will work fine but leave overnight.
psp
On Tue, Jun 2, 2009 at 5:36 PM, Jerry McCully
wrote:
> Dear All:
>
> Recent we expressed one protein using mammalian cells but this
> protein was highly glycosylated (30% of the total molecular weight).
>
> We want to remove some carbohydrate
Hi Peter,
You first want to reduce the protein concentration to avoid rapid
precipitation upon adding crystallization solutions. Try concentrations
of 5 - 10 mg/mL instead of 15 mg/mL. Next, consider changing the NaCl
concentration. Salt can act as a co-precipitant, and lowering its
concentrati
Hi all,
we a one weird problem with one of our machines.
Upon launching ccp4i, we get an error message in the shell window:
[us...@pahto ~]$ ccp4i
bad marshal data
Then, once the interface finally starts after quite a while a new window
opens with the following message. None of the previous proje
Hi all,
we a one weird problem with one of our machines.
Upon launching ccp4i, we get an error message in the shell window:
[us...@pahto ~]$ ccp4i
bad marshal data
Then, once the interface finally starts after quite a while a new window
opens with the following message. None of the previous proje
Hi all,
we a one weird problem with one of our machines.
Upon launching ccp4i, we get an error message in the shell window:
[us...@pahto ~]$ ccp4i
bad marshal data
Then, once the interface finally starts after quite a while a new window
opens with the following message. None of the previous proje
Dear All:
Recent we expressed one protein using mammalian cells but this protein
was highly glycosylated (30% of the total molecular weight).
We want to remove some carbohydrate by enzymatic digestion for the
convenience of crystallization. However, this protein tends to aggregat
For something that large, would it be easier and cheaper to
make E. coli do it? Say, fuse your sequence to ubiquitin?
Phoebe
Original message
>Date: Mon, 1 Jun 2009 12:09:00 -0400
>From: Pius Padayatti
>Subject: Re: [ccp4bb] Request recommendation for the peptide
synthesis company
Hi all,
I would like to compile a summary of what sorts of software that labs,
departments, or individual crystallographers are using to track their
experimental work. Of course, whatever responses I receive will be
summarized and reposted to the ccp4bb. Hopefully, this can benefit us
all.
More
I need to model in tetrachloroaurate molecules into a structure, from our
heavy atom soak of Potassium tetrachloroaurate.
I created a pdb file by using chemdraw and exporting as an *.sdf and opening
in pymol and saving as a *.pdb (attached)
I let refmac create a library file (also attached)
==
Beamtime available at CHESS, September 16-November 10, 2009
==
The CHESS/MacCHESS facility, located at Cornell University in Ithaca,
NY, invites macromolecular crystallograp
On Tue, Jun 02, 2009 at 12:57:59PM -0400, Ed Pozharski wrote:
> I've seen this before in some structures and have verified in those
> cases that correction of the bulk solvent mask fixes the negative
> density.
> ...
> It was quite convincing that it's indeed empty cavities large enough
> to be fi
Hello all
I am working on small protein-protein complex of 10 kDa each component.Among
these complex one of the partner known to be highly
flexible but in complex they are suppose to be well structured and from a
tight complex . I am trying to crystallise this complex. When i add the
protein in
I've seen this before in some structures and have verified in those
cases that correction of the bulk solvent mask fixes the negative
density. Those were refined with CNS, and I had a script running
model_mask.inp, followed by mama to remove the internal cavities (invert
it, do island_erase, inver
POSTDOCTORAL FELLOW POSITION, UNIV. OF PITTSBURGH School of Medicine
A postdoctoral fellow position is available immediately in the Zhang
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NIH fund
Sorry found it:
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1853337
... and it's Brian Matthews not Mathews.
-- Ian
> -Original Message-
> From: owner-ccp...@jiscmail.ac.uk [mailto:owner-ccp...@jiscmail.ac.uk]
On
> Behalf Of Ian Tickle
> Sent: 02 June 2009 10:35
> To: Ana-M
The other possibility of course is that the data is good, that this is
an accurate experimental result and there really is a void, or at least
a cavity where the mean bulk density is lower than in bulk water. One
way to test the void theory would be to fill the cavity with O atoms of
zero (or very
A three-year PhD studentship at the interface of molecular simulation
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Synchrotron SOLEIL, 20 km from Paris.
Applicants should have a masters degree in physics
Dear Ana-Maria,
in my experience, mask bulk solvent artifacts can only occur at narrow
channels, where the mask radius is too big to define the channel as
belonging to the bulk solvent region, leaving it "empty" and thus
resulting in positive (!) difference density. Changing from simple
s
A postdoc position is available in the group of Molecular Structural
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Dear BB,
I'm refining a structure which presents a big hydrophobic cavity where a
strong residual difference negative density can be seen. I presume that
this is an effect of not having a proper solvent mask determined and I
was wondering if there is a way to provide a better mask description
In agreement with the previous posts and to avoid any confusion with
respect to Coot. Here is a previous post about this issue:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0901&L=CCP4BB&P=R94235
Bernhard
Dear All,
I see that the UK is catching up and the NVIDIA Geforce 3D Vision Bundle
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