I wonder whether that is why it works as an anti-coagulant? Is it
mimicking nucleic acids? So then would DNA work as an anti-coagulant
as well...?
JPK
On Mon, Apr 11, 2011 at 4:20 PM, Nian Huang wrote:
> Heparin simulates the structure of DNA and RNA, so it has nonspecific
> affinity towards DNA
Heparin simulates the structure of DNA and RNA, so it has nonspecific
affinity towards DNA or RNA binding protein. It has also been used as DNAse
or RNase inhibitor but it is not very good one.
Nian Huang, Ph.D.
UT Southwestern Medical Center
On Sat, Apr 9, 2011 at 7:44 PM, Alexandra Deaconescu
w
(on behalf of Zhijie Li
>)
>Subject: [ccp4bb] immobilized DNA resin
>To: CCP4BB@JISCMAIL.AC.UK
>
>Hi,
>
>I have never done this myself, but as far as I know, DNA can be directly
>conjugated through their primary amino groups to CNBr-activated beads or
>NHS-activated
Hey, does Wikipedia lie? Also, the reference is to Lehninger's text,
although I did not verify it.
http://en.wikipedia.org/wiki/Heparin
On Sun, Apr 10, 2011 at 3:37 PM, Dima Klenchin
wrote:
>
>> heparin sulfate has the highest negative charge density of any known
>> biological molecule.
>
> Seem
heparin sulfate has the highest negative charge density of any known
biological molecule.
Seems to me that phytic acid (IP6, C6H6-(H2P04)6) and inositol
heptakisphosphate (IP7) should have higher charge per mass and per volume.
- Dima
Related fact I learned recently, perhaps of interest for fellow record-keepers:
heparin sulfate has the highest negative charge density of any known
biological molecule.
JPK
On Sat, Apr 9, 2011 at 9:38 PM, Raji Edayathumangalam wrote:
> Hi Alex,
>
> Most DNA-binding proteins has decent affinity
,
UK.
Tel: +44 (0) 207 594 5464
Fax: +44 (0) 207 594 3057
- Reply message -
From: "Raji Edayathumangalam"
To: "CCP4BB@JISCMAIL.AC.UK"
Subject: [ccp4bb] immobilized DNA resin
Date: Sun, Apr 10, 2011 03:38
Hi Alex,
Most DNA-binding proteins has decent affinity to h
Sent: Saturday, April 09, 2011 8:44 PM
To:
Subject: [ccp4bb] immobilized DNA resin
Hello ccp4 enthusiasts:
I am afraid this is a non-ccp4 related question. Can anyone recommend an
immobilized dsDNA chromatographic resin for purification of DNA-binding
proteins? GE seems to have something -
Hi Alex,
Most DNA-binding proteins has decent affinity to heparin columns. Have you
tried purifying your protein over heparin columns?
Cheers,
Raji
On Sat, Apr 9, 2011 at 8:44 PM, Alexandra Deaconescu
wrote:
> Hello ccp4 enthusiasts:
>
> I am afraid this is a non-ccp4 related question. Can a
Hello ccp4 enthusiasts:
I am afraid this is a non-ccp4 related question. Can anyone recommend an
immobilized dsDNA chromatographic resin for purification of DNA-binding
proteins? GE seems to have something - I was wondering if people have
other recommendations? In the age of GST and His tags
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