[ccp4bb] Announcement of the crystallographic school in Thailand

2019-10-07 Thread Sanishvili, Ruslan
Dear Colleagues, We are delighted to announce the 2nd edition of the crystallographic school in Thailand, SEA COAST - SouthEast Asian Crystallographic Overview And Systematic Training. The workshop will take place on January 21 - 29, 2020, at the King Mongkut's University of Technology Thonburi

Re: [ccp4bb] Please share your experience about "ugly" crystals showing good diffraction

2018-07-05 Thread Sanishvili, Ruslan
Hi Anirban, It would be great if you could share the compilation of relevant responses to your request. I think many others in the community could use these examples for educational purposes. Best, Nukri Ruslan Sanishvili (Nukri), Ph.D. Macromolecular Crystallographer GM/CA@APS X-ray

Re: [ccp4bb] Oxford University Press

2018-07-04 Thread Sanishvili, Ruslan
Hi Bernhard, While I do not disagree with anything you have said, there is an error in your computation. Your results are based on the assumption of a 40-hour work week and one-month vacation in a year. I don't know about now but back then Russian scientists did not work like that. So, the

Re: [ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Sanishvili, Ruslan
Hi Annette, I do not have any experience with the particular set up you are using, but just from general considerations, how about: 1. If you have control over humidity in the room, reduce it as much as you can during "icy" data collections 2. In another e-mail you mentioned you use

Re: [ccp4bb] weird diffraction pattern

2017-07-28 Thread Sanishvili, Ruslan
Hi Chenjun Tang, I have not followed the original discussion, so my apologies if I am repeating the device already given to you. Crystallization optimization is always a good idea - nothing beats good quality crystals. In addition, you should try collecting few images, especially in the

Re: [ccp4bb] Unknown Ligand Density

2017-06-14 Thread Sanishvili, Ruslan
Difficult to guess from what is visible of the protein but is this density on a two-fold axis? Or, it could be Slimer the ghost... Cheers, Nukri Ruslan Sanishvili (Nukri), Ph.D. Macromolecular Crystallographer GM/CA@APS X-ray Science Division, ANL 9700 S. Cass Ave. Lemont, IL 60439 Tel:

[ccp4bb] Deadline approaching: 10th CCP4/APS Crystallographic School in the USA

2017-04-13 Thread Sanishvili, Ruslan
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Sanishvili, Ruslan [rsanishv...@anl.gov] Sent: Monday, March 27, 2017 3:09 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] REMINDER: 10th CCP4/APS Crystallographic School in the USA Dear Colleagues

[ccp4bb] REMINDER: 10th CCP4/APS Crystallographic School in the USA

2017-03-27 Thread Sanishvili, Ruslan
, ANL 9700 S. Cass Ave. Lemont, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 rsanishv...@anl.gov From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Sanishvili, Ruslan [rsanishv...@anl.gov] Sent: Wednesday, February 01, 2017 2:51 PM To: CCP4BB

[ccp4bb] 10th CCP4/APS Crystallographic School in the USA

2017-02-01 Thread Sanishvili, Ruslan
Dear Colleagues, We are pleased to announce the 10th annual CCP4 crystallographic school “>From data collection to structure refinement and beyond”, held at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found at

Re: [ccp4bb] A polite reminder to xia2 users

2015-11-06 Thread Sanishvili, Ruslan
Perfect segue to bring us back to the origin of this thread. Graeme was reminding the users of Xia2 that Xia2 was using other software for data analysis and that other software to be credited as well. Indeed, when we extract "Xia2 has been used for PDB files reported in X high-impact

[ccp4bb] Reminder: CCP4 crystallographic summer school in USA, at APS, June 16-23

2015-04-04 Thread Sanishvili, Ruslan
-0667 rsanishv...@anl.gov From: Sanishvili, Ruslan Sent: Wednesday, January 07, 2015 2:22 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Announcement: CCP4 crystallographic summer school in USA, at APS, June 16-23 Dear Colleagues, We are pleased to announce the eighth annual

[ccp4bb] Announcement: CCP4 crystallographic summer school in USA, at APS, June 16-23

2015-01-07 Thread Sanishvili, Ruslan
Dear Colleagues, We are pleased to announce the eighth annual CCP4 crystallographic summer school at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found at http://www.ccp4.ac.uk/schools/APS-2015/index.phphttp://www.ccp4.ac.uk/schools/APS-2014/index.php

Re: [ccp4bb] To scale or not to scale: XDS_ASCII.HKL input to POINTLESS/AIMLESS

2014-11-14 Thread Sanishvili, Ruslan
Dear Graeme, Could you elaborate on There are also some subtleties to making (b) work properly... some more? I have a feeling, from observing the beamline users, that many choose to use this option. It would be very helpful for them to know what are those subtleties and how to best make it

Re: [ccp4bb] Pilatus and Strategy wrt Radiation Damage

2014-04-30 Thread Sanishvili, Ruslan
Hi Jacob, I'll take a first crack as I am sure many will follow. It is true that with CCD detectors one has to be careful how small an oscillation range to use for a frame before read noise starts to eat into the data quality. Pilatus offers two major new features - is fast and is photon

Re: [ccp4bb] metals disapear

2014-04-30 Thread Sanishvili, Ruslan
:33 AM To: Sanishvili, Ruslan Cc: ccp4bb@jiscmail.ac.uk Subject: Re: [ccp4bb] metals disapear If metal ion will be sensitive to radiation depends on its redox chemistry and not its X-ray properties. For a metal to be affected by radiation dose it needs to be reduced by free radicals. However

[ccp4bb] Deadline is approaching: CCP4 crystallographic summer school in USA, at APS, June 24-July 2

2014-04-08 Thread Sanishvili, Ruslan
@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK Reply-To: Sanishvili, Ruslan rsanishv...@anl.govmailto:rsanishv...@anl.gov Dear Colleagues, We are pleased to announce the seventh annual CCP4 crystallographic summer school at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can

[ccp4bb] Reminder: CCP4 crystallographic summer school in USA, at APS, June 24-July 2

2014-03-17 Thread Sanishvili, Ruslan
Ruslan Sanishvili (Nukri) Macromolecular Crystallographer GM/CA@APS X-ray Science Division, ANL 9700 S. Cass Ave. Lemont, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 rsanishv...@anl.gov From: Sanishvili, Ruslan rsanishv...@anl.govmailto:rsanishv...@anl.gov Subject: [ccp4bb] CCP4

[ccp4bb] CCP4 crystallographic summer school in USA, at APS, June 24-July 2

2014-02-03 Thread Sanishvili, Ruslan
Dear Colleagues, We are pleased to announce the seventh annual CCP4 crystallographic summer school at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found at http://www.ccp4.ac.uk/schools/APS-2014/index.php Title:CCP4 crystallographic school: From data

Re: [ccp4bb] Strange Density

2012-05-15 Thread Sanishvili, Ruslan
Hi Rhis, It may have been suggested already but still... X-ray fluorescence spectra can often tell you what elements you may be dealing with. Spectra won't tell anything about binding specifics, but any extra bit of information could help. Any descent synchrotron MX beamline, equipped for

Re: [ccp4bb] CSX

2012-05-04 Thread Sanishvili, Ruslan
Hi Uma, I wish you would have shown negative densities on Fo-Fc maps as well. You protein (and the template one) may end up being a classical case to demonstrate how radiation-induced changes may lead to undesirable interpretations. Especially because it is a Cys and because it is in the active

[ccp4bb] Last call: CCP4 summer school at APS, in USA

2012-04-12 Thread Sanishvili, Ruslan
From: Sanishvili, Ruslan Sent: Tuesday, April 03, 2012 5:15 PM To: CCP4BB@JISCMAIL.AC.UK Cc: Sanishvili, Ruslan Subject: Application deadline approaching: CCP4 summer school at APS, in USA Dear Colleagues, We would like to point out that the application deadline for the 5th annual

Re: [ccp4bb] negative difference density around sulphur and oxygen atoms

2012-04-04 Thread Sanishvili, Ruslan
Hi Chris, As has been suggested already, and seems quite plausible to me, it sounds like tell-tale signs of radiation damage. To have little more substance behind this suspicion, some more experimental details could help: What was the dose accumulated during data collection? If the dose cannot

[ccp4bb] Application deadline approaching: CCP4 summer school at APS, in USA

2012-04-03 Thread Sanishvili, Ruslan
Dear Colleagues, We would like to point out that the application deadline for the 5th annual CCP4 Summer School From data collection to structure refinement and beyond is April 17, 2012. The school will take place from June 19 through June 26, 2012 at the Advanced Photon Source (APS) near

[ccp4bb] Reminder: CCP4 summer school at APS, in USA

2012-03-02 Thread Sanishvili, Ruslan
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Sanishvili, Ruslan Sent: Monday, December 19, 2011 11:37 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Annual CCP4 summer school in USA, at APS, June 19-26 Dear Colleagues, We are pleased to announce the fifth annual CCP4 summer

Re: [ccp4bb] Opinion on automation

2012-02-17 Thread Sanishvili, Ruslan
Hi Harry, Are you advertising our CCP4 Summer School coming up in June at APS? If not, then I'll shamelessly plug in: http://www.ccp4.ac.uk/schools/APS-school/ And if one visits previous years' school links, there are plenty of lectures posted. Thanks, N. Ruslan Sanishvili (Nukri), Ph.D.

[ccp4bb] Annual CCP4 summer school in USA, at APS, June 19-26

2011-12-19 Thread Sanishvili, Ruslan
Dear Colleagues, We are pleased to announce the fifth annual CCP4 summer school at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found at http://www.ccp4.ac.uk/schools/APS-2012/ Title: CCP4 school: From data collection to structure refinement and beyond

Re: [ccp4bb] dark progression of radiation damage

2011-11-23 Thread Sanishvili, Ruslan
in occasional bubbling. Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 rsanishv...@anl.gov On 11/20/2011 5:22 PM, Sanishvili, Ruslan wrote: Hi James, I don't think the comment you referenced meant to imply

Re: [ccp4bb] dark progression of radiation damage

2011-11-20 Thread Sanishvili, Ruslan
Hi James, I don't think the comment you referenced meant to imply dark progression of radiation damage. If I remember from the recent thread, it was to say that if you can only collect few (3?) shots from one crystal before it's too dead and you use 1st of these shots to devise the strategy,

Re: [ccp4bb] data processing problem with ice rings

2011-10-17 Thread Sanishvili, Ruslan
In fact, just estimating ORGX and ORGY from the first frame, using adxv or XDS-viewer, seems to do a good job. The silver lining of having ice (or any other kind) rings is that one can determine the origin rather accurately by just finding the center of these rings. Cheers, N. Ruslan

Re: [ccp4bb] small lysozyme crystals?

2011-07-28 Thread Sanishvili, Ruslan
Hi James, Not as many responses on lysozyme crystallization as I would have expected. Or may be people thought you were joking (were you?). Anyhow, higher concentrations of lyso gives smaller crystals but if you let them grow on their own, you may get few huge ones instead. What worked for me

Re: [ccp4bb] crystallization of a weird protein

2011-05-20 Thread Sanishvili, Ruslan
The milk solution is a suspension of lots of micro-crystals which form when temperature goes down. When you take the solution out, it worms up and the crystals dissolve. If you take a look at them under high magnification microscope in the cold room, chances are you'll see those crystals. I cannot

Re: [ccp4bb] how to Collecting Data from Long Unit Cell Axes ?

2011-04-05 Thread Sanishvili, Ruslan
Hi dengzq1987, You don't say how many axes are long. I will assume one. In that case, here is a compilation of what others have already recommended, with some additions: 1. Orient the crystal so that the long axis is along the rotation axis. It can be done during mounting the crystal on the

Re: [ccp4bb] how to Collecting Data from Long Unit Cell Axes ?

2011-04-05 Thread Sanishvili, Ruslan
Pictures are helpful to narrow the range of recommendations. It would be even more helpful to see the entire image, to know the orientation of the rotation axis, detector distance used, frame width, and any other information on the setup you can provide. Nukri Ruslan Sanishvili (Nukri),

Re: [ccp4bb] what to do with disordered side chains

2011-03-31 Thread Sanishvili, Ruslan
Hi All, Notwithstanding the stimulating discussion about the B-factor, I'd like to chime in with my $0.02 on the original question of to build or not to build and what are the rules and standards... and sorry for the lengthy e-mail - I was trying to respond to several comments at once. I thought

Re: [ccp4bb] Reminder: Fourth annual CCP4 summer school in USA, at APS, June 7-15

2011-03-24 Thread Sanishvili, Ruslan
Dear Colleagues, This is a reminder that the deadline for applications for the 4th annual CCP4 summer school From data collection to structure refinement and beyond is April 8. The school will take place June 7-15, 2011 at the Advanced Photon Source (APS) near Chicago. There is no

Re: [ccp4bb] disulfide bridge in red density

2011-03-06 Thread Sanishvili, Ruslan
Hi Sadaf, Most likely you are dealing with radiation damage. If that's the case, you would need to refine occupancies as Mario has already suggested. However, you may not find a double conformation. Instead, you may find that some of your sulfurs are gone. There is a ton of publications on

[ccp4bb] Reminder: Fourth annual CCP4 summer school in USA, at APS, June 7-15

2011-01-18 Thread Sanishvili, Ruslan
Dear Colleagues, This is a reminder that the on-line applications are being accepted for the 4th annual CCP4 summer school From data collection to structure refinement and beyond, which will take place early June, 2011 at the APS near Chicago. There is no registration fee for the school.

[ccp4bb] Fourth annual CCP4 summer school in USA, at APS, June 7-15

2010-12-22 Thread Sanishvili, Ruslan
Dear Colleagues, Building on the success of the past 3 years, we are pleased to announce the fourth annual CCP4 summer school at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found at http://www.ccp4.ac.uk/schools/APS-2011/index.php Title: CCP4

Re: [ccp4bb] Strange spots

2010-10-29 Thread Sanishvili, Ruslan
C'mon now! Everybody knows that frogs in real space become handsome princes in the reciprocal one... N. Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 rsanishv...@anl.gov

Re: [ccp4bb] Too many microcrystals.......

2010-10-10 Thread Sanishvili, Ruslan
Hi, What is the pI of your protein? If it happens to be anywhere near your buffer pH, then you should move away from it. Have you measured the size of you micro-crystals? You may be surprised what micro-diffraction can do for you these days. Good luck! Nukri Ruslan Sanishvili (Nukri),

Re: [ccp4bb] difficult P1 crystal

2010-09-30 Thread Sanishvili, Ruslan
Hi James, Can you, or anybody else, point me to a publication where ...not that uncommon for one or more crystal symmetry operators to collapse upon cryo-cooling... Thanks, Nukri Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439 Tel:

Re: [ccp4bb] difficult P1 crystal

2010-09-30 Thread Sanishvili, Ruslan
of Sanishvili, Ruslan Sent: Thu 30/09/2010 15:03 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] difficult P1 crystal Hi James, Can you, or anybody else, point me to a publication where ...not that uncommon for one or more crystal symmetry operators to collapse upon cryo-cooling... Thanks, Nukri

Re: [ccp4bb] Deposition of riding H

2010-09-16 Thread Sanishvili, Ruslan
sites are one of the first ones to suffer from radiation damage. And aren't the functional interpretations the ultimate goal of protein structures? Cheers, N. From: Pavel Afonine [mailto:pafon...@lbl.gov] Sent: Wed 9/15/2010 5:56 PM To: Sanishvili, Ruslan Cc

Re: [ccp4bb] Deposition of riding H

2010-09-15 Thread Sanishvili, Ruslan
Hi All, I have not read all messages in the trace so my apologies if somebody already pointed out what I have to say. There is lot of talk about how this or that software treats the riding hydrogens. What to do with the fact that however these hydrogens are treated in calculations, they are, in

Re: [ccp4bb] Third CCP4 workshop in USA, at APS, June 10-17

2010-04-07 Thread Sanishvili, Ruslan
Dear Colleagues, This is a third and final call for applications for the third annual CCP4 school: From data collection to structure refinement and beyond which will be held at Advanced Photon Source, USA. Application deadline has been extended from Friday, April 9 to Tuesday, April 12.

Re: [ccp4bb] Third CCP4 workshop in USA, at APS, June 10-17

2010-03-17 Thread Sanishvili, Ruslan
rsanishv...@anl.gov From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Sanishvili, Ruslan Sent: Wednesday, February 24, 2010 9:38 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Third CCP4 workshop in USA, at APS, June 10-17 Dear Colleagues

Re: [ccp4bb] Blue color upon X-ray exposure?

2010-03-15 Thread Sanishvili, Ruslan
Hi Todd, There are many compounds that have color centers which change color as soon as they are exposed to X-rays. Some turn, blue, some pink, some yellow etc. I'm not sure which one is the chameleon in your buffer but you can bring them separately next time and we can shoot them one by one.

[ccp4bb] Third CCP4 workshop in USA, at APS, June 10-17

2010-02-24 Thread Sanishvili, Ruslan
Dear Colleagues, Building on the success of the past 2 years, we are pleased to announce the third annual CCP4 workshop at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found at http://www.ccp4.ac.uk/schools/APS-2010/ Title: CCP4 school: From data

Re: [ccp4bb] PLEASE I NEED YOUR HELP.

2009-12-04 Thread Sanishvili, Ruslan
Best story I've heard on scamming the scammers appeared on BBC some years ago. Fortunately, it was easy to find it. Take a look at http://news.bbc.co.uk/2/hi/africa/3887493.stm Enjoy, Nukri Ruslan Sanishvili (Nukri), Ph.D. GM/CA-CAT Biosciences Division, ANL 9700 S. Cass Ave. Argonne, IL 60439

Re: [ccp4bb] coiled coil question

2009-10-04 Thread Sanishvili, Ruslan
I am not sure if this is exactly what you are asking about but 1nig in pdb sounds awfully like what you are describing. Also take a look at J Struct Funct Genomics. javascript:AL_get(this,%20'jour',%20'J%20Struct%20Funct%20Genomics.'); 2004;5(4):231-40 for more detailed description of the

Re: [ccp4bb] MAD wavelength

2009-07-16 Thread Sanishvili, Ruslan
My 2 cents: Cent #1 But they are slightly different ( may be 50 ev) in different papers. This statement itself contains the answer - always scan, but it was already suggested by many. I'd like to get to slightly different issue here. No oxidation or any other interaction in protein crystals

Re: [ccp4bb] How small is a microbeam?

2009-04-21 Thread Sanishvili, Ruslan
Hi Richard, Interesting topic raise you did... On a philosophical level, I would define a microbeam as a beam which matches in size with a microcrystal. Then question becomes what is a microcrystal? If we wanted to be purely scientific, though, we should not be measuring anything in mm

Re: [ccp4bb] How small is a microbeam?

2009-04-21 Thread Sanishvili, Ruslan
21, 2009 3:36 PM To: Sanishvili, Ruslan Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] How small is a microbeam? Sanishvili, Ruslan wrote: .. Reasons for discriminating 5-10 micron beams (minibeam) from ca 1 micron (microbeam) might have been not so much their size but what it involved

Re: [ccp4bb] How small is a microbeam?

2009-04-21 Thread Sanishvili, Ruslan
: Nave, C (Colin) [mailto:colin.n...@diamond.ac.uk] Sent: Tuesday, April 21, 2009 4:44 PM To: Sanishvili, Ruslan; CCP4BB@JISCMAIL.AC.UK Subject: RE: [ccp4bb] How small is a microbeam? Hi Yes good data with a micron size beam but, in this case, the path length was 20- 30 micron. I presume one would

[ccp4bb] CCP4 workshop in the USA, 2nd announcement

2009-03-23 Thread Sanishvili, Ruslan
[mailto:ccp...@jiscmail.ac.uk] On Behalf Of Sanishvili, Ruslan Sent: Monday, February 23, 2009 12:37 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Second CCP4 workshop in USA,1st announcement We are pleased to announce that encouraged by last year's success we are again organizing CCP4 workshop

Re: [ccp4bb] CCP4 workshop in the USA, 2nd announcement

2009-03-23 Thread Sanishvili, Ruslan
bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Sanishvili, Ruslan Sent: Monday, March 23, 2009 2:04 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] CCP4 workshop in the USA, 2nd announcement This is 2nd announcement of the CCP4 workshop entitled CCP4 school: From data collection

[ccp4bb] Second CCP4 workshop in USA,1st announcement

2009-02-23 Thread Sanishvili, Ruslan
We are pleased to announce that encouraged by last year's success we are again organizing CCP4 workshop at Advanced Photon Source (APS), Argonne National Laboratory (ANL). All details can be found here http://www.ccp4.ac.uk/schools/APS-2009/ Title CCP4 school: From data collection to structure

Re: [ccp4bb] Structural importance of ordered water?

2008-06-17 Thread Sanishvili, Ruslan
Hi Richard and Colin, There are some X-ray - visible water networks described in 0.66 A structure of Aldose Reductase (Proteins. 2004 Jun 1;55(4):792-804) and I'm sure there are plenty of similar descriptions out there. I am not an expert by any means but I think the question of water

[ccp4bb] Abstract submission deadline

2008-03-28 Thread Sanishvili, Ruslan
Dear Colleagues, This is a friendly reminder that the abstract submission deadline for IUCr Congress in Osaka, Japan is March 31st. One of the many interesting sessions is entitled Recent progress in synchrotron data collection (Session number is MS65). This session will cover new developments

Re: [ccp4bb] Color of heme containing Xtals

2008-03-12 Thread Sanishvili, Ruslan
Crystals are supposed to be red if you have oxidized iron in the heme and deep pink/scarlet if the iron is reduced. If you removed the iron (for example EDTA during purification) or substituted with something during purification or crystallization, you could loose the color. It is trivial to

[ccp4bb] CCP4 workshop in US, 2nd announcement

2008-01-15 Thread Sanishvili, Ruslan
Dear Colleagues, This is second announcement of the CCP4 workshop. WHAT CCP4 school: From data processing to structure refinement and beyond. WHERE Advanced Photon Source, Argonne National Laboratory, near Chicago, USA. ORGANIZERS CCP4, University of York, GM/CA-CAT Argonne PROGRAM

Re: [ccp4bb] CCP4 workshop in US, 2nd announcement

2008-01-15 Thread Sanishvili, Ruslan
] From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Sanishvili, Ruslan Sent: Tuesday, January 15, 2008 3:07 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] CCP4 workshop in US, 2nd announcement Dear Colleagues, This is second announcement of the CCP4 workshop

Re: [ccp4bb] a session on neurological disorders

2007-12-11 Thread Sanishvili, Ruslan
We are pleased to announce the CCP4 workshop titled CCP4 school: From data processing to structure refinement and beyond which will take place on 23-28 of May 2008 at the Advanced Photon Source (APS) near Chicago. The aim of this inaugural workshop in the USA will be to cover all areas

[ccp4bb] CCP4 workshop announcement

2007-12-11 Thread Sanishvili, Ruslan
My apologies - previous announcement went out with a wrong subject line. N We are pleased to announce the CCP4 workshop titled CCP4 school: From data processing to structure refinement and beyond which will take place on 23-28 of May 2008 at the Advanced Photon Source (APS) near Chicago.

[ccp4bb] a session on neurological disorders

2007-11-28 Thread Sanishvili, Ruslan
Dear colleague, This is to bring to your attention that there will be a session on Structural Biology in Neurological Disorders at ACA 2008. Description of the session is below. We encourage you to submit abstracts for the session and to attend while at the ACA meeting. Please note that the

Re: [ccp4bb] To bathe or not to bathe.

2007-11-26 Thread Sanishvili, Ruslan
Folks, As expected, discussion about to bathe or not to bathe are starting to expand into other aspects of data collection and processing. Along the way I saw reference to how we use our small beams on GM/CA beamlines in sector 23 of APS. Without going into discussions of advantages and

Re: [ccp4bb] DTT sensitive?

2007-11-13 Thread Sanishvili, Ruslan
This may not apply in your case but it is not uncommon for a protein to precipitate in a microcrystalline shower when put in cold. Once it worms up, the crystals dissolve and the precipitate clears up. It is easy to check under a high magnification microscope. Cheers, N. Ruslan Sanishvili

Re: [ccp4bb] SAD: Refine against anomalous data

2007-08-10 Thread Sanishvili, Ruslan
Folks, Don't take this as an argument with software people - I have a side note about structures. Back in the day when I used to solve Structural Genomics structures, we almost always knew substitution rate from masspec and Se always refined to lesser occupancy. Could it be the decay instead?

Re: [ccp4bb] Mico-focus beamline

2007-07-20 Thread Sanishvili, Ruslan
GM/CA-CAT beamlines at APS. Go to http://www.gmca.anl.gov/userprogram/applying.html to apply for beamtime. We provide 5um, 10um, 20um stable beam with fluxes of 1-2x10**11. Mini-beam (our terminology) has proven itself indispensable for number of projects covering wide range of crystal

Re: [ccp4bb] Double conformations of cysteine !

2007-07-06 Thread Sanishvili, Ruslan
It seems odd from energetic considerations that there would be a free-form amino acid when it has a choice to be involved in energetically favorable interaction. It sounds doubly odd in the case of a cystein involved in a disulfide bond. Could it be that you are observing the effects of radiation

Re: [ccp4bb] xds, saturn94 and 2theta

2007-07-06 Thread Sanishvili, Ruslan
I am not an expert in XDS but will still put my $0.02 in: Your new ORGX seems to be a reflection of the 2theta. In some other programs, origin is always defined at 2theta=0 and the angular (or linear, to that matter) offset is given as a parameter. I.e. software applies 2theta offset

Re: [ccp4bb] heavyatom soaking problem

2007-06-29 Thread Sanishvili, Ruslan
Hi, I assume Se-Met is not possible for your protein? You don't say how well (or poorly) your native crystals diffract. Increasing number of people are getting their phases by S SAD. With such affinity to high NaCl concentrations, you could also try high salt (NaCl, NaBr, RbBr etc,) soaks for

[ccp4bb] New capability at GM/CA beamlines

2007-06-15 Thread Sanishvili, Ruslan
Dear Colleagues, We are pleased to announce a new mini-beam capability at the two GM/CA-CAT undulator beamlines in Sector 23 of the APS. We have implemented an apparatus to produce a strong, stable X-ray mini-beam. The apparatus is rapidly interchangeable with our standard beam. The flux and

Re: [ccp4bb] New capability at GM/CA beamlines

2007-06-15 Thread Sanishvili, Ruslan
. Cass Ave. Argonne, IL 60439 Tel: (630)252-0665 Fax: (630)252-0667 [EMAIL PROTECTED] mailto:[EMAIL PROTECTED] From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Sanishvili, Ruslan Sent: Friday, June 15, 2007 9:26 AM To: CCP4BB