[galaxy-user] Trouble Installing Galaxy on Cluster
So here is what I've installed so far. (/usr/local/galaxy) is a directory that all nodes in the cluster can see. And the web server machine can also see it.) $ls -l /usr/local/galaxy drwxrwxr-- 20 galaxy scicomp 2206 Nov 8 15:53 galaxy-dist -rwxrwxr-x 1 galaxy scicomp 1882 Nov 8 15:34 galaxy.fedora-init drwxr-xr-x 5 galaxy scicomp 67 Nov 8 10:08 galaxy_python -rw-r--r-- 1 galaxy scicomp 80 Nov 8 15:32 job_environment_setup_file drwxrwxr-- 2 galaxy scicomp 28 Nov 8 15:43 logs Here are are the changes I made to galaxy-dist/universe_wsgi.ini http://pastie.org/5351347 Here is what I put in my job_environment_setup_file: export TEMP=/scratch/galaxy source /usr/local/galaxy/galaxy_python/bin/activate The galaxy_python directory contains my virtual env based off of Python 2.7. Finally here are the contents of my galaxy.fedora-init file: http://pastie.org/5351396 (Note: I created a sym link in /etc/init.d/ to /usr/local/galaxy/galaxy.fedora-init Then ran chkconfig --add galaxy.fedora-init So I can use sudo /sbin/service galaxy.fedora-init start/stop) Here are the results from running: $sudo /sbin/service galaxy.fedora-init start http://pastie.org/5351308 So my first question is why does it appear to be using Python 2.6? Is it not using my virtual env? Am I using virtual env incorrectly? Thanks, Greg ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] [galaxy-dev] Account had be deleted ?
On Nov 7, 2012, at 5:47 AM, Diam Hsu wrote: Dear Sir or Madam, I used to use Galaxy to analyze NGS data, but these two days I could't login my Galaxy account, it also told me that my account has been marked deleted, and asked me to contact the Galaxy administrator to restore my account. Could you help me restore my accounts because I have to use Galaxy to finish my thesis. Hi, Your account was disabled because we discovered that you were using more than one account. The Galaxy Main server is a public resource and we implemented quotas to give everyone fair access to our (limited) resources. If you need more resources than we are able to make available on Galaxy Main, your needs might be best suited to a cloud or local instance of Galaxy. For more information on these, see: http://wiki.g2.bx.psu.edu/Big%20Picture/Choices If you'd like to use one of your existing accounts, please let me know which one and I'll re-enable it. --nate Thank you very much for your help!!! Best regards, Dima Hsu Graduate student, Graduate Institute of Biomedical Informatics Taipei Medical University, Taipei, Taiwan Email:b1984...@gmail.com ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] Identification of replicate outlier
Hi Ross, Thanks for the suggestions. I'm aware that this is not really a Galaxy-specific question, and I've been browsing through SeqAnswers and found a couple of suggestions using edgeR or DESeq, but nothing for Tuxedo suite. However, I have no experience with either of these tools, so I was wondering how others have approached this problem if their workflow is based on Cufflinks. In the meantime, I'll go through your suggestions and see where I get. Thanks, Dave On Thu, Nov 8, 2012 at 7:21 PM, Ross ross.laza...@gmail.com wrote: Hi Dave, This is an interesting and non-trivial question that extends well beyond Galaxy - and there's no simple solution AFAIK Defining an 'outlier' tends to boil down to subjective judgement in most real cases I've seen. EG: see http://comments.gmane.org/gmane.science.biology.informatics.conductor/40927 My 2c worth: a) confirm that all of your sample library sizes and quality score distributions are comparable with the FastQC tool. A sample with relatively low library size may indicate an upstream technical failure with (eg) RNA extraction or a flowcell lane. b) check that the number of unique alignments to the reference are similar (eg picard alignment summary metrics or even the samtools flagstat tool) c) if you can create an appropriate input matrix (read counts by exon or other contig for each sample eg), the Principal Component Analysis tool might be helpful (library size normalization is one devil that lies in the detail and it's not quite the same as MDS - see below) d) If you're an R hacker, you might find http://gettinggeneticsdone.blogspot.com.au/2012/09/deseq-vs-edger-comparison.html useful - it shows how to get MDS plots which are probably the most reliable way to identify samples that don't cluster well with the other members of their tribe On Fri, Nov 9, 2012 at 10:22 AM, Dave Corney dcor...@princeton.edu wrote: Hello list, I've been analyzing an experiment with two groups each with three replicates. My workflow was TopHat (paired end) - Cufflinks - CuffDiff. Unfortunately, there are not many significant differences identified by CuffDiff. I am wondering whether one of my replicates might be an outlier. Does anybody have a suggestion on how to search for an outlier? The quality statistics of the unprocessed data looked equally good for all samples, so I don't think that this is a problem. Thanks, Dave ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ -- Ross Lazarus MBBS MPH; Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444 http://scholar.google.com/citations?hl=enuser=UCUuEM4J ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] Teaching GALAXY
Dannon, Maybe you can, but not through me. I'm just a lowly user and biologist. You would have to contact the guy who did the installation and runs the server: Lei Yan (leiyan2...@gmail.com) I would think he would be receptive to some help, so you should mail him. LTR On 11/7/12 4:05 PM, Dannon Baker wrote: Main will complete the jobs eventually, though obviously that's not particularly useful in the context of your class today. How is your cloud instance set up? Cloud or local instances should be the best and most reliable option for teaching with and I'd definitely like to figure out why yours failed. -Dannon On Nov 7, 2012, at 3:57 PM, Larry Reiter lrei...@uthsc.edu wrote: Dannon, Thanks for getting back to me. Both the local cloud install and the main server are frozen. Well, not exactly frozen. I can still rename or delete jobs, etc. I can even switch histories and such, but I can't get it to run even simple jobs - like taking a demo file and running Fastq Groomer. I realize the load is high today on the main server, but we were able to get jobs to run right up until we tried to MAP using Bowtie for Illumina. At that point, all members of the class - and me - could not go any further (i.e. jobs are grey and never start to run). Class ended almost three hours ago. Does this help? LTR On 11/7/12 2:52 PM, Dannon Baker wrote: Can you describe more about what is broken with your cloud install? Regarding main - the server has been under heavy load today and, while it shouldn't remain frozen, might be slow to dispatch and, depending on the type, execute) jobs. -Dannon On Nov 7, 2012, at 1:12 PM, Larry Reiter lrei...@uthsc.edu wrote: Tried to teach a class today on ChipSeq analysis, but the local Cloud install is broken and then the Penn server froze on us. Is there something I should know? LTR -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page http://www.uthsc.edu/neuroscience/faculty/L_Reiter.php Reiter Citation Index http://scholar.google.com/citations?user=-kaQJ14Jhl=en Tooth Study http://tinyurl.com/88f688l ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] Teaching GALAXY
It looks like both the main server and our local install are frozen. Nothing has run since we started yesterday at about 11:30am. Anybody know why? LTR On 11/7/12 4:05 PM, Dannon Baker wrote: Main will complete the jobs eventually, though obviously that's not particularly useful in the context of your class today. How is your cloud instance set up? Cloud or local instances should be the best and most reliable option for teaching with and I'd definitely like to figure out why yours failed. -Dannon On Nov 7, 2012, at 3:57 PM, Larry Reiter lrei...@uthsc.edu wrote: Dannon, Thanks for getting back to me. Both the local cloud install and the main server are frozen. Well, not exactly frozen. I can still rename or delete jobs, etc. I can even switch histories and such, but I can't get it to run even simple jobs - like taking a demo file and running Fastq Groomer. I realize the load is high today on the main server, but we were able to get jobs to run right up until we tried to MAP using Bowtie for Illumina. At that point, all members of the class - and me - could not go any further (i.e. jobs are grey and never start to run). Class ended almost three hours ago. Does this help? LTR On 11/7/12 2:52 PM, Dannon Baker wrote: Can you describe more about what is broken with your cloud install? Regarding main - the server has been under heavy load today and, while it shouldn't remain frozen, might be slow to dispatch and, depending on the type, execute) jobs. -Dannon On Nov 7, 2012, at 1:12 PM, Larry Reiter lrei...@uthsc.edu wrote: Tried to teach a class today on ChipSeq analysis, but the local Cloud install is broken and then the Penn server froze on us. Is there something I should know? LTR -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-1160 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page http://www.uthsc.edu/neuroscience/faculty/L_Reiter.php Reiter Citation Index http://scholar.google.com/citations?user=-kaQJ14Jhl=en ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
[galaxy-user] (no subject)
Hi, I am performing online tophat on 5 different samples which I want to compare for gene expression. Is there any simple way, after the end of tophat for all of them, with which I can have an excel table with the 5 samples and their hits? Something similar to this Vevis1 Vevis2 Vevis3 Vevis4 Vevis5 uc010kuo.1 128.8503 136.60553 146.7073 91.23218 120.325 AK311687 TRA2A Homo sapiens transformer 2 alpha homolog (Drosophila) (TRA2A), mRNA. Regards Kristis Vevis, PhD Student Cell Biology UCL Institute of Ophthalmology 11-43 Bath Street London EC1V 9EL, UK 020 7608 4067 ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] tophat
It isn't normal, but this can happen during periods of extremely high load like we're currently experiencing. If you leave your jobs in the queue, they'll execute as soon as possible - don't cancel or restart your jobs as this will only move them to the back of the queue and delay completion. -Dannon On Nov 8, 2012, at 5:13 AM, Vevis, Christis christis.vevis...@ucl.ac.uk wrote: Hi all, I am trying to perform tophat for illumina from the main server of galaxy and is in queue for 24 hours…is that normal?? Regards Kristis Vevis, PhD Student Cell Biology UCL Institute of Ophthalmology 11-43 Bath Street London EC1V 9EL, UK 020 7608 4067 ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] Teaching GALAXY
Lei - It looks like you have two worker nodes, but both are currently listed as non-idle, yet have low load. My guess is that something has gone wrong with the SGE configuration causing jobs to simply wait in the queue. If you look at the contents of qconf and qstat on the admin page, does it look like there are jobs backed up and/or worker nodes processing the queue? Depending on how comfortable you are working with SGE, it might be easiest to just click restart for SGE in the admin panel and see if everything goes back to normal. -Dannon On Nov 8, 2012, at 12:43 PM, Lei Yan leiyan2...@gmail.com wrote: Dear Dannon, My name is Lei Yan, and I am a systems administrator at UTHSC. I installed a Cloud Galaxy on the Amazon EC2 about two months ago, and most functions work well at that time. http://galaxycloud.genenetwork.org/ But from the day before yesterday, jobs were hung up always. In my opinion, I didn’t find any reasons, because all look correct from Galaxy Admin page (see attachments please). Would you please help us to check it? I can give you the administrator password if you need. Thanks so much. Lei Yan leiyan2...@gmail.com UTHSC On Wed, Nov 7, 2012 at 4:07 PM, Larry Reiter lrei...@uthsc.edu wrote: Dannon, Maybe you can, but not through me. I'm just a lowly user and biologist. You would have to contact the guy who did the installation and runs the server: Lei Yan (leiyan2...@gmail.com) I would think he would be receptive to some help, so you should mail him. LTR On 11/7/12 4:05 PM, Dannon Baker wrote: Main will complete the jobs eventually, though obviously that's not particularly useful in the context of your class today. How is your cloud instance set up? Cloud or local instances should be the best and most reliable option for teaching with and I'd definitely like to figure out why yours failed. -Dannon On Nov 7, 2012, at 3:57 PM, Larry Reiter lrei...@uthsc.edu wrote: Dannon, Thanks for getting back to me. Both the local cloud install and the main server are frozen. Well, not exactly frozen. I can still rename or delete jobs, etc. I can even switch histories and such, but I can't get it to run even simple jobs - like taking a demo file and running Fastq Groomer. I realize the load is high today on the main server, but we were able to get jobs to run right up until we tried to MAP using Bowtie for Illumina. At that point, all members of the class - and me - could not go any further (i.e. jobs are grey and never start to run). Class ended almost three hours ago. Does this help? LTR On 11/7/12 2:52 PM, Dannon Baker wrote: Can you describe more about what is broken with your cloud install? Regarding main - the server has been under heavy load today and, while it shouldn't remain frozen, might be slow to dispatch and, depending on the type, execute) jobs. -Dannon On Nov 7, 2012, at 1:12 PM, Larry Reiter lrei...@uthsc.edu wrote: Tried to teach a class today on ChipSeq analysis, but the local Cloud install is broken and then the Penn server froze on us. Is there something I should know? LTR -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org . Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study -- Lawrence T. Reiter, Ph.D. Associate Professor, Department of Neurology 855 Monroe Ave., Link 415 Memphis, TN 38163 901-448-2635 (Office) 901-448-7440 (FAX) 901-448-7443 (Lab) e-mail: lrei...@uthsc.edu Reiter Faculty Page Reiter Citation Index Tooth Study 2012-11-07 13-35-01.png2012-11-07 13-36-16.png ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of
Re: [galaxy-user] (no subject)
Kristis, This data is available further downstream in an RNA-seq analysis pipeline, specifically, as output from the Cuffdiff tool. Take a look at the page for more details: https://main.g2.bx.psu.edu/rna-seq Best, J. On Nov 9, 2012, at 3:42 AM, Vevis, Christis wrote: Hi, I am performing online tophat on 5 different samples which I want to compare for gene expression. Is there any simple way, after the end of tophat for all of them, with which I can have an excel table with the 5 samples and their hits? Something similar to this Vevis1 Vevis2 Vevis3 Vevis4 Vevis5 uc010kuo.1 128.8503 136.60553 146.7073 91.23218 120.325 AK311687 TRA2A Homo sapiens transformer 2 alpha homolog (Drosophila) (TRA2A), mRNA. Regards Kristis Vevis, PhD Student Cell Biology UCL Institute of Ophthalmology 11-43 Bath Street London EC1V 9EL, UK 020 7608 4067 ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/
Re: [galaxy-user] Trouble Installing Galaxy on Cluster
I'll take a couple of stabs at this. First, make sure that /scratch/galaxy exists. There's an error at the bottom of your pastie that seems to indicate it's not there. Second, you're right about virtualenv not being used. It is likely that root is being used, which means that all of the virtualenv stuff for the galaxy user won't be available. I haven't tried this, but you could try modifying your galaxy.fedora-init to contain a call to virtualenv - that way you switch into the proper sandbox when you startup. -Scott - Original Message - So here is what I've installed so far. (/usr/local/galaxy) is a directory that all nodes in the cluster can see. And the web server machine can also see it.) $ls -l /usr/local/galaxy drwxrwxr-- 20 galaxy scicomp 2206 Nov 8 15:53 galaxy-dist -rwxrwxr-x 1 galaxy scicomp 1882 Nov 8 15:34 galaxy.fedora-init drwxr-xr-x 5 galaxy scicomp 67 Nov 8 10:08 galaxy_python -rw-r--r-- 1 galaxy scicomp 80 Nov 8 15:32 job_environment_setup_file drwxrwxr-- 2 galaxy scicomp 28 Nov 8 15:43 logs Here are are the changes I made to galaxy-dist/universe_wsgi.ini http://pastie.org/5351347 Here is what I put in my job_environment_setup_file: export TEMP=/scratch/galaxy source /usr/local/galaxy/galaxy_python/bin/activate The galaxy_python directory contains my virtual env based off of Python 2.7. Finally here are the contents of my galaxy.fedora-init file: http://pastie.org/5351396 (Note: I created a sym link in /etc/init.d/ to /usr/local/galaxy/galaxy.fedora-init Then ran chkconfig --add galaxy.fedora-init So I can use sudo /sbin/service galaxy.fedora-init start/stop) Here are the results from running: $sudo /sbin/service galaxy.fedora-init start http://pastie.org/5351308 So my first question is why does it appear to be using Python 2.6? Is it not using my virtual env? Am I using virtual env incorrectly? Thanks, Greg ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/ ___ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using reply all in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-dev To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/