Re: [ccp4bb] Off-topic: Fungal growth in robot trays
Dear All: Thank you for your responses. Here is a summary of suggested fixes: 1. Cleaning the supply carboy and lines with bleach and flushing thoroughly with DD water afterwards 2. Adding 0.02% sodium azide to the protein 3. Adding 0.02% azide to commercial screens 4. Adding 0.02% azide to the water used for washing 5. Using fresh screens and storing them at low temperatures (4 or 12 degree C) 6. Manually dispensing the reservoir solution using a multi-channel pipette 7. Using a Mosquito robot (it uses fresh needles each time) Best, Vish From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Viswanathan Chandrasekaran Sent: Friday, March 08, 2013 4:24 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Off-topic: Fungal growth in robot trays Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don't have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish
Re: [ccp4bb] Off-topic: Fungal growth in robot trays
8. Empty the carboy and fill the supply lines and wash stations with 20% ethanol at the end of the day. (Requires decontamination in this case) Best regards, Dmitry On 2013-03-13, at 10:04 PM, Viswanathan Chandrasekaran v...@biochem.utah.edu wrote: Dear All: Thank you for your responses. Here is a summary of suggested fixes: 1. Cleaning the supply carboy and lines with bleach and flushing thoroughly with DD water afterwards 2. Adding 0.02% sodium azide to the protein 3. Adding 0.02% azide to commercial screens 4. Adding 0.02% azide to the water used for washing 5. Using fresh screens and storing them at low temperatures (4 or 12 degree C) 6. Manually dispensing the reservoir solution using a multi-channel pipette 7. Using a Mosquito robot (it uses fresh needles each time) Best, Vish From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Viswanathan Chandrasekaran Sent: Friday, March 08, 2013 4:24 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Off-topic: Fungal growth in robot trays Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don’t have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish
Re: [ccp4bb] Off-topic: Fungal growth in robot trays
We add 0.02% Azide to all the water used for washing in our Phoenix, and that seems to help. Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email janet.new...@csiro.au From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Viswanathan Chandrasekaran [v...@biochem.utah.edu] Sent: 09 March 2013 10:24 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Off-topic: Fungal growth in robot trays Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don’t have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish
Re: [ccp4bb] Off-topic: Fungal growth in robot trays
Viswan Try cleaning the supply carboy and lines with bleach. Flush thoroughly with DD water afterwards. Mark Sent from my iPhone On Mar 8, 2013, at 5:33 PM, Viswanathan Chandrasekaran v...@biochem.utah.edumailto:v...@biochem.utah.edu wrote: Dear All: I would like some advice on getting rid of persistent fungal growth in 96-well sitting drop crystal plates that were set up using a Phoenix robot. 24-well sitting drop trays prepared by hand don’t have this problem. Washing the robot with 0.5% bleach followed by plenty of water had no effect. Is adding sodium azide directly to commercial screen hotels (or the protein sample) a good idea? If so, how much should I add? Other suggestions are welcome. I will post a summary of all replies. Thank you. Best, Vish
