Jun Yong and Jobichen
(I've mentioned this before, but ) - both of your projects jump out as very
good targets for microseeding with random screens. This method often gives
extra hits and better crystals because it is more likely that crystals will
grow in the metastable zone. It often reduces
Hey Jun,
If it's an old batch check and see if you have microorganisms living in the
stock or proliferating in the drop - see the paper by Bai et al:
doi:10.1107/S1744309107002904
In this paper they demonstrated how they could not reproduce a crystal hit
from an old screen up until they realized a
I once had a similar problem. The crystals only reproduced when grown in
condition containing PEG 8000 from SIGMA but not from FLUKA. This difference
may not affect crystals from other proteins but some proteins are more
sensitive to slight changes than others. Since you found out that the
Maybe your old solution evaporated then you end up in your old tube with a
more concentrated solution in PEG and NaCl so try to screen with new
conditions with higher PEG and/or NaCl concentration
Mick
2011/4/12 Jun Yong Ha j...@princeton.edu
Hi all,
Recently, I produced crystals with
or PEG 4000 got old. ask around in the department or university for old PEG
4000 bottles.
good luck!
Berta
On Apr 12, 2011, at 3:28 PM, mickael blaise wrote:
Maybe your old solution evaporated then you end up in your old tube with a
more concentrated solution in PEG and NaCl so try to
Hi,
Some anecdotes here for your reference:
One paper I read says that the authors were having trouble reproducing a
crystal from an initial screen. After some debugging, they realized that it
was because that they used a same pipette tip when making screens. Adding a
little solution from
PS, it might be a good time to start an additive screen.
--
From: Jun Yong Ha j...@princeton.edu
Sent: Tuesday, April 12, 2011 7:56 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Reproducing crystals.
Hi all,
Recently, I produced crystals with
Frances Jurnak published a paper in 1986 on PEG impurities and purification.
As I recall, it turns out that different manufacturers put different
additives in PEGs as preservatives. These are generally anti-oxidants.
PEGs do get oxidized.
I suggest you heat up your new PEG solutions to say 80
You might also try to control the degree of oxidation using the microwave, and
setting up trials after different numbers of cycles of heating.
Kendall
On Apr 12, 2011, at 12:41 PM, Jim Pflugrath wrote:
Frances Jurnak published a paper in 1986 on PEG impurities and purification.
As I
