ednesday, June 22, 2022 at 9:10 AM
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride
> >
> > I posted the following to ccp4bb in 2017 in response to a similar
> question about ADPBeF4. For AlF4 you’ll increase the NaF stoichiometri
lubility of NaF.
>
> From: CCP4 bulletin board on behalf of Dr. Kevin M
> Jude
> Date: Wednesday, June 22, 2022 at 9:10 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride
>
> I posted the following to ccp4bb in 2017 in response to a simi
:10 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride
I posted the following to ccp4bb in 2017 in response to a similar question
about ADPBeF4. For AlF4 you’ll increase the NaF stoichiometrically:
reagent[stock] (mM)final concentration (mM)volume (µl
is
quite acidic.
From: CCP4 bulletin board on behalf of Firdous Tarique
Date: Tuesday, June 21, 2022 at 5:13 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Solubility of Aluminum Fluoride
Thanks for your input. To avoid precipitation what about doing something like
this? For example, as we know
I have never tried varying the order of mixing, but it seems the correct
ADP-BeF3 complex was formed when mixing all three solutions just before use.
Guillaume
> On 22 Jun 2022, at 02:12, Firdous Tarique wrote:
>
> Thanks for your input. To avoid precipitation what about doing something like
Thanks for your input. To avoid precipitation what about doing something
like this? For example, as we know: AlCl3 + 3NaF = AlF3 + 3NaCl
Therefore, adding 100mM AlCl3 plus 300mM NaF to get 100mM of AlF3 solution,
then directly using this 100 mM stock of AlF3 for the final dilution, say 1
or 2 mM
Hi Firdous,
I have read a fair amount of papers about ATP-dependent chromatin remodelers in
which ADP-BeF3 is often cited. It is made as follows (quoting from one of these
papers): "ADP-BeF3 was prepared as a 10x stock (10 mM ADP, 30 mM BeSO4, 150 mM
NaF, 10 mM MgCl2), freshly before use."
So
Hi Firdous,
When I have used ADP/AF3 as a transition state analogue in crystallography, I
have always formed the ADP/AF3 complex in place by adding ADP, aluminum
nitrate, and fluoride at a 1:1:6 ratio to my protein. I then used that mixture
directly for crystallogenesis.
Best,
Michael
From:
