Re: [galaxy-dev] lastz on local galaxy failing

2012-11-26 Thread Andreas Kuntzagk
Hi, I just noticed that this was another case of a tool that needs more then the 1GB memory that is default on our cluster. After adjusting the job_runner settings everything seems fine. regards, Andreas On 15.11.2012 09:08, Andreas Kuntzagk wrote: Hi, when I start a lastz job it submits a

[galaxy-dev] multithreaded tools

2012-11-26 Thread Andreas Kuntzagk
Hi, I'm wandering how galaxy supports tools that are multithreaded or multi-process. When working with lastz I noticed that it starts 4 parallel processes. Is that always so? Can this be adjusted? What other tools also are multi-process? regards, Andreas -- Andreas Kuntzagk

Re: [galaxy-dev] multithreaded tools

2012-11-26 Thread Bossers, Alex
Andreas, Yes this is possible. You can also have a look at the ncbi blast+ tools written by Peter. The same is true. Usually the tool wrappers (xml) have an option preconfigured how many threads can be used... You can adjust these directly in the xml OR as we did we added the option to be

[galaxy-dev] how to get annotation files for rna-seq

2012-11-26 Thread Dr. Mira A. Bisso
Dear help; How to get the human annotation file for RNAseq analysis, for mapping by BWA and for RNAseq analysis, on Cufflinks and cuffdiff tools on galaxy? Mira Thanks [image001] Mira Bosso, M.Sc, B.D.S Research associate Pancreatic Islet Biology and Transplantation Unit P.O.Box 1180,

Re: [galaxy-dev] how to get annotation files for rna-seq

2012-11-26 Thread Joachim Jacob
You can use 'Get data' -- 'UCSC Main' to fetch an GTF (gene transfer format) format of your species of interest, Human in this case. I tend to use the Ensembl genes track (from the Genes and gene prediction tracks). Make sure you use GTF as output, and send to Galaxy! Cheers, Joachim Joachim

Re: [galaxy-dev] XGRID and Galaxy

2012-11-26 Thread Glen Beane
XGrid is a dead-end technology. It has been removed from OS X starting with Mountain Lion (10.8). -- Glen L. Beane Senior Software Engineer The Jackson Laboratory (207) 288-6153 On 11/22/12 9:47 PM, James Boocock smilefreak2...@gmail.com wrote: Hi Galaxy Dev Team. We are looking to put

[galaxy-dev] help

2012-11-26 Thread Magriet Van Der Nest
Dear Galaxy help-centre I want to use Tophat and Clifflinks to do an expression analysis. However, the reference genome for Heterobasidion annosum (it is available at JGI) is not listed. You have posted on your website that we can contact you if 'your genome of interest' is not listed. Is it

[galaxy-dev] Re. RNA-Seq

2012-11-26 Thread Songyan Liu
I was using Cufflinks to analyze Clip-Seq data. The results as 'gene expression' and 'transcript expression' gave me almost same results (No. of genes ? No. of transcripts). I guess the number of genes should be relatively smaller than that in transcripts. I wonder if I need to get a special

[galaxy-dev] FW: Re. RNA-Seq

2012-11-26 Thread Songyan Liu
My account emailis so...@cc.umanitoba.ca From: Songyan Liu [mailto:m...@cc.umanitoba.ca] Sent: Friday, November 23, 2012 1:41 PM To: 'galaxy-dev@lists.bx.psu.edu' Subject: Re. RNA-Seq I was using Cufflinks to analyze Clip-Seq data. The results as 'gene expression' and 'transcript

Re: [galaxy-dev] how to get annotation files for rna-seq

2012-11-26 Thread shamsher jagat
Can we also fetch virus file from some sources like this? Thanks Kanwar On Mon, Nov 26, 2012 at 4:25 AM, Joachim Jacob joachim.ja...@vib.be wrote: You can use 'Get data' -- 'UCSC Main' to fetch an GTF (gene transfer format) format of your species of interest, Human in this case. I tend to

Re: [galaxy-dev] Can't edit galaxy workflow

2012-11-26 Thread Liisa Koski
Hi, Can you please send the entire link to access this changeset? I keep searching for 5013377e0bf7 but can not find it. Thanks in advance, Liisa From: Dannon Baker dannonba...@me.com To: Sanjarbek Hudaiberdiev hudai...@icgeb.org Cc: galaxy-...@bx.psu.edu Date: 13/11/2012 12:34

Re: [galaxy-dev] lastz on local galaxy failing

2012-11-26 Thread Bob Harris
Howdy, Andreas, Lastz memory requirements are dependent on the size of the input sequences (mainly on the size of reference sequence) and, to a lesser extent, the genome's repeat content. I'm a little confused/concerned by how this failure was indicated. When run from a console, if

Re: [galaxy-dev] multithreaded tools

2012-11-26 Thread Bob Harris
Howdy, Andreas, The four processes started for a galaxy lastz job must involve post- processing the lastz output through some other shell tool. Lastz by itself doesn't support multiple threads or processes. Bob H On Nov 26, 2012, at 3:58 AM, Andreas Kuntzagk wrote: Hi, I'm wandering

Re: [galaxy-dev] Can't edit galaxy workflow

2012-11-26 Thread Scott McManus
Bitbucket will only show the first 7 characters when browsing through commits. Try this: https://bitbucket.org/galaxy/galaxy-central/changeset/5013377e0bf7a656ea593098f1d1b38f3d6928c6 -Scott - Original Message - Hi, Can you please send the entire link to access this changeset? I

Re: [galaxy-dev] Galaxy IGV display error

2012-11-26 Thread Daniel Blankenberg
Hi Joan, For IGV and most external displays that work on BAM and other larger seekable file formats (bigwig, bigbed, etc.) you'll need to use and configure a proxy server for your local Galaxy instance that provides byte-range request capabilities, such as nginx

Re: [galaxy-dev] Folder permissions after cluster run

2012-11-26 Thread Nate Coraor
On Nov 19, 2012, at 10:26 AM, Sajoscha Sauer wrote: Hi, We are running galaxy on the cluster with the 'real user' submission. It all works fine, but at the end of the run we found something unexpected. All the .dat files in the file_path folder are owned by the user running Galaxy,

Re: [galaxy-dev] Problem with Active Directory/LDAP authentication

2012-11-26 Thread Nate Coraor
On Nov 19, 2012, at 12:49 PM, Harris Shapiro wrote: Hello, I'm writing because I've been trying for the past few days to configure Galaxy to use Apache-based LDAP authentication, but have reached a point where I'm basically stuck. The system in a virtual machine running: - CentOS 5.8

Re: [galaxy-dev] how to get annotation files for rna-seq

2012-11-26 Thread Jeremy Goecks
Thanks Jeremy, Are there any resources linked with Galaxy for viral genome seq annotation ? I'm not aware of any viral genome annotations resources integrated into Galaxy, but NCBI (and others) have virus gene annotations that you upload into Galaxy and use. Best, J.

[galaxy-dev] No output from IGVtools tile

2012-11-26 Thread Sytchev, Ilya
Hi, I'm running IGVtools (http://toolshed.g2.bx.psu.edu/repos/jjohnson/igvtools) tile tool in Galaxy on a WIG file but it's failing with exit code 1 and producing no output file. On the other hand, IGVtools count and sort run successfully in Galaxy on their respective inputs. Also, IGVtools