Re: [ccp4bb] Differentiating bound Mn Ca.

www.dbriggs.talktalk.net iChat AIM ID: DBassophile --- Anyone who is capable of getting themselves made President should on no account be allowed to do the job. - Douglas Adams -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre

Re: [ccp4bb] Stereo grpahics and Virtual Reality

(http://www.wiili.org/Wiimote) and have already been used for controlling industrial robots (http://www.youtube.com/watch?v=0qEotHQgUsg). Stephen -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN

Re: [ccp4bb] highly soluble proteins

crystallize it? I am considering trying salting out using dialysis, and also adding ligands/inhibitors. The protein is in 50 mM NaCl plus 50 mM buffer. I welcome thoughts and suggestions on crystallization ideas, publications, etc. Thank you Yvonne -- Dr Stephen Graham Nuffield

Re: [ccp4bb] extra density on Cysteine

Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] Vary b-factors from a plane/point.

ID: dbassophile -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549 from pymol import cmd from pymol import stored from math import

Re: [ccp4bb] anomalous signal of Mn and Ca ions

Never miss a thing. Make Yahoo your homepage. -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

[ccp4bb] multiple sequence alignment from multiple pairwise structural alignments

of pairwise sequence alignments) and calculate the 'best' multiple sequence alignment? Please note that there is absolutely no sequence conservation between these structures, making standard sequence-based alignment tools pretty useless. Thanks, Stephen -- Dr Stephen Graham Nuffield Medical

Re: [ccp4bb] discontinuous data wedges in XDS

in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: [EMAIL PROTECTED] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology

Re: [ccp4bb] problem of crystallization

clear still. Is there any suggestion for reducing protein solubility or increasing the chance of getting crystals? Thanks for your time, Jennifer -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN

Re: [ccp4bb] Google marks CCP4 web site as a potential security threat

by MailScanner, and is believed to be clean. -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] My cysteines

Stephen Graham Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] non-waters among structured solvent atoms

of my google searches has turned up the references I was looking for. Could someone point me in the right direction, please? Many thanks, Wolfram Tempel -- Dr Stephen Graham 1851 Research Fellow Cambridge Institute for Medical Research Wellcome Trust/MRC Building Addenbrooke's Hospital, Hills

Re: [ccp4bb] Off Topic: Isothermal Titration Calorimetry (ITC)

                     * *                                              * * E-mail: jbr...@itqb.unl.pt                   * * URL: http://mx.itqb.unl.pt                   * -- Dr Stephen Graham 1851 Research Fellow Cambridge Institute for Medical Research Wellcome Trust/MRC Building

Re: [ccp4bb] European IPTG suppliers

Santiago de Compostela Spain http://web.usc.es/~vanraaij/ -- Dr Stephen Graham Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

Re: [ccp4bb] off topic; alignment keeping upper and lower case

much appreciated.       regards        Martyn Martyn F. Symmons Department of Pathology University of Cambridge 'Gun fhaillin 'san fhairge..' (said of Griomasaigh boats) -- Dr Stephen Graham Division of Structural Biology Wellcome Trust Centre for Human

Re: [ccp4bb] Crystallization of lysine and arginine rich proteins

not succeed.  I think any compound that binds to the lysine/arginine side chains might affect the crystallization process thereby reducing the internal flexibility of protein.  Can anybody suggest some effective strategy for the crystallization? Thanks Umar -- Dr Stephen Graham 1851

Re: [ccp4bb] Anions in protein structures

on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. -- Dr Stephen Graham 1851 Research Fellow Cambridge Institute for Medical Research Wellcome Trust/MRC Building

Re: [ccp4bb] Per-residue RMSD for multiple structures?

for all four structures jointly.        Ethan -- Ethan A Merritt Biomolecular Structure Center University of Washington, Seattle 98195-7742 -- Dr Stephen Graham 1851 Research Fellow Cambridge Institute for Medical Research Wellcome Trust/MRC Building Addenbrooke's Hospital, Hills Road

[ccp4bb] Postdoc in Cambridge on Virus:Host interactions

cover letter that you’re applying for Position 3. Applications close on Tuesday, so there’s no time to waste! Cheers, Stephen Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP Ph: 01223

[ccp4bb] Postdoc in Cambridge on protein:protein interactions in eukaryotic membrane trafficking

/ and applications close on April 27th (only via the website please, it makes the HR people feel unloved otherwise). Cheers, Stephen Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP Ph: 01223

Re: [ccp4bb] Bond between Cys S and Met methyl group?

side chains, plus perhaps a close contact of the Met with Cys in one of the conformations. Sadly : ( Thanks all on and off-list for the good ideas! Stephen From: herman.schreu...@sanofi.com Sent: 17 July 2019 15:18 To: Stephen Graham; CCP4BB

[ccp4bb] Bond between Cys S and Met methyl group?

Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP http://www.path.cam.ac.uk/research/investigators/graham/ To unsubscribe from

[ccp4bb] Postdoc position in structural enzymology in Cambridge, UK

you might have. Cheers, Stephen -- Prof. Stephen Graham School of Biological Sciences Theme Leader: Infection and Immunity Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP http://www.atomicvirology.path.cam.ac.uk

Re: [ccp4bb] difficult MAD dataset

-spacegroups of P212121 - as Tassos will tell you this works a treat! Cheers, Stephen -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549

[ccp4bb] Risk assessment for heavy atom soaking - examples?

to share the risk assessments they have in their institute/company so that I can seek inspiration and make sure I'm keeping up with best practice. Many thanks, Stephen -- Dr Stephen Graham Sir Henry Dale Fellow and University Lecturer Department of Pathology University of Cambridge Tennis Court