[galaxy-user] Trackster Error: needLargeMem: trying to allocate 0 bytes (limit: 100000000000)

2013-10-30 Thread Guest, Simon
I'm having problems getting Trackster working on my own Galaxy
instance, so I thought I would check on the usegalaxy public server.

However, I'm getting the same Trackster Error: needLargeMem: trying to
allocate 0 bytes (limit: 1000) that was reported on this list
in July, but there was no followup:
http://user.list.galaxyproject.org/Trackster-Error-td4655737.html

My history is at https://usegalaxy.org/u/simon-guest/h/trackster-error

This is just an artificial test I made using a fragment of a reference
genome, but I thought it should work OK.

Any clues?

cheers,
Simon
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Re: [galaxy-user] Trackster Error: needLargeMem: trying to allocate 0 bytes (limit: 100000000000)

2013-10-30 Thread Björn Grüning
Hi Simon,

I had that error before, not with trackster ... somewhere else. I think
it's an error of one of these UCSC tools and it occurs if you supply
empty files. Can you check that none of your file is empty? Or your file
will be automatically converted during trackster visualisation and that
is resulting in an empty file. 

Just a guess ...
Cheers,
Bjoern


 I'm having problems getting Trackster working on my own Galaxy
 instance, so I thought I would check on the usegalaxy public server.
 
 However, I'm getting the same Trackster Error: needLargeMem: trying to
 allocate 0 bytes (limit: 1000) that was reported on this list
 in July, but there was no followup:
 http://user.list.galaxyproject.org/Trackster-Error-td4655737.html
 
 My history is at https://usegalaxy.org/u/simon-guest/h/trackster-error
 
 This is just an artificial test I made using a fragment of a reference
 genome, but I thought it should work OK.
 
 Any clues?
 
 cheers,
 Simon
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
   http://lists.bx.psu.edu/listinfo/galaxy-dev
 
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 please use the interface at:
 
   http://lists.bx.psu.edu/
 
 To search Galaxy mailing lists use the unified search at:
 
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Re: [galaxy-user] Trackster Error: needLargeMem: trying to allocate 0 bytes (limit: 100000000000)

2013-10-30 Thread Jeremy Goecks
It turns out that your artificial test is a bit too artificial. In order to 
display a coverage plot, Trackster converts reads in a BAM to BigWig using a 
two step process:

(1) BAM to bedgraph;
(2) bedgraph to bigwig

Your super simple example generates an empty file in step 1 because your single 
read does not map to Araly1, and the tool used in step 2 (bedGraphToBigWig) 
fails with the error that you're seeing. This is a corner-case bug, and I've 
create a card for this so you can track its resolution: 
https://trello.com/c/kMFUNawL

Best,
J.


On Oct 30, 2013, at 5:44 PM, Guest, Simon simon.gu...@agresearch.co.nz 
wrote:

 I'm having problems getting Trackster working on my own Galaxy
 instance, so I thought I would check on the usegalaxy public server.
 
 However, I'm getting the same Trackster Error: needLargeMem: trying to
 allocate 0 bytes (limit: 1000) that was reported on this list
 in July, but there was no followup:
 http://user.list.galaxyproject.org/Trackster-Error-td4655737.html
 
 My history is at https://usegalaxy.org/u/simon-guest/h/trackster-error
 
 This is just an artificial test I made using a fragment of a reference
 genome, but I thought it should work OK.
 
 Any clues?
 
 cheers,
 Simon
 ___
 The Galaxy User list should be used for the discussion of
 Galaxy analysis and other features on the public server
 at usegalaxy.org.  Please keep all replies on the list by
 using reply all in your mail client.  For discussion of
 local Galaxy instances and the Galaxy source code, please
 use the Galaxy Development list:
 
  http://lists.bx.psu.edu/listinfo/galaxy-dev
 
 To manage your subscriptions to this and other Galaxy lists,
 please use the interface at:
 
  http://lists.bx.psu.edu/
 
 To search Galaxy mailing lists use the unified search at:
 
  http://galaxyproject.org/search/mailinglists/


___
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Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
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Re: [galaxy-user] Trackster Error: needLargeMem: trying to allocate 0 bytes (limit: 100000000000)

2013-10-30 Thread Guest, Simon
At Wed, 30 Oct 2013 21:51:05 -0400,
Jeremy Goecks wrote:
 
 It turns out that your artificial test is a bit too artificial. In order to 
 display a coverage plot, Trackster converts reads in a BAM to BigWig using a 
 two step process:
 
 (1) BAM to bedgraph;
 (2) bedgraph to bigwig
 
 Your super simple example generates an empty file in step 1 because your 
 single read does not map to Araly1, and the tool used in step 2 
 (bedGraphToBigWig) fails with the error that you're seeing. This is a 
 corner-case bug, and I've create a card for this so you can track its 
 resolution: https://trello.com/c/kMFUNawL

Hi Jeremy, Bjoern,

Thanks for your help.  Yes, my data file wasn't very good.  I tried again with 
a more realistic sample, and it worked fine.

Sorry for the noise.

cheers,
Simon
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Galaxy analysis and other features on the public server
at usegalaxy.org.  Please keep all replies on the list by
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