Re: [galaxy-user] Accessing Galaxy on the cloud from http

2011-09-27 Thread Colleen Doherty
Thanks for the help! I was able to successfully connect and get galaxy running. The items I had incorrect and needed to change were: One security group User data with NO <> and only a space between the : and the data (Not a tab). Thanks f the quick responses and advice, I would have been forever t

Re: [galaxy-user] Accessing Galaxy on the cloud from http

2011-09-27 Thread Brad Chapman
Colleen; Yoy definitely only want to select a single security group: the CloudMan one. Selecting multiple groups like uses the most conservative settings in the union of the groups, so won't have the right ports open for CloudMan. > The ami I've been mainly using is ami-da58aab3 (although I've tr

Re: [galaxy-user] How to analyze Genes Differential Expression using Galaxy? Thanks.

2011-09-27 Thread Jennifer Jackson
Hello Bin, If your data is RNA-seq, then this tutorial can get you started with the basics: http://main.g2.bx.psu.edu/u/jeremy/p/galaxy-rna-seq-analysis-exercise http://main.g2.bx.psu.edu/u/jeremy/p/transcriptome-analysis-faq Hopefully this helps, Best, Jen Galaxy team On 9/26/11 8:46 AM, B

Re: [galaxy-user] Question

2011-09-27 Thread Jennifer Jackson
Hello Diana, For the main public Galaxy server, an input sequence file of this size should not be a problem when analyzed through the standard tools. Hard quotas are not set at this time, but will be announced soon. Current guidelines can be found at: http://galaxyproject.org/wiki/Main Hope

Re: [galaxy-user] run tophat in galaxy

2011-09-27 Thread Jennifer Jackson
Hi Tao, Yes, the resulting SAM dataset can be converted to BAM and viewed in the GTB (Galaxy Track Browser). http://galaxyproject.org/wiki/Learn -> scroll to "Visualization" to find: http://galaxyproject.org/wiki/Learn/Visualization The GTB can be reached through a different links, but one q

Re: [galaxy-user] mm9 reference GTF file for Cuffcompare (shamsher jagat)

2011-09-27 Thread Lozach, Jean
-- Message: 4 >Date: Mon, 26 Sep 2011 16:13:54 -0700 >From: shamsher jagat >To: galaxy-user >Subject: [galaxy-user] mm9 reference GTF file for Cuffcompare >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >I wonder if some has mouse genome GTF fi

Re: [galaxy-user] Injecting External File References

2011-09-27 Thread Nate Coraor
Oren Livne wrote: > Dear All, > > We have a lot of sequencing data files whose locations and > identifiers are managed by our home-grown webapp. We'd like to > enable users to single-sign-on from our webapp into Galaxy (we will > be using open ID on both systems for that). When the user opens a >

[galaxy-user] Problem in merging two BAM file in Galaxy. Thanks for help.

2011-09-27 Thread Binbin You
Hi All, I tried many times to merge two BAM files (3.3 GB and 7.7MB) using different versions of Galaxy. But every time I only  got an empty file and it showed some info like this: [Wed Sep 14 08:56:06 EDT 2011] net.sf.picard.sam.MergeSamFiles done. Elapsed time: 0.02 minutes. Runtime.totalMem

[galaxy-user] Accessing Galaxy on the cloud from http

2011-09-27 Thread Colleen Doherty
Thanks for the suggestions! The ami I've been mainly using is ami-da58aab3 (although I've tried a few others). The user data may be where I am messing up:  I have a few questions I'm not clear on, I think I've tried may options here.. 1.  Is cluster name just something I make up? 2.  Are the <> c