[Bioc-devel] Empty DataFrame Causes SummarizedExperiment Constructor Error

2023-05-12 Thread Dario Strbenac via Bioc-devel
n `rownames<-`(`*tmp*`, value = .get_colnames_from_first_assay(assays)) : invalid rownames length What is the subtle difference? It also seems like there could be a clearer error message emitted if this is caught in the right place. ------ Dario Strbenac Universi

Re: [Bioc-devel] TCGAbiolinks fails

2023-04-28 Thread Dario Strbenac via Bioc-devel
that the package has never built successfully in Bioconductor 3.17. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] S4 Methods Documentation Convention Triggers Warnings

2023-01-27 Thread Dario Strbenac via Bioc-devel
Good day, So, is the ultimate solution to manually change everything to the format of \item{\code{show(x)}:}{ ... } ? The warnings persist, so it does not seem as though R will revert to allowing the currently-popular syntax past its check. -- Dario

[Bioc-devel] S4 Methods Documentation Convention Triggers Warnings

2022-11-26 Thread Dario Strbenac via Bioc-devel
seven years ago. What should the new convention be? Or could R developers be convinced to get rid of this check before this prototype is released? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] DataFrameList to Wide Format DataFrame

2021-12-16 Thread Dario Strbenac via Bioc-devel
ong but works: allFeatures <- unique(unlist(lapply(DFL, colnames))) DFL <- lapply(DFL, function(DF) { missingFeatures <- setdiff(allFeatures, colnames(DF)) DF[missingFeatures] <- NA DF }) DFLflattened <- do.call(rbind, DFL) Is there a one-line function for it? ----

[Bioc-devel] DataFrameList to Wide Format DataFrame

2021-12-16 Thread Dario Strbenac via Bioc-devel
measuring the largely the same set of features and patient outcome, but on completely different sets of patients in each organisation. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel

Re: [Bioc-devel] bpparam Non-deterministic Default

2021-11-26 Thread Dario Strbenac via Bioc-devel
Hello, Might it instead made possible to set an RNGseed value by specifying one to bpparam but still get the automated back-end selection, so that it could easily be set to a particular value in an R package? -- Dario Strbenac University of Sydney Camperdown

[Bioc-devel] bpparam Non-deterministic Default

2021-11-26 Thread Dario Strbenac via Bioc-devel
() which sets no RNGseed. I am wondering about the desirability of changing the RNGseed default in BiocParallel to a particular uncontroversial number, such as 12345, so that beginners get deterministic behaviour. -- Dario Strbenac University of Sydney Camperdown

[Bioc-devel] S4 Method Slow Execution if Signature Has Multiple Class Unions

2021-11-22 Thread Dario Strbenac via Bioc-devel
ge, according to top. > system.time(CrossValParams("Leave-k-Out", leave = 2)) user system elapsed 760.018 15.093 775.090 Strangely, if I rerun this code again, it works quickly the second time. > system.time(CrossValParams("Leave-k-Out", leave = 2)) user

[Bioc-devel] Delayed Assignment to S4 Slots

2021-10-13 Thread Dario Strbenac via Bioc-devel
It doesn't seem to apply to S4 slots based on my understanding of it. > r <- ResubstituteParams() > delayedAssign("r@nFeatures", nrow(measurements)) > measurements <- matrix(1:100, ncol = 10) > r@nFeatures # Still the value from empty constructor. [1] 10 20 30 40

Re: [Bioc-devel] Windows-specific Function Not Found Error

2021-10-12 Thread Dario Strbenac via Bioc-devel
. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] Windows-specific Function Not Found Error

2021-10-12 Thread Dario Strbenac via Bioc-devel
in my code to help it pass? The error doesn't appear on the two other Bioconductor servers. ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://st

Re: [Bioc-devel] VariantAnnotation Installation Compile Error

2021-05-25 Thread Dario Strbenac
Hello, The problem stemed from an .Rprofile file which was setting .libPaths with the directory path to a library of packages for the previous version of R and starting R with the --vanilla option avoided the problem. -- Dario Strbenac University of Sydney

Re: [Bioc-devel] VariantAnnotation Installation Compile Error

2021-05-24 Thread Dario Strbenac
e harmless. I will reinstall R. The extracted directory and prefix directory were the same, which might be problematic. ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org maili

Re: [Bioc-devel] VariantAnnotation Installation Compile Error

2021-05-24 Thread Dario Strbenac
Good day, No, the temporary directory has space remaining. I wonder what file it is referring to by "No such file or directory". I had an idea to reinstall Biostrings using force = TRUE, but it didn't help. ------ Dario Strbenac University of Sydney

[Bioc-devel] VariantAnnotation Installation Compile Error

2021-05-23 Thread Dario Strbenac
ACK: /dskh/biostat/software/R-4.1.0/lib/libRlapack.so ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] cannot reproduce the build error with InTAD package

2020-12-28 Thread Dario Strbenac
It looks like you are creating a MultiAssayExperiment in your vignette. Numerous Bioconductor packages relying on MultiAssayExperiment infrastructure started failing a few days ago with the release of version 1.17.3, but I don't see the breaking change explained in the News file.

Re: [Bioc-devel] Recent change in MultiAssayExperiment for inferred MAE-level colData

2020-12-28 Thread Dario Strbenac
This also happens to ClassifyR. ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] Update R package I developed which has been released by bioconductor

2020-09-20 Thread Dario Strbenac
Good day, Step 1: Follow the steps at http://bioconductor.org/developers/how-to/git/push-to-github-bioc/ -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list

Re: [Bioc-devel] BiocParallel Variable Not Found

2020-03-17 Thread Dario Strbenac
triggers the error shown in Bioconductor's daily build. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] BiocParallel Variable Not Found

2020-03-17 Thread Dario Strbenac
collected in the ellipsis, so the functional approach might still be the best in that case. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch

[Bioc-devel] BiocParallel Variable Not Found

2020-03-17 Thread Dario Strbenac
not happen on the Linux or MacOS operating systems. It happens using both R 3.6 and the upcoming version 4. The error can be reproduced running the examples of runTests function in ClassifyR. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] Use of SummerisedExperiments or MultiAssayExperiments of many many Dataframes/ nested List objects

2020-01-31 Thread Dario Strbenac
Bioconductor package S4Vectors. It's better than a data.frame and won't flood your console with output. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https

Re: [Bioc-devel] Whats the timeframe for Bioc Support updates and downtime?

2020-01-17 Thread Dario Strbenac
Good day, Could the forum have automatic saving of drafted text like some other forums? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https

Re: [Bioc-devel] Bioconductor 3.10 is released!!

2019-10-30 Thread Dario Strbenac
Good day, In the development branch, all packages are only built on Linux. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman

Re: [Bioc-devel] patch old releases? e.g. RELEASE_3_8

2019-07-12 Thread Dario Strbenac
Good day, No; anything older than the release branch at present is not modifiable. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch

Re: [Bioc-devel] how to achieve reproducibility with BiocParallel regardless of number of threads and OS (set.seed is disallowed)

2019-06-19 Thread Dario Strbenac
Good day, Should setting workers to 1 and RNGseed to a number result in a warning to the user that the seed will effectively be ignored? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc

[Bioc-devel] GAlignments Constructor Type Checking Error

2019-04-14 Thread Dario Strbenac
ructor didn't run off the edge of the PDF page in the reference manual by using \preformatted. Also, I wonder why seqnames is automatically converted into a factor Rle, but strand isn't. Couldn't strand also use .asFactorRle? ------ Dario Strbenac Univ

[Bioc-devel] GitHub Pages Vignettes

2019-03-14 Thread Dario Strbenac
ments be made explicit? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] QuasR Overwrites Base Graphics Settings

2019-03-06 Thread Dario Strbenac
uot;) qQCReport(testFile) # Fails because figure margins too large plot(1:10) # Also fails because figure margins too large The value of par("mar") is different before and after using qQCReport. Can QuasR be changed so that it does not clobber the R session's graphics parameters? -

Re: [Bioc-devel] Unable to install database package in devel version of Bioconductor 3.9

2019-02-24 Thread Dario Strbenac
Good day, You need to provide more information to get useful guidance. What version of R did you use? From the error message, it seems that it's less than 3.5.0 but it should be R Under Development. -- Dario Strbenac University of Sydney Camperdown NSW 2050

Re: [Bioc-devel] package named spdeq causes error

2019-02-21 Thread Dario Strbenac
Good day, I don't, but your software package imports agricolae which imports spdep. spdep is available from CRAN, so it's strange that the Bioconductor build server running Linux has not been able to install it. -- Dario Strbenac University of Sydney

Re: [Bioc-devel] support the stable version of R

2019-01-16 Thread Dario Strbenac
: package needs dependence on R (>= 2.10) -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] ClassifyR Check Error on Linux and MacOS Systems

2018-12-17 Thread Dario Strbenac
observed on the build servers was not clear about what the problem was. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo

[Bioc-devel] ClassifyR Check Error on Linux and MacOS Systems

2018-12-06 Thread Dario Strbenac
Good day, There is an error for ClassifyR on malbec1 and merida1 caused by a documentation example. However, it doesn't occur on tokay1. Can I get more information about which example is emitting the error on malbec1 server? -- Dario Strbenac University

Re: [Bioc-devel] Cannot access remote upstream after changing the laptop

2018-11-21 Thread Dario Strbenac
Good day, You could also copy the private key from the old computer to the new computer, if you still can use the old computer. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r

Re: [Bioc-devel] Need update access agaisnt package 'banocc'

2018-10-20 Thread Dario Strbenac
. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] Windows error "UCSC library operation failed" in package karyoploteR

2018-09-30 Thread Dario Strbenac
Good day, The import of BigWig files does not work on Windows and is documented. Execute ?BigWigFile-class and notice in the Description section: "These functions do not work on Windows.". ------ Dario Strbenac University of Sydney Camperdown NSW 2050

Re: [Bioc-devel] EXTERNAL: Fwd: PREDA problems reported in the Multiple platform build/check report for BioC 3.7

2018-08-06 Thread Dario Strbenac
Good day, Similar to you, I am awaiting the restoration of sparsediscrim which was removed on the same day as PREDA. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org

[Bioc-devel] Action for Uncompressed Data Warning

2018-06-16 Thread Dario Strbenac
asthma.RData715Kb484Kbbzip2 Should I ignore it or save it again with compression? The 231 Kb reduction in file size seems insignificant. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] BiocParallel on Windows Never Ends

2018-06-13 Thread Dario Strbenac
m Files/R/R-3.5.0/library/BiocParallel" $master [1] "127.0.0.1" $port [1] 2559 > do.call(parallel::makeCluster, cargs) # Freezes. Should I ask the question on R-devel because it doesn't appear to be specific to Bioconductor ? ---

[Bioc-devel] BiocParallel on Windows Never Ends

2018-06-12 Thread Dario Strbenac
[1] stats graphics grDevices utils datasets methods base other attached packages: [1] BiocParallel_1.14.1 loaded via a namespace (and not attached): [1] compiler_3.5.0 snow_0.4-2 parallel_3.5.0 -- Dario Strbenac University of Sydney Camperdown

Re: [Bioc-devel] Unexpected Warning About Cross-Reference Without Package Specification

2018-05-12 Thread Dario Strbenac
Good day, Thanks. I'll use the [limma] specifier to avoid the Warning from the build system. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https

Re: [Bioc-devel] Unexpected Warning About Cross-Reference Without Package Specification

2018-05-12 Thread Dario Strbenac
Good day, I created a minimalist package that demonstrates the issue and it is attached to this letter. After using R CMD build, the subsequent R CMD check process emits one warning. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] Unexpected Warning About Cross-Reference Without Package Specification

2018-05-11 Thread Dario Strbenac
Good day, limma was installed using biocLite, so it would be built before R CMD check was run. I could summarise all of the relevant information and send to R-package-devel mailing list to check if it is a bug. -- Dario Strbenac University of Sydney

Re: [Bioc-devel] Unexpected Warning About Cross-Reference Without Package Specification

2018-05-11 Thread Dario Strbenac
Good day, Indeed, it is in the Suggests component of the dependency specification. I didn't find any extra requirements for this case in the Cross-references section of Writing R Extensions, so I'm unsure of where to read about the rule. -- Dario Strbenac

Re: [Bioc-devel] BiocInstaller: next generation

2018-05-11 Thread Dario Strbenac
Alignments") to install the GenomicAlignments package in a subsequent R session, for instance. This avoids repetitive sourcing of the biocLite script from the Bioconductor server. -- Dario Strbenac University of Sydney Camperdown NSW

[Bioc-devel] Unexpected Warning About Cross-Reference Without Package Specification

2018-05-10 Thread Dario Strbenac
-linux-gnu (64-bit) * using session charset: UTF-8 -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] mcols Function Not Found for Windows Build

2018-03-15 Thread Dario Strbenac
. Is there a way to make ClassifyR guard against this problem in Windows? I don't know how to begin solving this issue. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list

Re: [Bioc-devel] BiocCheck - warning: files are over 5MB

2018-03-10 Thread Dario Strbenac
Good day, You could make use of the package named BSgenome.Celegans.UCSC.ce11. It contains the DNA sequences of all of the chromosomes of the roundworm and doesn't add any size to your package. -- Dario Strbenac University of Sydney Camperdown NSW 2050

[Bioc-devel] Numeric Operation on DataFrame

2018-01-15 Thread Dario Strbenac
3 dataTableS4 <- DataFrame(aFeature = 1:5, anotherFeature = 5:1) colMeans(dataTableS4) Error in colMeans(dataTableS4) : 'x' must be an array of at least two dimensions -- Dario Strbenac University of Sydney Camperdown NSW 2050 Aus

Re: [Bioc-devel] Emails to package maintainer bouncing back - what to do?

2017-11-19 Thread Dario Strbenac
Good day, Although the maintainer is unreachable, the original developer, Gábor Csárdi, is an active member of the R programming community. You should write to him. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] Pandoc on Build Computers

2017-11-14 Thread Dario Strbenac
earlier this week. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] exonsBy dropping genes from TxDb

2017-10-28 Thread Dario Strbenac
encode No documentation for ‘encode’ in specified packages and libraries Anyway, the transcript ID is also missing from txt. Browse[7]> grep("ENST0485971", txt) integer(0) It's hard to know what the obfuscated code of RCurl is doing. -- Dario Strbenac Un

Re: [Bioc-devel] how can I contribute to the success of great packages?

2017-10-20 Thread Dario Strbenac
simply wish to avoid that situation with genomic plotting. Indeed, I wouldn't be as cautious if I was considering csaw, for example, and noticed build system warnings close to the deadline. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] Gviz Abandonware

2017-10-20 Thread Dario Strbenac
? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] Why should Bioconductor developers re-use core classes?

2017-10-18 Thread Dario Strbenac
Good day, It might be useful to readers to have a comparison table (ticks and crosses) in the MultiAssayExperiment vignette that compares the features available in it to those available in SummarizedExperiment, to allow quicker decision making. -- Dario

[Bioc-devel] ShortRead readFasta UniProt Incorrect Import

2017-10-17 Thread Dario Strbenac
.34.2 of ShortRead which is the newest one. ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] Why should Bioconductor developers re-use core classes?

2017-10-17 Thread Dario Strbenac
. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] EXTERNAL: Fetuching Upstream Permission Denied

2017-10-11 Thread Dario Strbenac
Good day, Thanks for your help. In the end, export GIT_SSH_COMMAND='ssh -i ~/SSHkeys/digiOcean' did the trick. The write access is showing. R Wpackages/ClassifyR -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] Fetuching Upstream Permission Denied

2017-10-11 Thread Dario Strbenac
io/SSHkeys/digiOcean Copying the private key to ~/.ssh/ does not help. How can I do it? ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.

[Bioc-devel] MultiAssayExperiment Subsetting Fails if Column Data Has One Column

2017-09-12 Thread Dario Strbenac
classes) measurementsSet[1, 1, ] other attached packages: [1] S4Vectors_0.15.7BiocGenerics_0.23.1 MultiAssayExperiment_1.3.34 -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] ExperimentList Contructor Failing

2017-09-12 Thread Dario Strbenac
Good day, Whatever the problem is, it's gone with R Under Development and all packages installed from the development branch of Bioconductor. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] ExperimentList Contructor Failing

2017-09-12 Thread Dario Strbenac
;3.2.3" "0.9-8" Repository rJava "https://cran.rstudio.com/src/contrib; update with biocLite() Error: 1 package(s) out of date The same example works on another computer using Windows operating system. What's the issue with this Linux e

Re: [Bioc-devel] how to verify upstream git changes

2017-09-01 Thread Dario Strbenac
Good day, I like the idea of a commits log on the Bioconductor website. It was useful being able to see at a glance which packages have recently been changing. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] SnpSet Creation Function Prototype Not Valid R Code

2017-08-23 Thread Dario Strbenac
, object creation using new is discouraged. Perhaps SnpSet could have a proper constructor, like ExpressionSet does? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing

Re: [Bioc-devel] git and public keys

2017-08-22 Thread Dario Strbenac
Good day, Is the private key in a location other than the default SSH key folders? If so, use the ssh-add command to have the SSH agent know about it. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] Generate valid SSH keys for the bioc-git server!

2017-08-21 Thread Dario Strbenac
Good day, I filled out the form on Thursday, but can't fetch the repository. $ git fetch upstream Permission denied (publickey). fatal: Could not read from remote repository. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] Default Coverage Value

2017-07-27 Thread Dario Strbenac
verage(foo, bar, ..., NA.value=-1)?" - Tim Triche, Jr., January 2013. Might this plan be restored (with a default value of 0 for backwards compatibility)? ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] SplicingGraphs Feature Suggestion

2017-06-22 Thread Dario Strbenac
Hello, It would be convenient if the colour or the width of the edges could be customised to represent whether an edge is equally present in two experimental conditions or the degree to which it is enriched in one of them of an RNA-seq dataset. -- Dario

[Bioc-devel] pairwiseAlignment Improvements

2017-04-28 Thread Dario Strbenac
ocumented in Numeric Summary Methods? Unlike nchar and score, they are not numerical summaries of the data. It'd be nice to see this part of Biostrings thoroughly refactored with more focus on UX. -- Dario Strbenac

[Bioc-devel] ShortReadQ Serialisation Slow and Creates Large File

2017-04-26 Thread Dario Strbenac
ved in binary format, the size on disk is 2.0 GB. Is a lot of unnecessary detail saved when the object is serialised? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.

[Bioc-devel] GRangesList Conversion Fails For Unstranded Sequencing Data

2017-03-14 Thread Dario Strbenac
. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] edgeR and limma Default Offsets

2017-02-16 Thread Dario Strbenac
counts + 0.5)/(lib.size + 1) Basically, the values added to the counts and the library size ignore the library size of each sample in the voom function. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Aus

[Bioc-devel] edgeR and limma Default Offsets

2017-02-15 Thread Dario Strbenac
Good day, The cpm function in edgeR uses a default offset of 0.25 and voom in limma uses 0.5 (and provides no user modification) to calculate the base 2 logarithm of the counts per million. Might these be made consistent? -- Dario Strbenac University

[Bioc-devel] Alternative Hypothesis Specification For edgeR

2017-01-15 Thread Dario Strbenac
Good day, In a future release, could the user be allowed to specify an alternative hypothesis such as the coefficient being positive? DESeq2 provides an altHypothesis parameter for such a purpose. -- Dario Strbenac University of Sydney Camperdown NSW 2050

Re: [Bioc-devel] readGAlignments Lacks strandMode

2017-01-14 Thread Dario Strbenac
Good day, Now I know about invertStrand, I agree that it's best to keep the strandMode only for paired-end data. Indeed, it's an example at the end of the lengthy documentation of GAlignments. -- Dario Strbenac University of Sydney Camperdown NSW 2050

[Bioc-devel] GAlignments Sorting Causes C Stack Error

2017-01-08 Thread Dario Strbenac
cs_0.20.0 loaded via a namespace (and not attached): [1] lattice_0.20-34bitops_1.0-6 grid_3.3.2 zlibbioc_1.20.0 Matrix_1.2-7.1 BiocParallel_1.8.1 [7] tools_3.3.2 ------ Dario Strbenac University of Sydney Camperdown NSW 2050

[Bioc-devel] readGAlignments Lacks strandMode

2017-01-05 Thread Dario Strbenac
for readGAlignments and other similar functions in GenomicAlignments. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc

Re: [Bioc-devel] readGAlignmentPairs Fails if Used Inside mclapply Loop

2016-12-12 Thread Dario Strbenac
cores = 2 and it also resulted in an error. Each of the files has 30 to 40 million mappings, so I wouldn't expect them to be too big. I'll stick to bplapply. ------ Dario Strbenac University of Sydney Camperdown NSW 2050

[Bioc-devel] readGAlignmentPairs Fails if Used Inside mclapply Loop

2016-12-12 Thread Dario Strbenac
IRanges_2.8.1 S4Vectors_0.12.0 BiocGenerics_0.20.0 loaded via a namespace (and not attached): [1] zlibbioc_1.20.0BiocParallel_1.8.1 lattice_0.20-34tools_3.3.2 grid_3.3.2 DBI_0.5-1 Matrix_1.2-7.1 [8] rtracklayer_1.34.1 bitops_1.0-

[Bioc-devel] Implementation of vmatchPattern Indels

2016-12-04 Thread Dario Strbenac
of CRISPR genomic screens without leaving the R analysis environment, which is a new use case not existing before. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org

Re: [Bioc-devel] vmatchPattern Returns Out of Bounds Indices

2016-11-18 Thread Dario Strbenac
attern() does not support indels yet This is utilising Biostrings 2.42.0 in R 3.3.1. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/m

[Bioc-devel] vmatchPattern Returns Out of Bounds Indices

2016-11-16 Thread Dario Strbenac
to their corresponding sequence in 'x' It's rare, but still a problem, nonetheless. > table(unlist(endIndex(primerLocations)) > 75) FALSE TRUE 366225 2 This happens with Biostrings 2.42.0. ------ Dario Strbenac University of Sydney C

[Bioc-devel] Feasibility of Parallel Extraction of Matches with extractAllMatches

2016-11-16 Thread Dario Strbenac
d since it duplicates the functionality of substr? > substr(words, start(matches), end(matches)) [1] "GOAT" "MOAT" NA Also, the expected subsetting fails for MIndex objects. > class(matches) [1] "ByPos_MIndex" > length(matches) [1] 3 > length(matches[1]) [1] 3

[Bioc-devel] Subversion Log Stalled

2016-11-10 Thread Dario Strbenac
Good day, The log at http://bioconductor.org/developers/svnlog/ stopped updating two weeks ago. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https

Re: [Bioc-devel] Bioc-devel Digest, Vol 152, Issue 10

2016-11-06 Thread Dario Strbenac
for the file's existence at the beginning of the function. For example, if(file.exists(filename)) # Do fusion file import. else stop("Could not find the specified fusion file.") ------ Dario Strbenac University of Sydney Camperdown NSW 2050

Re: [Bioc-devel] chimera Attempts to Open Non-existent File

2016-11-06 Thread Dario Strbenac
. - Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] chimera Attempts to Open Non-existent File

2016-11-02 Thread Dario Strbenac
: Warning message: In file(file, "rt") : cannot open file 'Thu_Nov_3_13-46-27_2016': No such file or directory The section of code where the error occurs seems to be in the .starImport function. ------ Dario Strbenac Univ

[Bioc-devel] FlipFlop Ignores Read Strand and Requires Antiquated File Formats

2016-11-01 Thread Dario Strbenac
provide GTF and GFF3 files, which can easily be imported into R with functions provided by rtracklayer. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https

[Bioc-devel] ignoreSelf Option for findOverlaps of GenomicRanges Query

2016-10-26 Thread Dario Strbenac
to be consistent? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] NEWS files

2016-10-12 Thread Dario Strbenac
Good day, I see it, too. There's no problem. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

Re: [Bioc-devel] NEWS files

2016-10-12 Thread Dario Strbenac
s gigantic. ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] VCF Intersection Using readVcf Remarkably Slow

2016-09-27 Thread Dario Strbenac
hat is 4 GB in size when compressed. I can't imagine how long that would take. Can the code of readVcf be optimised ? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] Warning when Reading Example VCF

2016-09-27 Thread Dario Strbenac
d user. R version 3.3.1 (2016-06-21) Platform: x86_64-pc-linux-gnu (64-bit) Running under: Ubuntu 15.10 VariantAnnotation 1.18.7 ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project

Re: [Bioc-devel] Why sleuth is not in Bioconductor?

2016-09-14 Thread Dario Strbenac
nction documentation with runnable examples and a vignette. Sleuth isn't currently at the R package quality level necessary for Bioconductor. ------ Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] String Matching in Parallel

2016-09-04 Thread Dario Strbenac
calling DNAString (it's odd that vmatchPattern - for searching BSgenome objects - requires a DNAString for the pattern, rather than a DNAStringSet) or DNAStringSet ? -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

Re: [Bioc-devel] BStringSet Documentation

2016-09-02 Thread Dario Strbenac
Hello, Actually, I thought that substr unintentionally worked and perhaps they should both produce an error message. Thanks for adding the functionality for strsplit, though! -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

[Bioc-devel] BStringSet Documentation

2016-09-01 Thread Dario Strbenac
split(IDs, " ") : non-character argument I think that both of these functions shouldn't work or both should work, to be consistent. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia ___ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel

[Bioc-devel] Repitools Development Version Webpage Gone

2016-08-26 Thread Dario Strbenac
Recently, the overview webpage of the development version of Repitools has vanished. It is still listed in the build report, though. There are also some strange build errors on Linux. -- Dario Strbenac University of Sydney Camperdown NSW 2050 Australia

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