Re: [Histonet] Average number of slides reviewed by a surgical pathologist

Please go to: http://www.histosearch.com/rene.htmland download my article on Staffing. The answer to your question is there.René On Monday, August 3, 2015 5:20 PM, Vickroy, James via Histonet histonet@lists.utsouthwestern.edu wrote: I realize this is a question that may be

Re: [Histonet] Handline paraffin

It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or irritating substance. It is just an oil of high molecular weight (mineral oil)René On Monday, August 3, 2015 12:48 PM, Johnson, Carole via Histonet histonet@lists.utsouthwestern.edu wrote:

Re: [Histonet] Handline paraffin

AM, Rene J Buesa via Histonet histonet@lists.utsouthwestern.edu wrote: It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or irritating substance. It is just an oil of high molecular weight (mineral oil)René     On Monday, August 3, 2015 12:48

Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions

1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount.2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I

Re: [Histonet] Xylene Free Labs - One Final Question

I always bought my mineral oil from Jim Coyle Associates but the brand was PENRECO and I am sure you can ask them any supplier near your lab.This is the cheapest way of buying mineral oil, otherwise (from a pharmaceutical company) it will be EXTRAORDINARILY expensive.René On Friday,

Re: [Histonet] Film Coverslip

If you are using the Sakura instrument, please do not use other film than Sakura's. It is not only much better but also will allow the coverslipper to work better.Sometimes a cheaper option will be more costly at the end.René On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet

Re: [Histonet] understanding reagents in decalcifier; making it in-house

If you can make the decalcifier with that recipe, you will be OK. You can even calculate costs and probably it will be cheaper than buying it from Shandon.René On Wednesday, July 22, 2015 4:36 PM, M.O. via Histonet histonet@lists.utsouthwestern.edu wrote: Hello Histonet, The

Re: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens

As I see it, the only way this system may work is if you have a code to determine what those random numbers mean and which samples they belong to which, in itself, will defeat the randomization objective.Otherwise this will be chaos in any lab, and the bigger the greater the chaos.To me it is

Re: [Histonet] Running a histo lab without a histotech?

About the "legality" I do not think it is of your direct concern, unless you want to "challenge" the situation with some sort of legal action, which is really not advisable.As a functioning laboratory in a hospital it has to be under the supervision of either CLIA or  ASAP and the pathologist

Re: [Histonet] Xylene and Formalin substitutes

The most straight forward solution to your problem is the following: 1- change from ethanol dehydration to 2-propanol (isopropyl-alcohol)2- after 100% propanol, go directly a mixture 1:1 of propanol with mineral oil light molecular weight3- go to your regular paraffin embedding steps. These

Re: [Histonet] plant tissue stain

I always used a green (Fast green) for cell walls and cytoplasm + a red (fuchsine) for nuclei.If you get Peter Gray's book you will find numerous plant procedures. In Bolles-Lee (Microtomist's Vade-Mecum) there are also many methods for plant tissues.René On Saturday, November 7, 2015

Re: [Histonet] Competency Assessments

Elaine:As you wrote there are differences of opinion, so here is mine:Start with "analysis" which is the process of determining the qualities of something. As I see it, in histology the pathologist is the one who analyzes = determines the qualities of the tissue sections and gets to a

Re: [Histonet] IHC on old slides

The only thing you can do is to prolong the Ab staining time but, in order to avoid excessive background, dilute it. You will have to find an adequate balance between a more diluted Ab with a weaker spitope signal and a  prolonged incubation time and there is no "magic formula" for obtaining

Re: [Histonet] Histology Related Questions?

That is OK with me.René On Thursday, November 12, 2015 7:48 PM, Va Paula Sicurello via Histonet wrote: Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions.  

Re: [Histonet] Histonet Digest, Vol 144, Issue 14

That is a question completely dependent on your pathologist. S/he is the one taking those decisions.René On Monday, November 16, 2015 11:04 AM, Nexgen Pathology via Histonet wrote: Hi, im a budding histotechnician at Nexgen Pathology Limited in

Re: [Histonet] Microtomy statisitcs

24René J. On Monday, October 12, 2015 1:29 PM, "ODea, Elise via Histonet" wrote: I imagine there are several schools of thought on this topic, but here goes:  How many blocks should a histotech cut for 1 H per hour?  Other duties during this

Re: [Histonet] Microtome suggestions

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) manufactures the best, but you could try an OMS from Reichert although I am not sure they stll are manufactured because Leica swallowed Reichert some years ago.Another alternative, and probably even better than the

Re: [Histonet] Recut Rates?

Please go to: http://www.histosearch.com/rene/html to find answer to your query. René On Tuesday, August 25, 2015 6:48 PM, Pele Conqueror via Histonet histonet@lists.utsouthwestern.edu wrote: Good Afternoon Netters, What do you all consider a good recut rate? I'm going for an

Re: [Histonet] Anonymizing numbering system

Good! So probably when you really mess-up somebody's diagnose and have to pay several million of dollars in a law suit, your higher echelons will realize how stupid is the idea.Besides if you give a randomized number to a specimen and later use a code to decipher the randomized number, where is

Re: [Histonet] Blog Post--not work related

Why do you keep posting "not work related" things?I do not think this is proper, even if you think they are.It is annoying having to "spam" your postings.René On Thursday, December 17, 2015 1:23 PM, Lester Raff MD via Histonet wrote: We are making

Re: [Histonet] Collodian bag for cell blocks

Do you mean "collodion"? If so it is nitrocellulose, HIGHLY flammable and even explosive. Has a characteristic "pungent" odor.Highly unsafe and it is beyond me why would you use this product. The odor can "adhere" to clothing.You may have a ventilation system that "passes inspection" but if

Re: [Histonet] Not work related, but one of the last

Thank God!Not a second too early! René On Tuesday, December 29, 2015 1:25 PM, Linda via Histonet wrote: This is a professional histology information exchange.        From: Lester Raff MD via Histonet To:

Re: [Histonet] Off topic posts

So do I! René On Monday, December 21, 2015 12:28 AM, Anne Van Binsbergen via Histonet wrote: Dear Lester Raff MD Yes some of us do visit the Histonet over weekends. Your posts are so annoying and self-serving. Please go away! Thanks Annieinarabia

Re: [Histonet] HT/HTL vs MT

It depends if the registered MT has taken histotechnology theory and practice. If the MT has not taken those studies, the MT cannot substitute a registered HT/HTL.On the other hand, a registered HT/HTL for sure cannot do what a registered MT does, just because of the same reason, lack of theory

Re: [Histonet] Off topic posts

mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Dec 21, 2015, at 6:18 AM, Rene J Buesa via Histonet > <histonet@lists.utsouthwestern.edu> wrote: > > So do I! > René > >    On Monday, December 21, 2015 12:28 AM, Anne Van Bi

Re: [Histonet] DIF on paraffin embedded tissue

No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: We have a tissue sample that was processed and paraffin embedded.  We URGENTLY

Re: [Histonet] Are gloves required when cutting FFPE blocks?

NOT AT ALL, unless you are sectioning a suspected or known Creutzfeldt-Jakob's disease case. Check CAP regulations.René On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" wrote: Can anyone provide a reference as to whether or not

Re: [Histonet] Rapid tissue Programs

You point out to several issues that I would like to address:1- "nuclear bubbling" has nothing to do with processing. This is a post-sectioning artifact appearing when there is water underneath the section when it is set to dry before staining. Just make sure you shake the slide with the

Re: [Histonet] Duraedge (Black) help

Extremely rare this problem because almost always thick/thin sections has nothing to do with the blades but with the microtome that is unable to hold the blade firmly.Are the blades in this microtome "thinner" than the others used in it previously?If they are, you could add a small piece of

Re: [Histonet] retaining cut slides to keep or not to keep

It seems you have "baked" and "unbaked" slides.4ºC storage is always more expensive and "baked" slides are keep very well at RT.I think your first step is to ask around who would like those specific slides.If they will be used in the future, "bake" those "unbaked" and store all at RTRené

Re: [Histonet] Spill kits

What you need to do is to communicate to everybody where the kits are, and place them where it is more convenient for you. Once everybody knows the location, a good sign is always a plus.René On Tuesday, June 14, 2016 10:15 AM, Anne Murvosh via Histonet

Re: [Histonet] Over-processing of brain tissue

It seems to me you are processing too much unless the slices are 3mm thick or more.I suggest you to cut the dehydration to 45 minutes (the sequence seems OK)Reduce the pure 2-propanol to just 2 changes (30 min is OK)Add 1 change of a mixture 1:1 of 2-propanol and xylene + 2 xylene stepsthen to

Re: [Histonet] Powder Picric Acid vs Liquid Picric Acid

If your 1.3% picric solution is in distilled water, there is not much you can do about it.If it is in acetone 38.4 mL contains 0.5 g of picric acid to which you can add 361.6 mL of acetone to get your desired concentration.René On Monday, June 13, 2016 3:24 PM, Jennifer MacDonald via

Re: [Histonet] Histology tips

Would you share what you receive for the amusement/benefit of us all?René On Tuesday, May 31, 2016 9:15 AM, Charles Riley via Histonet wrote: I am trying to do a histology tip of the week for my new histo team as a way to help them learn some new ways

Re: [Histonet] consult requests

We used to charge the person (patient, hospital or pathologist) who requested the consult.René On Thursday, January 14, 2016 1:01 PM, Noelle Linke via Histonet wrote: Hi all, Question for anyone who may handle admin staff:  If a patient or an

Re: [Histonet] Credentials

Yes, BUT, in states where a certification/license is required to work in Histology (such as Florida) state requirements supersede CAP's and the inspectors have to oblige.René  On Friday, February 12, 2016 3:19 PM, Terri Braud via Histonet wrote:

Re: [Histonet] ORO staining in pre- vs. post-fixed tissue

Michele:Methodologically it is much more difficult to freeze/section formalin fixed tissue so it is unnecessary adding an additional layer of difficulty to the procedure pre-fixing in formalin.Although I have not tried it, there is no effect of NBF on the amount of fat so there should not be a

Re: [Histonet] Nuclear bubbling

"Nuclear bubbling", manifested as round unstained areas in the nucleus, is caused by incomplete dehydration of the section before staining. There is a review on the subject that I cannot find at this moment.René On Friday, January 29, 2016 10:42 AM, "Vickroy, James via Histonet"

Re: [Histonet] Clearance Angle on Microtome Blade

The type of tissue, the speed of sectioning, the knife bevel and the type of paraffin (melting point) influence the clearance angle.Anywhere from 5 to 10º (preferable 5-6º) are the most used.René On Friday, January 29, 2016 3:17 PM, Kelli Goodkowsky via Histonet

Re: [Histonet] Tissue processing question

Sponges can cause a compression artifact leaving some sort of "imprint" on the surface of the biopsy, especially kidney and prostate Bx.I my experience tissue paper is the best option. If you are having difficulties with the wrapping, you can use "tea bags".René  On Friday, January 29,

Re: [Histonet] Thanks...also blog post

Long live the subterfuge! René On Thursday, January 21, 2016 8:04 PM, Caroline Miller via Histonet wrote: Thank you Lester, this is a great middle ground! As someone who was a little put out by your totally off-topic emails, I am totally OK with you

Re: [Histonet] Nuclear Bubbling

If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the

Re: [Histonet] Nuclear Bubbling

Willem: Essentially incomplete deparaffination shows a different pattern and is not limited to nuclei only.Again, this is what the consensus is:1- there is ALWAYS  water left underneath the section2- that water HAS to be eliminated before the section is dried in the oven3- the best sectioning

Re: [Histonet] question - Allergy to histological solvents?

You can dewax absolutely safely using a 2% dishawasher soap solution at 90ºC (twice) as washing in water.You can "dehydrate" stained stains by placing the slides in an oven at 60ºC, also absolutely safely for the stained section.Under separate cover I am sending  articles on this subject.René

Re: [Histonet] H automated stainers

Before deciding, ask for a "demo" from Sakura.René On Monday, April 11, 2016 11:02 AM, Lauren Marie Hegner via Histonet wrote: Hello all, Our lab is looking for a new automated H stainer and I was wondering if anyone out there has had any

Re: [Histonet] Porcessing FFPE tissue without alcohol??

To embed the tissues with paraffin you HAVE TO dehydrate the tissue. This is usually done with either ethanol of 2-propanol but essentially all dehydrants will remove fat so you are right, the way to go is going frozen sections.René On Thursday, March 24, 2016 2:24 PM, "Dessasau III, Evan

Re: [Histonet] Porcessing FFPE tissue without alcohol??

The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.René On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote:

Re: [Histonet] Porcessing FFPE tissue without alcohol??

Sudan Black reacts only with protein-combined fats.René On Saturday, March 26, 2016 11:20 AM, Joanna <jobalu...@gmail.com> wrote: How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet > <histonet@lists.utsouthwestern.edu> wrote: >

Re: [Histonet] Whale skin

How did you manage to deal with the about 0.5 m of blubber?Was it the skin of a new born whale? I just to not understand, but you have to completely eliminate all the fat and increase your processing protocol (infiltration specially) to have some chance of getting any relatively "decent"

Re: [Histonet] Medical/health related post

Bryan:1- who gave permission to Dr. Raff?2- how we know the permission was given?3- what percentage of HistoNet member gave the permission?4- why Dr. Raff is so stubborn to keep posting what ever he chooses in spite of the rejection of probably more members than those who "gave him

Re: [Histonet] Using same DAB for multiple slide racks

This is not a good practice and can lead to inconsistent results.Always use freshly prepared DAB sol. to finally be able to see the end product of the costly and important IHC procedure, it is worth it.René On Monday, April 25, 2016 6:27 PM, Andrea Calhoun via Histonet

Re: [Histonet] vibrations

Photomicrography could be affected at high resolution (immersion oil objectives) but probably could be eliminated if the microscope table is isolated from the floor with some vibration damping device.René On Tuesday, May 17, 2016 9:52 AM, Terri Braud via Histonet

Re: [Histonet] PAS/Decal Question

My impression is that your problem is during the decalcification step. It cannot be hurried and has to be in EDTA at pH 7All reagents have to be prepared in pH7 phosphate buffer.The inconsistency resides in the fact that not all core Bx are the same regarding thickness, tissue condition or

Re: [Histonet] PAS Stain

As I see it, there are 3 main objections about using human saliva as an amylase source.In order of importance they are:1- you will never know the actual concentration of the amylase and this will produce reproducibility problems.2- along with the saliva you will introduce bacteria that may end

Re: [Histonet] picric acid

Picric acid is an expensive reagent useful in many histology procedures.The advise you received of adding water is a good one.Humid picric acid will not explode at all. Why waste a good reagent?Keep humid, you will eventually used it.René On Thursday, May 5, 2016 3:24 PM, Mca Werdler via

Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you VERY MUCH!René On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post

Re: [Histonet] Automated IHC instrument

I tested those you mention and leased/used the one from DAKO and "never looked back".René On Wednesday, May 4, 2016 12:13 PM, "Murphy, Valerie via Histonet" wrote: Hello Histonetters, Our tissue core is interested in purchasing an IHC instrument. It

Re: [Histonet] replacing a stainer and a coverslipper question

Productivity and quality sake, Sakura film coverslipper has no match. If you use Sakura tape and the xylene dispenser is properly calibrated, storage is not an issue.Sakura stainer was also what I used at my lab and I highly recommend both.René On Wednesday, May 4, 2016 3:19 PM, Jenn via

Re: [Histonet] Cytology/Histology Staining Question

I would be concerned with potential cross-contamination. In my lab we had 2 staining instruments, one for cytology and other for histology.René On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" wrote: Hello all, I work in a small, low

Re: [Histonet] Melanin Bleach

You are right. Bleaching is a "rough" procedure for the "survival" of sections and if on top of that you left the section overnight in DiH2O that is a recipe for disaster, as the one you experienced. Try to do the whole procedure during the same day.Additionally it seems to me that 6h in

Re: [Histonet] Fwd:

As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,→cut 1 section and stain → select at least 2 (+) areas→ divide the block into 2 blocks each containing one of those 2 areas and by doing so you would have duplicated the number of possible (+) sections.René

Re: [Histonet] copper stain

Timm's silver stain always!René On Wednesday, April 20, 2016 12:51 PM, Gudrun Lang via Histonet wrote: Hi all! Which stain would you prefer to demonstrate copper? Rhodanin or Victoria blue? Thanks in advance Gudrun

Re: [Histonet] Blog Post Not lab related

Amen!!!But you have to concede that Lester is a very  persistent, almost obstinate individual, probably used to impose his will and this postings are  just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know

Re: [Histonet] Pap stain without xylene

Hi Mike:The steps you desrcibe are wrong.After you finish staining your PAP smear, just wash them in the last ethanol → oven dry at 60ºC for 5 minutes or as required if the smear is too thick and when completely dried → coverslip.This final drying has to take place at temperatures above room

Re: [Histonet] Cleaning Tissue Molds

Place your molds in a 2% dishwasher soap boiling solution for 5 minutes → was in running water for 5 minutes → dry in a convection oven at 60ºC for 10 minutes and your molds will be ready to use.As a "release" solution use a mixture 1:1 of 2-propanol and mineral oil (light weight).René On

Re: [Histonet] block scrapers

Use a regular one blade pocket knife (as I used to do).René On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet wrote: Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone

Re: [Histonet] Feedback on Immunohistochemistry stainers

Request a DAKO demo.René On Thursday, July 28, 2016 8:01 PM, Gmail via Histonet wrote: Hi all, We are looking into getting a new staining platform for our IHC lab. I would appreciate any feedback from your experience regarding ease of use, how long

Re: [Histonet] On the hunt for new microtomes!

OCASTRA laboratories BUT I have never heard that it has bought Sakura instruments. It would be nice if somebody has reliable information about this alleged acquisition.René  On Monday, August 1, 2016 4:02 PM, Rene J Buesa via Histonet <histonet@lists.utsouthwestern.edu> wrote:

Re: [Histonet] On the hunt for new microtomes!

I don't know now, but some years ago Thermo instruments were less that reliable. Try Leica or even better Sakura.René On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet wrote: Hey HistoNet, Thanks to everyone who helped me out by

Re: [Histonet] specimen storage cabinets

Get regular metal cabinets used to store garage items.They are sold at any general store (such as HomeDepot or Walmart).René On Thursday, August 11, 2016 12:05 PM, Atoska Gentry via Histonet wrote: Hello, I work for a research facility and we

Re: [Histonet] Reticulin Stain

Gomori's René On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet wrote: Does anyone out there still do the retic by hand?  If so, can you share which procedure you use?  Thanks

Re: [Histonet] curiosity may kill me

I always used Auramine at room temperature to identify TB bacilli in tissue sections with fluorescence filter, never used heat and the results were as expected. Bancroft is the one describing the procedure using heat for  auramine/rhodamine procedure but auramine alone at room temperature is

Re: [Histonet] Certification

Your tech has an "above deserved" expectation.How would you even consider promoting somebody who is not qualified to even be HT certified to Lead HT/Coordinator?This is disrespectful for those who reversing that position have been unable to achieve it.It speaks volumes about your tech

Re: [Histonet] How to get your stains more vibrant when cutting at 2μm?

Angela:"Pale" results are the trade-off for great quality very thin "2 µm" sections but you can always improve intensity somewhat .1- your "regressive" stain, if it is "modern Harris" has the inherent problem of lacking mercury chloride and it is little you can do about. Perhaps if you use

Re: [Histonet] Fat or Breast & Lipoma Processing Schedule Sought

You can find all my xylene-free processing schedules at the HistoNet archives. They use 2-propanol → mineral oil → paraffin.René  On Saturday, February 11, 2017 9:44 AM, ian bernard via Histonet wrote: We use Safe Clear 11 (a Xylene sub) as the

Re: [Histonet] hand staining immunos

You do not have to "babysit" the procedure for it has well defined/timed steps. You just need a timer and check the slides when required. As to procedural "dos & donts" try to get a copy of the DAKO IHC manual.René On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet

Re: [Histonet] Releasing of Patient Tissue

Never mind what other people do, just ask your legal department what to do because this may involve legal consequences.René  On Wednesday, January 18, 2017 10:47 AM, Vanessa Keeton via Histonet wrote: Good Morning All! I was wondering what

Re: [Histonet] Placing formalin on specimens

Usually you do not pour 10%NBF onto a specimen; you place the specimen onto a container/vial with the 10%NBF.René On Tuesday, August 16, 2016 9:29 AM, Mike Pence via Histonet wrote: I know this might sound a bit crazy, but does anyone have a written

Re: [Histonet] Antibodies storage tips

I used to have several compartmented plastic alphabetized boxes with enough empty spaces to accommodate "new arrivals". If the spaces were used-up I just added a new box. Since they occupied several shelves, each shelf had the lettering identifying the boxes in each one.René On Tuesday,

Re: [Histonet] oil red in WAT frozen samples

Apply gently heated water on the sections in a way that the gelatin is washed out.René On Tuesday, August 16, 2016 4:48 AM, Monica Aguilera via Histonet wrote: Dears, I was wondering if some of you might have experience in the following: We have

Re: [Histonet] Query on authorship

Jorge:The first thing is to be absolutely sure the data is worth publishing and that the results have scientific relevance.If this is the case and both you and the other contributor agree I think the data should be published.In no way you should eliminate the data obtained by the other

Re: [Histonet] Modified Movat's Pentachrome stain

Once you start substituting things in an original recipe, the outcome cannot be expected to be what the original recipe was supposed to deliver.Iodine crystals cannot be substituted by Lugol because, besides the iodine also contains its salts. and alcohol. They are two completely different

Re: [Histonet] Modified Movat's Pentachrome stain

Do you have any contacts at any old histology lab, i.e., one that has been in operation for more than 50 years? You may find there iodine crystals and ask for a few grams. I used to have a 500 g bottle at my lab (which began in 1947).René On Thursday, September 1, 2016 10:39 AM, Angela

Re: [Histonet] Egg Whites and IHC Staining

Egg white (in Mueller's albumin) will always produce a "shadowy" staining with IH procedures y a dark background with IHC procedures.I suggest you use pork gelatin dissolves in the water of the water bath, use (+) charged slides and increase the drying time in the oven.Also sectioning those

Re: [Histonet] Quantifiable evaluation criteria

1- Make a list of ALL the tasks you delegate on this "Lead Histo"2- Quantify each tasks, i.t. give a "numeric weight" to each  of a maximum 100 points.3- Keep track of how the "Lead Histo" performs in each and DISCUSS your evaluation with the "Histo Lead" quarterly. This will allow the "Lead

Re: [Histonet] I guess I've found it at last

Yes, I received it.Most probably it is a disguised "junk/spam" advertisement.Just in case do NOT open it.René On Wednesday, August 24, 2016 10:58 AM, "Macke, Gail via Histonet" wrote: Histonet, Received this today. What is this? Can you look into

Re: [Histonet] Buffered formalin substitution

Julio:Unfortunately NBF is the OVERALL best fixative there is. ANY substitute will be good for some things and not that good for others. Under those circumstances what to do? Simply use LESS amounts of formalin, do it safely keeping to a minimum its exposure.Under separate cover I am sending

[Histonet] Fw: Dr. Matsionis

Hi colleagues:I have just received the sad news that the prestigious Russian histopathologist Prof. Alexander Matsionis passed away (see included message).Although his name is almost unknown in our field he always was extremely enthusiast about new histopathology procedures and helped

Re: [Histonet] Weigert's hematoxylin for IHC

Once you finish the IHC procedure, the DAB reaction is very stable and you can use Weigert's or any other iron hematoxylin.René On Tuesday, November 22, 2016 5:29 PM, Esther C Peters via Histonet wrote: Could someone advise me on whether Weigert's

Re: [Histonet] Storage temps

I do not know of anything published other than CAP "requirements" (unsubstantiated)René On Wednesday, November 23, 2016 9:50 AM, "Richardson, Pam K via Histonet" wrote: Does anyone know if there is a published acceptable range for storage of tissue

Re: [Histonet] Maximow Method

Tyrone:A.A.Maximow's Azur-eosin, etc staining produces wonderful results but this, and many very old procedures, essentially rest on the use of mercury salts which produce special chemical compounds with tissue components.Any, and I mean any, deviation from the original procedure will not

Re: [Histonet] new lab

If you already saw the drawings for the designated area, is it not too late now to make changes?If you think you can give input, my only suggestion is that you set your working areas in a way that they follow the workflow and ideally should be close to the surgery suits.René On Thursday,

Re: [Histonet] Removing Tissue From Tape

I think your best option is to manually re-coverslip to the slide.René On Sunday, October 16, 2016 10:00 AM, Pamela Marcum via Histonet wrote: Good Morning,   Although the laboratory stopped using tape years ago we are still facing issues with

Re: [Histonet] Slide and Block Storage

 We used to keep our blocks on-site during 9 years. During January of each year we disposed off 1 year worth of blocks (the oldest) and only kept those deemed  by the pathologists as interesting for our residents' training program.Regarding slides, in 2001 we had 56 years of slides on-site and

Re: [Histonet] Staining issues

Improper staining at the center and falling sections are typical consequences of poor fixation/infiltration.If you have changed nothing proceduraly, what about somebody "new" grossing and preparing thicker tissue slices?René On Wednesday, December 28, 2016 8:34 AM, Charles Riley via

Re: [Histonet] Embedding station

"Open" your options and try Sakura.René On Tuesday, March 21, 2017 3:54 PM, "Flynn, Evelyn via Histonet" wrote: Hello all,   Our laboratory is purchasing a new paraffin embedding station.  We are considering a Leica Arcadia or a Thermo HistoStar

Re: [Histonet] IHC billing on archived case

You should treat it as a new request.René On Wednesday, March 22, 2017 11:25 AM, "Haines, Beth via Histonet" wrote: Hello all, After some discussion on IHC billing, I have been asked to verify accepted billing practice for the following situation: An

Re: [Histonet] solvent recyclers

B/R recyclersRené On Thursday, March 23, 2017 11:15 AM, Lauren Sweeney via Histonet wrote: Hi everyone, We are looking into getting a new solvent recycler to recycle our xylene and alcohol, any recommendations? Thanks!

Re: [Histonet] low signal for long post fixation

Perhaps your only solution is to increase HIARRené On Thursday, March 23, 2017 9:39 AM, Mariela Chertoff via Histonet wrote: Dear all, I have mouse brain tissue perfused with PFA 4% and post fixed in formol 10% for periods between 2 month and 1 year.

Re: [Histonet] need help staining 120um human whole brain sections!

You have a special project → special tasks so your approach has to be equally special.Large brain sections are usually stained while floating but for IH with different and successive steps requiring very expensive reagents, floating sections is not well suited.You should affix the sections to

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