[ccp4bb] Postdoc position in structural enzymology in Cambridge, UK

y specific questions you might have. Cheers, Stephen -- Prof. Stephen Graham School of Biological Sciences Theme Leader: Infection and Immunity Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP http://www.atomicvir

Re: [ccp4bb] Bond between Cys S and Met methyl group?

et and Cys side chains, plus perhaps a close contact of the Met with Cys in one of the conformations. Sadly : ( Thanks all on and off-list for the good ideas! Stephen From: herman.schreu...@sanofi.com Sent: 17 July 2019 15:18 To: Stephen Grah

[ccp4bb] Bond between Cys S and Met methyl group?

Thanks, Stephen -- Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP http://www.path.cam.ac.uk/research/investigators/graham/

[ccp4bb] Risk assessment for heavy atom soaking - examples?

ng to share the risk assessments they have in their institute/company so that I can seek inspiration and make sure I'm keeping up with best practice. Many thanks, Stephen -- Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Te

[ccp4bb] Postdoc in Cambridge on protein:protein interactions in eukaryotic membrane trafficking

/ and applications close on April 27th (only via the website please, it makes the HR people feel unloved otherwise). Cheers, Stephen Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP Ph:

[ccp4bb] Postdoc in Cambridge on Virus:Host interactions

cover letter that you’re applying for Position 3. Applications close on Tuesday, so there’s no time to waste! Cheers, Stephen Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP Ph: 01223

Re: [ccp4bb] Off Topic: Isothermal Titration Calorimetry (ITC)

           * > * Tel.: +351.21.446.97.61                      * > * Fax:  +351.21.443.36.44                      * > *                                              * > * E-mail: jbr...@itqb.unl.pt                   * > * URL: http://mx.itqb.unl.pt                   * >

Re: [ccp4bb] : Re: [ccp4bb] unusual bond lengths in PRODRG cif file

llographic Data Centre > 12 Union Rd., Cambridge CB2 1EZ, UK > T:  +44-(0)1223-762532 > F:  +44-(0)1223-336033 > > > > LEGAL NOTICE > Unless expressly stated otherwise, information contained in this > message is confidential. If this message is not intended for you, > please in

Re: [ccp4bb] non-waters among structured solvent atoms

ter" assemblies matching the geometry of coordinated cations has >> previously been described. But none of my google searches has turned >> up the references I was looking for. Could someone point me in the >> right direction, please? >> Many thanks, >> Wolfram Temp

Re: [ccp4bb] Per-residue RMSD for multiple structures?

per-residue deviations by doing SSM superposition in Coot, > but that doesn't get me to an RMSD for all four structures jointly. > >        Ethan > > > -- > Ethan A Merritt > Biomolecular Structure Center > University of Washington, Seattle 98195-7742 > -- Dr

Re: [ccp4bb] Anions in protein structures

ely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and then > delete it from your system. > -- Dr Stephen Graham 1851 Research Fellow Camb

Re: [ccp4bb] Crystallization of lysine and arginine rich proteins

s > binding partners and could not succeed.  I think any compound that binds to > the lysine/arginine side chains might affect the crystallization process > thereby reducing the internal flexibility of protein.  Can anybody suggest > some effective strategy for the crystallization? > > &

Re: [ccp4bb] off topic; alignment keeping upper and lower case

ggestions much appreciated. >       regards >        Martyn > > Martyn F. Symmons > Department of Pathology > University of Cambridge > > 'Gun fhaillin 'san fhairge..' > (said of Griomasaigh boats) > -- Dr Stephen Graham Division o

Re: [ccp4bb] European IPTG suppliers

van Raaij > Dpto de Bioquímica, Facultad de Farmacia > Universidad de Santiago > 15782 Santiago de Compostela > Spain > http://web.usc.es/~vanraaij/ > -- Dr Stephen Graham Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] My cysteines

nsity in the region. > > James > > > > > -- Dr Stephen Graham Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] long term data backup

ane Structural and Functional Biology Group > L2-007, Lonsdale Building > University of Limerick, Ireland > T: 353 (0)61 202302 > F: 353 (0)61 234329 > -- Dr Stephen Graham Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] difficult MAD dataset

(so long as you have reasonably redundant data). So, re-process with 'scale anomalous' and then run autoSHARP in all sub-spacegroups of P212121 - as Tassos will tell you this works a treat! Cheers, Stephen -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] Google marks CCP4 web site as a potential security threat

9 >> France >> Tel : +33(0) 491 82 55 93 >> Fax: +33(0) 491 26 67 20 >> e-mail: miguel.ortiz-lombar...@afmb.univ-mrs.fr >> Web: http://www.pangea.org/mol/spip.php?rubrique2 >> >> >> -- >> This message has been scanned for viruses and >> dangerous con

Re: [ccp4bb] problem of crystallization

recipitation. The rest of wells are pretty clear still. Is there any > suggestion for reducing protein solubility or increasing the chance of > getting crystals? > > Thanks for your time, > Jennifer -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellco

Re: [ccp4bb] discontinuous data wedges in XDS

van den Berg > University of Massachusetts Medical School > Program in Molecular Medicine > Biotech II, 373 Plantation Street, Suite 115 > Worcester MA 01605 > Phone: 508 856 1201 (office); 508 856 1211 (lab) > e-mail: [EMAIL PROTECTED] > http://www.umassmed.edu/pmm/facu

Re: [ccp4bb] multiple sequence alignment from multiple pairwise structural alignments

t; "Structure Comparison" -> "Match->Align" Much easier than hand-editing the alignment! Thanks again, Stephen On 3/4/08, Stephen Graham <[EMAIL PROTECTED]> wrote: > Hi all, > > I would like to generate a structure-based multiple sequence alignment > using 4

[ccp4bb] multiple sequence alignment from multiple pairwise structural alignments

t a number of pairwise sequence alignments) and calculate the 'best' multiple sequence alignment? Please note that there is absolutely no sequence conservation between these structures, making standard sequence-based alignment tools pretty useless. Thanks, Stephen -- Dr Stephen Gra

Re: [ccp4bb] anomalous signal of Mn and Ca ions

the anomalous signal from Mn or Ca? > > Any ideas and suggestions are greatly appreciated! > > Sun Tang > > > Never miss a thing. Make Yahoo your homepage. > > -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] Vary b-factors from a plane/point.

er & Crystallographer > http://personalpages.manchester.ac.uk/staff/David.C.Briggs/ > < BROKEN! > AIM ID: dbassophile > -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive

Re: [ccp4bb] extra density on Cysteine

gapore 138670 > Fax: +65 6722 2916 > Phone: +65 6722 2980 > -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] highly soluble proteins

t;salting out" using dialysis, and also adding > > ligands/inhibitors. The protein is in 50 mM NaCl plus 50 mM buffer. > > > > I welcome thoughts and suggestions on crystallization ideas, > > publications, etc. > > > > Thank you > > > > Yvonne > > > -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] Stereo grpahics and Virtual Reality

(http://www.wiili.org/Wiimote) and have already been used for controlling industrial robots (http://www.youtube.com/watch?v=0qEotHQgUsg). Stephen -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN

Re: [ccp4bb] Differentiating bound Mn & Ca.

dbriggs.talktalk.net iChat AIM ID: DBassophile --- Anyone who is capable of getting themselves made President should on no account be allowed to do the job. - Douglas Adams -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome T

Re: [ccp4bb] [COOT] [ccp4bb] Colour surface by sequence conservation

GRASP - modify the B-factor column in >>>>> the >>>>> PDB file to represent the percentage conservation and then colour the >>>>> surface by B-factor. I know ESPRIPT will make the modified file, but I'm >>>>> having trouble generating t