski tel: +48-61-8528503 Institute of Bioorganic Chemistry fax: +48-61-8520532 Polish Academy of Sciences e-mail: wojt...@ibch.poznan.pl Noskowskiego 12/14 www: www.man.poznan.pl/~wojtekr/ 61-704 Poznan, Poland --
Re: [ccp4bb] DLS
Hiya. My two penneth on the Malvern Zetasizer. Simple, idiot-proof operation. Software is easy and intuative. Reports are customisable to give you what information you want. Problems easy to trouble shoot IMHO. Does DLS, SLS, melting point determination. After-care support is good from Malvern. Not used the Wyatt instrument. HTH, Dave David C. Briggs PhD Father, Structural Biologist and Sceptic University of Manchester E-mail: david.c.bri...@manchester.ac.uk Internetz: http://xtaldave.posterous.com/ Twitter: @xtaldave Skype: DocDCB On 4 February 2010 13:36, Wojciech Rypniewski wojt...@ibch.poznan.pl wrote: Dear Fellow Crystallographers We are going to buy a DLS instrument to help us assess crystallizability of our proteins. I would be grateful for any opinions - either on this BB or in my mail box (in which case I'll keep them to myself, naturally). We have looked more closely at two instruments: DynaPro NanoStar from Wyatt and Zetasizer Micro V from Malvern and both seem to be rather similar in terms of parameters and price. I would appreciate user opinions as to the reliability, ease of use or any features that make a given instrument particularly suited for assessing biological samples. Many thanks, Wojtek -- -- Prof. dr hab. Wojciech Rypniewski tel: +48-61-8528503 Institute of Bioorganic Chemistry fax: +48-61-8520532 Polish Academy of Sciences e-mail: wojt...@ibch.poznan.pl Noskowskiego 12/14 www: www.man.poznan.pl/~wojtekr/ 61-704 Poznan, Poland --
Re: [ccp4bb] DLS
Hi Wojtek I can easily second Dave's comment! Best Savvas Savvas Savvides Unit for Structural Biology @ L-ProBE Ghent University K.L. Ledeganckstraat 35, 9000 Ghent, Belgium Ph. +32 (0)472 928 519 http://www.LProBE.ugent.be/xray.html On 04/02/10 14:54, David Briggs drdavidcbri...@gmail.com wrote: Hiya. My two penneth on the Malvern Zetasizer. Simple, idiot-proof operation. Software is easy and intuative. Reports are customisable to give you what information you want. Problems easy to trouble shoot IMHO. Does DLS, SLS, melting point determination. After-care support is good from Malvern. Not used the Wyatt instrument. HTH, Dave David C. Briggs PhD Father, Structural Biologist and Sceptic University of Manchester E-mail: david.c.bri...@manchester.ac.uk Internetz: http://xtaldave.posterous.com/ Twitter: @xtaldave Skype: DocDCB On 4 February 2010 13:36, Wojciech Rypniewski wojt...@ibch.poznan.pl wrote: Dear Fellow Crystallographers We are going to buy a DLS instrument to help us assess crystallizability of our proteins. I would be grateful for any opinions - either on this BB or in my mail box (in which case I'll keep them to myself, naturally). We have looked more closely at two instruments: DynaPro NanoStar from Wyatt and Zetasizer Micro V from Malvern and both seem to be rather similar in terms of parameters and price. I would appreciate user opinions as to the reliability, ease of use or any features that make a given instrument particularly suited for assessing biological samples. Many thanks, Wojtek -- -- Prof. dr hab. Wojciech Rypniewski tel: +48-61-8528503 Institute of Bioorganic Chemistry fax: +48-61-8520532 Polish Academy of Sciences e-mail: wojt...@ibch.poznan.pl Noskowskiego 12/14 www: www.man.poznan.pl/~wojtekr/ 61-704 Poznan, Poland --
Re: [ccp4bb] DLS
The Malvern is indeed very nice. The only issue I have with ours is that it doesn't do plates. This makes buffer screening a very laborious busy that is best left to graduate students. Andreas On 04/02/2010 2:22, Savvas Savvides wrote: Hi Wojtek I can easily second Dave's comment! Best Savvas Savvas Savvides Unit for Structural Biology @ L-ProBE Ghent University K.L. Ledeganckstraat 35, 9000 Ghent, Belgium Ph. +32 (0)472 928 519 http://www.LProBE.ugent.be/xray.html On 04/02/10 14:54, David Briggsdrdavidcbri...@gmail.com wrote: Hiya. My two penneth on the Malvern Zetasizer. Simple, idiot-proof operation. Software is easy and intuative. Reports are customisable to give you what information you want. Problems easy to trouble shoot IMHO. Does DLS, SLS, melting point determination. After-care support is good from Malvern. Not used the Wyatt instrument. HTH, Dave David C. Briggs PhD Father, Structural Biologist and Sceptic University of Manchester E-mail: david.c.bri...@manchester.ac.uk Internetz: http://xtaldave.posterous.com/ Twitter: @xtaldave Skype: DocDCB On 4 February 2010 13:36, Wojciech Rypniewskiwojt...@ibch.poznan.pl wrote: Dear Fellow Crystallographers We are going to buy a DLS instrument to help us assess crystallizability of our proteins. I would be grateful for any opinions - either on this BB or in my mail box (in which case I'll keep them to myself, naturally). We have looked more closely at two instruments: DynaPro NanoStar from Wyatt and Zetasizer Micro V from Malvern and both seem to be rather similar in terms of parameters and price. I would appreciate user opinions as to the reliability, ease of use or any features that make a given instrument particularly suited for assessing biological samples. Many thanks, Wojtek -- -- Prof. dr hab. Wojciech Rypniewski tel: +48-61-8528503 Institute of Bioorganic Chemistry fax: +48-61-8520532 Polish Academy of Sciences e-mail: wojt...@ibch.poznan.pl Noskowskiego 12/14 www: www.man.poznan.pl/~wojtekr/ 61-704 Poznan, Poland -- -- Andreas Förster, Research Associate Paul Freemont Xiaodong Zhang Labs Department of Biochemistry, Imperial College London http://www.msf.bio.ic.ac.uk
[ccp4bb] DLS installation Files Found
Many thanks to all who have responded with the setup files for our DynaPro DLS from Protein Solutions. Please let me know if any of you are in a similar situation and require the installation files for the data acquisition software. BestMelanie
Re: [ccp4bb] DLS options?
That being said, in-line SEC-DLS-SLS etc is a much more powerful technique, but is less straight-forward, has a larger footprint and as has been mentioned, less-inexpensive. Having an online SLS (or MALLS as people call it as well these days, multi angle laser light scattering ) is indeed nice, and clearly useful for assessing real molecular weights. Having an online DLS (which basically is more or less the same instrument but just capable to do an auto-correlation of the scattering signal over time from what I know) somehow misses the point (or do I miss the point?): a. you want to do DLS to check polydispersity of your ready-to- crystallize sample in various buffers, thus why online? 96-well format is more like it. b. if you do online DLS you need to autocorrelate the signal over a few seconds, so you loose some of the resolution in your SEC/SLS profile? I admit however that I am not sure how long you need to autocorrelate the scattered intensity to evaluate if you have a single exponential decay that would suggest a mono-disperse particle We have looked at the possibility of one instrument doing Static, On- line LS *and* DLS in a cuvette. Given that the MALLS is quite a sensitive do-not-disturb-me kind of beast, I feel better we did not do that. Tassos
[ccp4bb] DLS options?
Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option). However, it was very sensitive to dust and bubbles, and the cuvette collects dust... I've never tried the one from Precision Detectors. Any other options? Thanks Ed __ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/ http://www.bmb.leeds.ac.uk/staff/tae/ http://www.bmb.leeds.ac.uk/staff/tae/Research The doubter is a true man of science; he doubts only himself and his interpretations, but he believes in science. ~Claude Bernard
[ccp4bb] AW: [ccp4bb] DLS options?
Hi Ed, We are using a Viscotek DLS902. It seems to be robust against all types of user- and sample-unfriendliness . So were quite happy at the moment. The advantage of the system is, that you can use standard cuvettes e.g. fluorescence 3-window-cuvettes from Hellma with standard window heights. To get a nice signal we usually use black quarz cuvettes with approx. 70-100µl volume. We also use the 12µl cuvettes which are nice to have in case of lack of sample - but also hard to clean. I think theres also a model for 96well quarz-plates available è heres the URL: http://www.viscotek.com/dlsdet-sw.aspx If you want to have more info about the 902, just write me a direct email HTH gregor --- Dr. Gregor Witte Genecenter, University of Munich (LMU) AG Hopfner, Room A4.20 Feordor-Lynen-Str. 25 D-81377 Munich Tel. +49 (0)89 - 2180-76986 (Lab) Tel. +49 (0)89 - 2180-76842 (Office) Fax.+49 (0)89 - 2180-76999 mail: [EMAIL PROTECTED] web: www.lmb.uni-muenchen.de/hopfner/welcome.html Von: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Im Auftrag von Thomas Edwards Gesendet: Dienstag, 8. Juli 2008 11:21 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] DLS options? Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option). However, it was very sensitive to dust and bubbles, and the cuvette collects dust Ive never tried the one from Precision Detectors. Any other options? Thanks Ed __ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/Research http://www.bmb.leeds.ac.uk/staff/tae/ The doubter is a true man of science; he doubts only himself and his interpretations, but he believes in science. ~Claude Bernard
Re: [ccp4bb] DLS options?
I'll second the recommendation for the Malvern Zetasizer. They are rock-simple and interface with a computer through USB which makes future computer upgrades relatively simple. The are however, not cheap--oops, inexpensive. Cheers, -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL PROTECTED] Andreas Förster wrote: Hey Thomas, also consider Malvern instruments. Their Zetasizers are really sweet and work with volumes smaller than 15ul if you use the smallest cuvette. http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm The last DynaPro that I've used, half as old as the universe but equipped with a 12ul cuvette, gave me really nice results also. Key is, as you discovered, to keep the cuvette meticulously clean. I used Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml beaker, heat to 70C in the microwave with the cuvette inside, let sit for a while, rinse with water and EtOH, and dry. Only touch with gloves afterwards. The protein sample must be spun down before the experiment. Half an hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to avoid bubbles when adding the sample to the cuvette. Ah, nice data! Hope that helps. Andreas Thomas Edwards wrote: Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option). However, it was very sensitive to dust and bubbles, and the cuvette collects dust… I’ve never tried the one from Precision Detectors. Any other options? Thanks Ed __ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/http://www.bmb.leeds.ac.uk/staff/tae/ http://www.bmb.leeds.ac.uk/staff/tae/Research The doubter is a true man of science; he doubts only himself and his interpretations, but he believes in science. ~Claude Bernard
[ccp4bb] AW: [ccp4bb] DLS options?
Hmmm... we had a demo of the Malvern Zetasizer instrument here, and to be honest: It did not convince us at all (it is obviously built for non-biological particle analysis) The Viscotek is also a plugplay device, every pc with a USB is suitable. ..in the end... Viscotek and Malvern are the same company now(!) I guess the price for a DLS instrument is more or less independent of the company aren't they all approx. 30-35k euros for a single-cuvette system? Gregor -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Im Auftrag von Roger Rowlett Gesendet: Dienstag, 8. Juli 2008 17:09 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] DLS options? I'll second the recommendation for the Malvern Zetasizer. They are rock-simple and interface with a computer through USB which makes future computer upgrades relatively simple. The are however, not cheap--oops, inexpensive. Cheers, -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL PROTECTED] Andreas Förster wrote: Hey Thomas, also consider Malvern instruments. Their Zetasizers are really sweet and work with volumes smaller than 15ul if you use the smallest cuvette. http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm The last DynaPro that I've used, half as old as the universe but equipped with a 12ul cuvette, gave me really nice results also. Key is, as you discovered, to keep the cuvette meticulously clean. I used Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml beaker, heat to 70C in the microwave with the cuvette inside, let sit for a while, rinse with water and EtOH, and dry. Only touch with gloves afterwards. The protein sample must be spun down before the experiment. Half an hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to avoid bubbles when adding the sample to the cuvette. Ah, nice data! Hope that helps. Andreas Thomas Edwards wrote: Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option). However, it was very sensitive to dust and bubbles, and the cuvette collects dust Ive never tried the one from Precision Detectors. Any other options? Thanks Ed __ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/http://www.bmb.leeds.ac.uk/staff/tae/ http://www.bmb.leeds.ac.uk/staff/tae/Research The doubter is a true man of science; he doubts only himself and his interpretations, but he believes in science. ~Claude Bernard
[ccp4bb] DLS options?
Why not get an in-line, flow-cell LS detector for use with your chromatography system? We once had a great system, set up with a good SEC column, UV detection at three wavelengths on an akta, followed by SLS and DLS, and refractive index detector. The data were beautiful, as the SEC made the background very clean, and one could see easily the degree of mono/polydispersity of the SEC peak(s). It was not, however, inexpensive. Jacob Keller *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [EMAIL PROTECTED] *** - Original Message - From: Gregor Witte [EMAIL PROTECTED] To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, July 08, 2008 10:27 AM Subject: [ccp4bb] AW: [ccp4bb] DLS options? Hmmm... we had a demo of the Malvern Zetasizer instrument here, and to be honest: It did not convince us at all (it is obviously built for non-biological particle analysis) The Viscotek is also a plugplay device, every pc with a USB is suitable. ..in the end... Viscotek and Malvern are the same company now(!) I guess the price for a DLS instrument is more or less independent of the company aren't they all approx. 30-35k euros for a single-cuvette system? Gregor -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Im Auftrag von Roger Rowlett Gesendet: Dienstag, 8. Juli 2008 17:09 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] DLS options? I'll second the recommendation for the Malvern Zetasizer. They are rock-simple and interface with a computer through USB which makes future computer upgrades relatively simple. The are however, not cheap--oops, inexpensive. Cheers, -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL PROTECTED] Andreas Förster wrote: Hey Thomas, also consider Malvern instruments. Their Zetasizers are really sweet and work with volumes smaller than 15ul if you use the smallest cuvette. http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm The last DynaPro that I've used, half as old as the universe but equipped with a 12ul cuvette, gave me really nice results also. Key is, as you discovered, to keep the cuvette meticulously clean. I used Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml beaker, heat to 70C in the microwave with the cuvette inside, let sit for a while, rinse with water and EtOH, and dry. Only touch with gloves afterwards. The protein sample must be spun down before the experiment. Half an hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to avoid bubbles when adding the sample to the cuvette. Ah, nice data! Hope that helps. Andreas Thomas Edwards wrote: Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option). However, it was very sensitive to dust and bubbles, and the cuvette collects dust. I've never tried the one from Precision Detectors. Any other options? Thanks Ed __ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/http://www.bmb.leeds.ac.uk/staff/tae/ http://www.bmb.leeds.ac.uk/staff/tae/Research The doubter is a true man of science; he doubts only himself and his interpretations, but he believes in science. ~Claude Bernard
Re: [ccp4bb] DLS options?
I've used variations of the Malvern instrument at two positions now, and I have to say I've never had a problem with them. Yes, I believe it was designed to be a non-biological instrument, but I have to say it does a good job of DLS and SLS on proteins from 7-200KDa (in my experience) in a cuvette, down to ~12ul of sample. The software is fairly bulletproof, and the protein tools that give you estimates of particle axial ratios etc have proved to be pretty accurate after higher-resolution structure determination (PX, EM, SAXS) instrument itself has very few moving parts and I have never known one suffer any sort of breakdown - most problems are down to sample or cuvette cleanliness. That being said, in-line SEC-DLS-SLS etc is a much more powerful technique, but is less straight-forward, has a larger footprint and as has been mentioned, less-inexpensive. David in no way affiliated to Malvern instruments Briggs 2008/7/8 Jacob Keller [EMAIL PROTECTED]: Why not get an in-line, flow-cell LS detector for use with your chromatography system? We once had a great system, set up with a good SEC column, UV detection at three wavelengths on an akta, followed by SLS and DLS, and refractive index detector. The data were beautiful, as the SEC made the background very clean, and one could see easily the degree of mono/polydispersity of the SEC peak(s). It was not, however, inexpensive. Jacob Keller *** Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [EMAIL PROTECTED] *** - Original Message - From: Gregor Witte [EMAIL PROTECTED] To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, July 08, 2008 10:27 AM Subject: [ccp4bb] AW: [ccp4bb] DLS options? Hmmm... we had a demo of the Malvern Zetasizer instrument here, and to be honest: It did not convince us at all (it is obviously built for non-biological particle analysis) The Viscotek is also a plugplay device, every pc with a USB is suitable. ..in the end... Viscotek and Malvern are the same company now(!) I guess the price for a DLS instrument is more or less independent of the company aren't they all approx. 30-35k euros for a single-cuvette system? Gregor -Ursprüngliche Nachricht- Von: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Im Auftrag von Roger Rowlett Gesendet: Dienstag, 8. Juli 2008 17:09 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] DLS options? I'll second the recommendation for the Malvern Zetasizer. They are rock-simple and interface with a computer through USB which makes future computer upgrades relatively simple. The are however, not cheap--oops, inexpensive. Cheers, -- Roger S. Rowlett Professor Colgate University Presidential Scholar Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: [EMAIL PROTECTED] Andreas Förster wrote: Hey Thomas, also consider Malvern instruments. Their Zetasizers are really sweet and work with volumes smaller than 15ul if you use the smallest cuvette. http://www.malvern.com/LabEng/products/zetasizer/zetasizer.htm The last DynaPro that I've used, half as old as the universe but equipped with a 12ul cuvette, gave me really nice results also. Key is, as you discovered, to keep the cuvette meticulously clean. I used Pierce's RBS 35 Detergent for cleaning. Make a 2-5% dilution in a 50-ml beaker, heat to 70C in the microwave with the cuvette inside, let sit for a while, rinse with water and EtOH, and dry. Only touch with gloves afterwards. The protein sample must be spun down before the experiment. Half an hour at 13k in an Eppendorf centrifuge is sufficient. Make sure to avoid bubbles when adding the sample to the cuvette. Ah, nice data! Hope that helps. Andreas Thomas Edwards wrote: Dear BB, Sorry for the off topic question: I would like to buy a Dynamic Light Scattering system. Could people suggest which they like the best and/or which is best value? I have in the past used a Protein Solutions Dyna Pro with micro cuvette (I would like a micro cuvette option). However, it was very sensitive to dust and bubbles, and the cuvette collects dust. I've never tried the one from Precision Detectors. Any other options? Thanks Ed __ T.Edwards Ph.D. Garstang 8.53d Astbury Centre for Structural Molecular Biology University of Leeds, Leeds, LS2 9JT Telephone: 0113 343 3031 http://www.bmb.leeds.ac.uk/staff/tae/http://www.bmb.leeds.ac.uk/staff/tae/ http://www.bmb.leeds.ac.uk/staff/tae/Research The doubter is a true man of science; he doubts only himself and his interpretations, but he
Re: [ccp4bb] AW: [ccp4bb] DLS options?
We have been thinking of buying Malvern Zetasizer Nano. We were given a proper demonstration by the friendly Malvern staff, and it was satisfactory. I also had RiNA Spectroscatter 201 demonstrated to me and it seems a reasonable alternative to Malvern. I am just looking at the brochures and the Malvern instrument has a 4 mW laser while the RiNA has 100 mW. This looks like a big difference but I am not sure if the two measures are simply comparable. The measurement times during the demonstrations did not seem very different. Wojtek -- -- Prof. dr hab. Wojciech Rypniewski tel: +48-61-8528503 Institute of Bioorganic Chemistry fax: +48-61-8520532 Polish Academy of Sciences e-mail: [EMAIL PROTECTED] Noskowskiego 12/14 www: www.man.poznan.pl/~wojtekr/ 61-704 Poznan, Poland --
[ccp4bb] DLS and SEC-LS facility
Dear colleagues, I am looking for a facility where I can send samples for DLS and/or SEC-LS on a protein without long wait. Do you know of a company or academic facilty that does that. Thanks. Mike Colaneri
