[galaxy-user] When will megablast be working again

2014-04-28 Thread Scott W. Tighe
Dear NCBI and Galaxy Team I have sent a note to determine when the Megablast option will be active again. Whether you use Galaxy or NCBI directly, it is inoperative. Scott -- Core Laboratory Research Staff Advanced Genome Technologies Core Deep Sequencing (MPS) Facility Vermont Cancer Cen

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Scott W. Tighe
Dear Wayne Here is the error message I get back. Server Error Your request could not be processed due to a problem on our Web server. This could be a transient problem, please try the query again. If it doesn't clear up within a reasonable period of time, e-mail a short description of you

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Peter Cock
Hi Scott, Could you clarify *which* Galaxy server you are using, and *which* megablast tool within Galaxy? Most BLAST options within Galaxy do NOT send the queries to the NCBI servers. (1) Main Public Galaxy I would guess you are using the main Galaxy Server, which only has this megablast wrappe

Re: [galaxy-user] Megablast database identity

2014-04-28 Thread Jennifer Jackson
Hello, The Megablast htgs, nt, and wgs databases are in the process of being updated to the latest NCBI releases and are expected to be available by tomorrow morning (possibly sooner). Should you wish to continue your analysis using the prior versions, these are available through our rsync s

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Jennifer Jackson
Hi Peter, Thanks for clarifying all of this! Main (usegalaxy.org) was restarted, perhaps why you encountered an intermittent access delay. Let us know if are still unable to reach. For the megablast issue over the weekend, we are updating the databases. I replied to Scott's first galaxy-u...

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Scott Tighe
Peter Thank you for your detailed response and I should have noted that I was using the Main Public Galaxy. Thank you for confirming that it is down! I appreciate your input! Scott Scott Tighe Senior Core Laboratory Research Staff Advanced Genome Technologies Core NextGen Sequencing/Flow Cyt

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Peter Cock
On Mon, Apr 28, 2014 at 9:01 PM, Scott Tighe wrote: > Peter > > Thank you for your detailed response and I should have noted that I was > using the Main Public Galaxy. Thank you for confirming that it is down! > > I appreciate your input! > > Scott Hi Scott, Galaxy is back for me now, and yes, t

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Scott Tighe
Peter Actually I was running blast separately and got error messages from them as well and I assumed it was a connection problem But in fact it was two separate problems unrelated. Good catch And thanks again Scott Vermont Cancer Center Advanced Genome Techology Lab On April 28, 2014 4:28:49

Re: [galaxy-user] [blast-help] When will megablast be working again

2014-04-28 Thread Matten, Wayne (NIH/NLM) [C]
Hello, Scott, The web blast service, including megablast, is functioning normally. I don't know how Galaxy submits searches to us, but my guess is via our blast URLAPI, which is also functioning well at the moment. If your searches using our web page directly are also not working, please send me