Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Lik Wee Lee
Furthermore, I also did: C:\Inetpub\wwwroot\ISB\data\17mixc:\Inetpub\tpp-bin\Tandem2XML c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.pep.xml but if I tried to browse file in petunia and click on [PepXML],

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Jimmy Eng
I suggest you start debugging the problem one step at a time. First confirm that your Tandem search actually ran by looking at contents of the .tandem file. Next, check contents of tandem.pep.xml. Do both of these files look like they have peptide IDs in them? On Wed, Mar 10, 2010 at 2:04 PM,

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Lik Wee Lee
Hi Jimmy, Yes, I also checked the .tandem file there were peptides with low expect score. I also looked at the .pep.xml file and checked that the scans, hyperscore, nextscore, bscore, ... matched the .tandem file. I am puzzled why the run_in doesn't work and wondering if this is affecting the

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Brian Pratt
I think the fact that run_in doesn't work indicates some kind of system level problem that's going to make everything else flaky. Perl shouldn't be an issue, at least at this point. So c:\inetpub\tpp-bin\run_in c:\; dir doesn't work for you? What do you see when you type this into your browser

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Lik Wee Lee
Hi Brian, On Wed, Mar 10, 2010 at 3:34 PM, Brian Pratt brian.pr...@insilicos.comwrote: I think the fact that run_in doesn't work indicates some kind of system level problem that's going to make everything else flaky. Perl shouldn't be an issue, at least at this point. So

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Brian Pratt
Did you have a working TPP installation before? On Wed, Mar 10, 2010 at 3:54 PM, Lik Wee Lee l...@systemsbiology.orgwrote: Hi Brian, On Wed, Mar 10, 2010 at 3:34 PM, Brian Pratt brian.pr...@insilicos.comwrote: I think the fact that run_in doesn't work indicates some kind of system level

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Brian Pratt
ah, wait, I do see one weird thing: WEBSERVER_ROOT = c:\Inetpub\wwwroot;c:\Inetpub\wwwroot change that environment variable to WEBSERVER_ROOT = c:\Inetpub\wwwroot and see how it goes. On Wed, Mar 10, 2010 at 4:18 PM, Lik Wee Lee l...@systemsbiology.orgwrote: Nope. I installed it recently

Re: [spctools-discuss] xtandem search didn't run through Petunia

2010-03-10 Thread Lik Wee Lee
Thanks!!. It is working normally now...phew. Lik Wee On Wed, Mar 10, 2010 at 4:46 PM, Brian Pratt brian.pr...@insilicos.comwrote: It's a windows thing: ControlPanel|System|Advanced|EnvironmentVariables Good luck! Brian On Wed, Mar 10, 2010 at 4:33 PM, Lik Wee Lee