Hi Jürgen,
I'd be willing to share my datasets obtained since 1999, which I have online.
Older ones are on tape and it would be a significant effort to retrieve them,
if at all possible.
best,
Kay
Hi Phil,
Usually with something like this I use:
Data line--- bins 20
Data line--- resolution 2.80
Data line--- run 1 batch 1 to 900
Data line--- resolution run 1 high 2.80
Data line--- name run 1 project AUTOMATIC crystal DEFAULT dataset SAD
Data line--- scales constant
Data
Crushing the crustal in mother liquor and serial dilution and seeding may
help in size improvement.
On Wed, Jul 4, 2012 at 3:03 PM, REX PALMER rex.pal...@btinternet.comwrote:
Does anyone have any new tips/methods for improving crystal size once the
initial conditions have been established?
hallo every body, i face problem with protein when i make gel filtration or
concentrate my protein . i notice sever aggregation . i use only glycerol as
additives to 50 mM TRIS and 150 mM NaCL ; PH 8 . my IP is 7.8 . any suggestions.
best regards
Amr
check this article its quite useful
http://www.ncbi.nlm.nih.gov/pubmed/12711344
On Wed, Jul 4, 2012 at 4:09 PM, amro selem amro_selem2...@yahoo.com wrote:
hallo every body, i face problem with protein when i make gel filtration
or concentrate my protein . i notice sever aggregation . i use
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Hi Phil,
why do you want to use scala to merge the data? I think the default
output from scalepack is already merged, so by avoiding scala
altogether you would also avoid making extra mistakes.
Unless you want certain statistics or graphs from scala
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Dear Rex,
- - macroseeding
- - microseeding
- - further concentration by addition of precipitant to the reservoir
- - changing crystallisation technique (hanging vs. sitting drops,
crystallisation through membrane, batch crystallisation...)
- -
Dear colleagues,
Information just received here through the AFC channels: the UN general
assembly has declared that 2014 will be the international year of
crystallography.
Their statement (in French) as an attachment (sorry about including an
attachment).
F. Vellieux
IYCr-FR.pdf
Et alors, what will this mean for us?
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
Seems that toothpaste (if my translation of 'dentifrice' is correct)
figures high on the agenda!
-- Ian
On 4 July 2012 13:01, Boaz Shaanan bshaa...@exchange.bgu.ac.il wrote:
Et alors, what will this mean for us?
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion
The press release in English:
http://www.un.org/News/Press/docs/2012/ga11262.doc.htm
Boaz: perhaps the crystallography congresses and events taking place in
2014 will be attended by UN peacekeeping force members as well (with
their blue helmets) ?
F.V.
Boaz Shaanan wrote:
Et alors, what
English version: http://www.un.org/ga/search/view_doc.asp?symbol=A/66/L.51
David
On 07/04/2012 01:50 PM, Vellieux Frederic wrote:
Dear colleagues,
Information just received here through the AFC channels: the UN
general assembly has declared that 2014 will be the international year
of
And perhaps all crystallographers will have to wear those blue hats during
conferences or even during data collection sessions at syncrhotrons.
Boaz
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone:
On 07/04/12 08:03, Vellieux Frederic wrote:
The press release in English:
http://www.un.org/News/Press/docs/2012/ga11262.doc.htm
Boaz: perhaps the crystallography congresses and events taking place
in 2014 will be attended by UN peacekeeping force members as well
(with their blue helmets) ?
Do you mean we should crush the helmets and solve the structure of the
resulting powder blue coloured powder by crystallographic methods ?
Sounds like a good proposal to be put forward to the UN to help them get
rid of their used helmets !
David Schuller wrote:
On 07/04/12 08:03, Vellieux
Hi Tim,
While I use Scalepack2mtz (not from the gui) all the time, Scalepack has the
unfortunate feature that once Rsym 1.0 it gets reported as 0.0. Now that I'm
exploring higher resolution limits along the lines of the recent Karplus and
Diederichs paper (Science Vol. 336, pp. 1030-33
I use the GUI with most of the defaults , and just ask for SCALES constant
And anomalous on of course but that is by default in SCALA ..
Eleanor
On 3 Jul 2012, at 16:43, Phil Jeffrey wrote:
I'm not exactly a Scala veteran so am looking for advice as to what would be
the best way to run
Rex,
\begin{shameless self-advertizing}
Once upon a time we did a systematic study how to optimize crystal size. The
study was done with knowledge of the protein solubility (which is not normally
available, of course) and we showed that you can still do it when you do not
have this
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