[ccp4bb] VIL rich proteins

Hi all, Any of you got some experience on purifying (and crystallizing) VIL-rich proteins (actually, more particularly isoleucine rich). Tried to purify my protein, but majority (if not all) of the proteins reside in the inclusion bodies. Induction is at low temperature, overnight and

Re: [ccp4bb] off-topic: detergents for the stabilisation of water-soluble proteins

published examples? Any suggestions on the detergent type/concentration would be welcome. Thanks, Vitali -- Allan Pang Visiting Scientist G35 Joseph Priestley Building Queen Mary University of London 327 Mile End Road London, UK E1 4NS Phone number: 02078828480 Twitter: @xerophytes

Re: [ccp4bb] Etiquette on publishing if there is a crystallization report from someone else.

recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480 Twitter

Re: [ccp4bb] sulphur bridge

this protein for crystallography is not desirable . best regards Amr -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480 Twitter: @xerophytes

Re: [ccp4bb] Disulphide bonds and closed conformation

check the formation of disulphide bonds in protein. Additionally, does anybody knows another method(s) that can be used to trap a closed conformation. I look forward for your suggestions and discussions on this issue. Thanks very much! Jan -- Allan Pang PhD Student G35 Joseph Priestley

Re: [ccp4bb] Calculation of volume/size of cavity

and not expressly made on behalf of the NIAID by one of its representatives. ** -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480 Twitter: @xerophytes

[ccp4bb] 4Fe-4S

transfer. Thanks! Allan -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480 Twitter: @xerophytes

Re: [ccp4bb] Aggregated protein for crystallization

Research Scholar, Brandeis University -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480 Twitter: @xerophytes

[ccp4bb] Phenix building residues not found in input sequence file

that these amino acids are not residues found in the N-terminal region or anywhere else in the protein. Allan -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480

Re: [ccp4bb] Protein elution in Size Exclusion

://xtaldave.posterous.com/ (less sensible) Twitter: @xtaldave Skype: DocDCB On 28 August 2011 10:25, Allan Pang a.p...@qmul.ac.uk wrote: Hi there everyone, What does it mean when you have proteins eluting in almost the whole column volume of S200? I ran a gel with fractions from

Re: [ccp4bb] Protein aggregation and crystallization

concentrated and loaded on a gel filtration column Superdex-200, they elute in the void volume. But the proteins donot precipitate out !! Is it worth while to go ahead for crystallization trials?? Any other suggestion is most welcome. Thanks Anita -- Allan Pang PhD Student G35 Joseph

[ccp4bb] Protein elution in Size Exclusion

. Cheers, Allan -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480

[ccp4bb] Mosflm problem with CBF file

! Thanks, Allan -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS Phone number: 02078828480

Re: [ccp4bb] software for predicting protein solubility, stabililty and disorders

Centre for Treatment, Research and Eduction in Cancer (ACTREC) Tata Memorial Hospital, Kharghar, Navi Mumbai-410210 * # -- Allan Pang PhD Student G35 Joseph Priestley Building Queen Mary University of London London E1 4NS