Thank you all for the overwhelming responses.
To answer your questions
The protein is monomer in solution.
Complex formation significance score shown by PISA is "Zero" and suggested
that the interface does not play any role in complex formation and seems to
be a result of crystal packing only.
The structure of the ice binding protein Maxi from Peter Davies lab (Sun et al.
2014, Science 343, 795-798) had a whole network of pentagonal waters that were
critical in holding its unusual structure (waters in the core) together.
--
Arun Malhotra
Associate Professor of Biochemistry &
There are literature shown this kind of possiblity to have dimerization
via water mediated interactions. But look whether in solution also they
could form dimer or not. How to figure out it is not non specific, it is
physiological relevant.
Cheers
SR
On Fri, Sep 27, 2019, 5:04 PM Vijaykumar
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Reg: water pentagon at dimer interface
I don’t know about the myth thing, but I remember Martha Teeter describing
pentagons of waters in crambin.
Here’s a reference:
Water Structure of a Hydrophobic Protein at Atomic Resolution: Pentagon Rings
From: CCP4 bulletin board on behalf of Ronald E.
Stenkamp
Sent: Friday, September 27, 2019 11:22 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] Reg: water pentagon at dimer interface
I don't know about the myth thing, but I remember Martha Teeter describing
pentagons
of
the National Academy of Sciences of the United States of America, 1984, 81(1),
6014-6018.
Ron
From: CCP4 bulletin board On Behalf Of Vijaykumar
Pillalamarri
Sent: Friday, September 27, 2019 4:34 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reg: water pentagon at dimer interface
Dear Community,
I
Dear All,
pentagons of water are one of the old myths from the past millennium:
everyone talks about them, nobody has seen them. How many cases do you
have in your structure, how many have you analyzed in the PDB? In other
words, anecdotal evidence is fine, but what is their statistical
: CCP4 bulletin board on behalf of Vijaykumar
Pillalamarri
Sent: 27 September 2019 12:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reg: water pentagon at dimer interface
Dear Community,
I solved the structure of a protein from vibrio. There are two molecules in the
asymmetric unit
Pentagons of water are quite common in ordered water structure. Is it isolated or do the waters H-bond to other waters?The dimer interface looks, if I may say, a bit borderline to me. How does it fare in Pisa? Best wishes. On 27 Sep 2019 12:33, Vijaykumar Pillalamarri wrote:Dear Community,I
