: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] biomolecular NMR for IDPs
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Hello Sorin,
1. The cost of getting NMR data on the IDPs you propose depends upon the
expression levels of the protein/s as you will need to label with 15N and 13
y
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To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] biomolecular NMR for IDPs
[External Email From]: owner-ccp...@jiscmail.ac.uk
Hello Sorin,
1. The cost of getting NMR dat
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2021 8:20 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] biomolecular NMR for IDPs
[External Email From]: owner-ccp...@jiscmail.ac.uk
Hello Sorin,
1. The cost of getting NMR data on the IDPs you propose depends upon the
expression levels of the protein/s as you will need to l
Hi
Just my two ha’porth.
I’m currently involved in a project where my collaborators are investigating
the interactions between protein pairs (both hetero and homo) - they
specifically asked me _not_ to give them any models from ensembles (actually,
they said “no NMR structures because they
Hello Sorin,
1. The cost of getting NMR data on the IDPs you propose depends upon the
expression levels of the protein/s as you will need to label with 15N and 13C -
and depending upon your overall yields per liter of E.coli culture, this can
add up. In addition you will need to run triple
: Re: [ccp4bb] biomolecular NMR for IDPs
Hi James,
Could you shed some light on the costs involved? This has been done also in the
case of the 2 proteins that I am interested in - however the data is not
publically available and the authors of the articles are reluctant to share the
data, which
Hi James,
Could you shed some light on the costs involved? This has been done also in
the case of the 2 proteins that I am interested in - however the data is
not publically available and the authors of the articles are reluctant to
share the data, which I could understand.
Many thanks!
On Sun,
Hello Jon,
Indeed - in the case of NMR, as far as I understand, we can't talk about
resolution (that is why I used quotes). What I need here is a structure (or
ensemble of structures) to which I could hope to dock ligands with some
level of accuracy - something that SAXS could not provide.
On
It is possible to get an ensemble for an intrinsically disordered segment from
NMR. We did this in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008819/
Best wishes
James
On Aug 15, 2021, at 14:48, Jon Cooper
<488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote:
Hello, my numpty-level
Hello, my numpty-level understanding is that being intrinsically disorder and
giving high-resolution structural data are mutually exclusive. I will re-read
your e-mails. Hope this helps. Cheers, Jon.C.
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Original Message
On 15 Aug 2021, 09:16, Sorin
Hello Ethan,
Thank you for the suggestions. I should have mentioned in my initial post
that my intention is to first conduct a high throughput virtual screening
on these proteins, thus I would need high "resolution" of the structures
which SAXS could not provide, as far as I understand.
SAXS/SAS
It is possible that you could address some of your questions
more quickly and much more cheaply by small-angle scattering,
either light (SAS) or X-ray (SAXS).
I would suggest looking into those avenues first.
If you have well behaved (i.e. non-aggregating) purified proein
and access to
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