ag, 14. November 2017 00:45
> *An:* CCP4BB@JISCMAIL.AC.UK
> *Betreff:* [EXTERNAL] [ccp4bb] High R/Rfree
>
>
>
> Hello All,
>
>
>
> I am currently working on the structure of a DNA protein complex. The
> data has been processed in space group P1 (53.042 59.527 78.52
problem there like streaks,
ice rings etc.
Best,
Herman
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Radhika
Singh
Gesendet: Dienstag, 14. November 2017 00:45
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [EXTERNAL] [ccp4bb] High R/Rfree
Hello All,
I am currently working on the
Hi Radhika,
R-factor value is almost useless unless you know the resolution (which you
did not tell us): 26% is ok for 3A resolution and is nonsense for 1A, for
example. The Rfree-Rwork gap is obviously large, suggesting sub-optimal
refinement strategy. Try optimizing weights, let program update
(
Hello All,
I am currently working on the structure of a DNA protein complex. The data
has been processed in space group P1 (53.042 59.527 78.526 105.24
98.03 106.99 P 1, Rpim 11.7%). At this stage I have almost 85% model is
complete but my R/Rfree is stuck as 26%/34%.
I have some concerns a
Paul Steven Miller (PhD)
> >> Postdoctoral Researcher
> >> University of Oxford
> >> Wellcome Trust Centre for Human Genetics
> >> Division of Structural Biology
> >> Roosevelt Drive
> >> Oxford
> >> OX3 7BN
> >>
> >&
iso whether anisotropy can
> cause this problem and whether there is any way around it.
> > >>
> > >> Cheers, Paul
> > >>
> > >> Paul Steven Miller (PhD)
> > >> Postdoctoral Researcher
> > >> University of Oxford
> > &g
und it.
> >>
> >> Cheers, Paul
> >>
> >> Paul Steven Miller (PhD)
> >> Postdoctoral Researcher
> >> University of Oxford
> >> Wellcome Trust Centre for Human Genetics
> >> Division of Structural Biology
> >> R
; >> Postdoctoral Researcher
> >> University of Oxford
> >> Wellcome Trust Centre for Human Genetics
> >> Division of Structural Biology
> >> Roosevelt Drive
> >> Oxford
> >> OX3 7BN
> >>
> >>
> >> Original mes
ty of Oxford
>> Wellcome Trust Centre for Human Genetics
>> Division of Structural Biology
>> Roosevelt Drive
>> Oxford
>> OX3 7BN
>>
>>
>> Original message
>>
>>> Date: Fri, 13 Oct 2017 10:30:22 +0200
>>> From: CCP4
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Gianluca
Cioci
Gesendet: Freitag, 13. Oktober 2017 10:30
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [EXTERNAL] [ccp4bb] High R/Rfree after MR
Dear All,
I am trying to refine a structure at 3.3A. Model has 60% identity to the
target
al message
Date: Fri, 13 Oct 2017 10:30:22 +0200
From: CCP4 bulletin board (on behalf of Gianluca Cioci
)
Subject: [ccp4bb] High R/Rfree after MR
To: CCP4BB@JISCMAIL.AC.UK
Dear All,
I am trying to refine a structure at 3.3A. Model has
60% identity to the target. Maps look OK (for 3.
n Miller (PhD)
>Postdoctoral Researcher
>University of Oxford
>Wellcome Trust Centre for Human Genetics
>Division of Structural Biology
>Roosevelt Drive
>Oxford
>OX3 7BN
>
>
> Original message
>>Date: Fri, 13 Oct 2017 10:30:22 +0200
>>From: CCP4 bulleti
University of Oxford
Wellcome Trust Centre for Human Genetics
Division of Structural Biology
Roosevelt Drive
Oxford
OX3 7BN
Original message
>Date: Fri, 13 Oct 2017 10:30:22 +0200
>From: CCP4 bulletin board (on behalf of Gianluca Cioci
>)
>Subject: [ccp4bb] High R/Rfree after MR
Dear All,
I am trying to refine a structure at 3.3A. Model has 60% identity to the
target. Maps look OK (for 3.3A) and rebuilding in Coot is relatively
straightforward. However, after some rebuilding cycles the R factors are
stuck at 0.37/0.39 (REFMAC).
XTRIAGE tells me that everything is normal (
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