Dick Dickerson tried to do the same thing at Caltech around the same time.
The major problem with cooling equipment was that the Picker goniometer
had lots of metal in it, and each of the metal pieces cooled and
contracted differently, so the alignment was always off. Nice idea, but
not useful. Tha
I've been in that cold room / hutch. I never heard of it being flushed
with LN2. I think that is just to make the room sound cooler.
Jim
On Thu, 19 Jun 2008, Mischa Machius wrote:
Sadly, I have never seen the room being used. Perhaps one of the 'older'
Martinsrieder on the forum has seen it
Sadly, I have never seen the room being used. Perhaps one of the
'older' Martinsrieder on the forum has seen it. MM
On Jun 19, 2008, at 12:11 PM, Klaus Futterer wrote:
"... room that was lined with insulated steel walls and that could
be flushed with liquid nitrogen."
I'm trying to pictur
"... room that was lined with insulated steel walls and that could be
flushed with liquid nitrogen."
I'm trying to picture this ... did you guys have some kind of LN2-
proof SCUBA diving equipment to work in there?
Klaus
---
Ha, everyone seems to be bragging about how far back cryo-
crystallography really goes. In that vain, I'd like to mention that,
in Martinsried, we had a room that was lined with insulated steel
walls and that could be flushed with liquid nitrogen. It was requested
(demanded, really...) by Ro
Well everyone, talking of early applications of cryocooling to X-ray
crystallography, what about Sten Samson's marvellous helium cryostat
which was operational at Caltech since the end of the 1970s and used to
reach temperatures around 20 K routinely , see for example:
Proc Natl Acad Sci U S A
e the
>> contribution from thermal motion.
>>
>> -- Ian
>>
>>> -Original Message-
>>> From: [EMAIL PROTECTED]
>>> [mailto:[EMAIL PROTECTED] On Behalf Of harry powell
>>> Sent: 19 June 2008 14:05
>>> To: Remy Loris
>>
if you're
into
valence density deformation maps, again in order the minimise the
contribution from thermal motion.
-- Ian
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of harry powell
Sent: 19 June 2008 14:05
To: Remy Loris
Cc: CCP4BB@JISCMAIL.A
14:05
> To: Remy Loris
> Cc: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] is it Ok to freeze
>
> Hi
>
> Without wishing to start an argument, I've been checking with
> some of my colleagues who are chemical crystallographers -
> the reply I get is that, for routine stru
This fellow below is presumably and Indian,
writing in English at a German University,
a very confused new generation researcher, indeed.
Maybe this will help:
S.Jayashankar
(Ein wenig verwirrter Forscher der neuen Generation)
Forschungsstudent
Institut fuer Biophysikalische Chemie
Medizinische Sc
Hi
Without wishing to start an argument, I've been checking with some of
my colleagues who are chemical crystallographers - the reply I get is
that, for routine structural analysis, "pretty well all datasets are
collected at 100K unless the crystals fall apart at low T, or if the
cryostre
Typically crystals of small organic compounds do not require freezing as
there are no solvent channels. They do in general not suffer from
radiation damage at room temperature the way protein crystals do.
Occasionally they are mounted in a capillary instead of simply glueing
them to a goniomete
Hi
I just noticed I scrambled that first paragraph. I didn't intend to
imply you should be able to see your referees (or their criteria) at
least some of the time. It should have read something more like-
yes, both of those. Plus "freezing out" multiple conformations so
that you can mode
tion in B
factors, reduction in radiation damage, anything else?
Colin
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf
Of harry powell
Sent: 19 June 2008 10:12
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] is it Ok to freeze
Hi
If you mean organic
rd [mailto:[EMAIL PROTECTED] On
Behalf Of harry powell
Sent: 19 June 2008 10:12
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] is it Ok to freeze
Hi
If you mean organic small molecules, then the opinion for the
last 15 years at least is pro
Hi
If you mean organic small molecules, then the opinion for the last 15
years at least is probably "yes, unless you know you'll have a phase
change".
Most small molecule crystals don't have the same problems with
needing cryoprotectants as macromolecules, due in large part to not
havin
Dear Scientists and Friends,
I am not sure, whether organic crystals need to be in cryo stream
necessarily during data collection from an in house
xray machine .
How most of the organic crystals have been solved mostly?
--
S.Jayashankar
(A bit confused new generation researcher).
Research
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