Dear all, please note:
*AnoXtal: a new platform dedicated to protein crystallization under
anaerobic conditions.*
**
**
**
The Metalloproteins Group of the Institut de Biologie Structurale will
receive demands for anaerobic protein crystallization, from both the
academic and private
Rhys,
We have solved several structures in our lab using bicelles (all beta-barrels).
It is generally the case that we want to minimize excess detergent for
crystallization and it is the same for bicelles. At least for our cases, we
have not done anything extra to remove excess detergent
Hi everyone,
Thank you for your responses I will keep on with optimisation!
Cameron
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of CAMERON
FYFE
Sent: 11 December 2013 14:57
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystallisation
Hi all;
I have crystal of membrane protein (110 kDa) in several different
conditions. I optimized several of them and tested it for their
diffraction. It does not goes behind 10A at CLS and SSRL.
The protein is purified by two steps nickel and gel filtration. The
crystal grow very fast and reach
Happy Holidays.
Please review the position specifications and advise if someone in your
network may have an interest in finding about more about this exciting
Crystallography opportunity with DNS.
Opportunity: DNS offers competitive salaries and a great benefits package
including
Dear all,
I have collected ~160 degrees of data on a new crystal form of a protein which
has already been solved. Data was processed with XDS and reindex, scaled and
truncated with Aimless. Both XDS and Pointless suggested a Laue group of
P6/mmm with a possible space group of P6122 or P6522.
Hi Rhys,
I hope you are well. The JOVE article for bicelles is nice, plz check the
video. There is a table in the text section showing some structures that
originated from crystals grown at 4-7 % bicelle conc. using not only the
DMPC/CHAPSO mixture... The other parameter that everyone tries is
2.8 is not a terrible resolution to try out Buccaneer or Parrot. Your terrible
R-factor might be due to a shift in residues perhaps ? Your After-building map
does not show much of a side chain density to judge if you are in frame or off.
But the elongated helix is in my eyes convincing enough.
places still available register on the day
BCA Winter Meeting 2013
Title: New X-ray Developments and Macromolecular Structures in the Bragg
Centenary Year.
Venue: King’s College London (New Hunt's House, Guy's Campus, London Bridge,
SE1 1UL).
Date: 16th December 2013.
