Re: [base] Lowess normalisation - Block parameter

2009-02-09 Thread Louise Donnison
Great using 1 worked! Thanks From: Nicklas Nordborg To: BASE ML Sent: Sunday, 8 February, 2009 22:58:08 Subject: Re: [base] Lowess normalisation - Block parameter Louise Donnison wrote: > Hi > I have been trying to normalise with block size 48 usi

Re: [base] Lowess normalisation - Block parameter

2009-02-08 Thread Nicklas Nordborg
Louise Donnison wrote: > Hi > I have been trying to normalise with block size 48 using the Lowess > normalization plugin. It does not appear to work for me. > It gives the same result as if I use block size 0. If your array has 48 block on it this is the expected behavior. The parameter tells the

[base] Lowess normalisation - Block parameter

2009-02-06 Thread Louise Donnison
Hi I have been trying to normalise with block size 48 using the Lowess normalization plugin. It does not appear to work for me. It gives the same result as if I use block size 0. I have been looking at LowessNormalisation code. The spot spot data is ofsize 17328 split over 48 blocks. When the cod

Re: [base] lowess normalisation

2008-07-01 Thread Jari Häkkinen
The problem is now being fixed, http://base.thep.lu.se/ticket/1077. Unfortunately this problem affects all versions of BASE2 and everyone are recommended to upgrade to the future 2.7.2 (or later). The problem is localized and 2.7.2 will be released later today or at latest tomorrow. More detail

Re: [base] lowess normalisation

2008-06-30 Thread Bob MacCallum
Thanks very much Jari. Sorry I didn't have time to look deeper and figure out what was wrong. As you'll see in a few weeks we have a big project nearing release. cheers, Bob. Jari Häkkinen writes: > Hi Bob, > > We are looking into the issue. It looks like the BASE2 version of lowess > is

Re: [base] lowess normalisation

2008-06-30 Thread Jari Häkkinen
Hi Bob, We are looking into the issue. It looks like the BASE2 version of lowess is flawed. We are testing and reading publications to make sure that we make a proper fix. We already have a fix for the problem but want to make sure that we don't create new bugs. We plan to release a 2.7.2 ASAP

[base] lowess normalisation

2008-06-30 Thread Bob MacCallum
I've done some more tests on the lowess problem. Please refer from now on to our development server: http://base.vectorbase.org:8080/base2dev/ Experiment: "Male vs. female (Koutsos et al., 2007)" I ran the base1 lowess plugin and got a very different result compared to the base2 plugin. The b

[base] lowess normalisation

2008-06-27 Thread Bob MacCallum
Has anyone had problems with the Lowess analysis plugin? To me it looks like it is overcorrecting. Where a M/A plot is largely below the zero line before normalisation, it ends up largely above it afterwards. I'm using default values (including 0 for block group size). Default values were alway