Hi Brian,

On Wed, Mar 10, 2010 at 3:34 PM, Brian Pratt <[email protected]>wrote:

> I think the fact that run_in doesn't work indicates some kind of system
> level problem that's going to make everything else flaky.  Perl shouldn't be
> an issue, at least at this point.
>
> So "c:\inetpub\tpp-bin\run_in c:\; dir" doesn't work for you?
>

I still get  command "dir" failed: Unknown error

>
> What do you see when you type this into your browser address bar?
> http://localhost/tpp-bin/check_env.pl
>
>

SCRIPT_NAME = /tpp-bin/check_env.pl
SERVER_NAME = localhost
HTTP_ACCEPT_ENCODING = gzip,deflate
HTTP_CONNECTION = keep-alive
REQUEST_METHOD = GET
SYSTEMROOT = C:\WINDOWS
HTTP_ACCEPT =
text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8
SCRIPT_FILENAME = C:/Inetpub/tpp-bin/check_env.pl
COMSPEC = C:\WINDOWS\system32\cmd.exe
SERVER_SOFTWARE = Apache/2.2.8 (Win32)
HTTP_ACCEPT_CHARSET = ISO-8859-1,utf-8;q=0.7,*;q=0.7
WINDIR = C:\WINDOWS
QUERY_STRING =
REMOTE_PORT = 3227
PATHEXT = .COM;.EXE;.BAT;.CMD;.VBS;.VBE;.JS;.JSE;.WSF;.WSH
HTTP_USER_AGENT = Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US;
rv:1.9.0.18) Gecko/2010020220 Firefox/3.0.18 (.NET CLR 3.5.30729)
SERVER_PORT = 80
SERVER_SIGNATURE =
HTTP_ACCEPT_LANGUAGE = en-us,en;q=0.5
HTTP_COOKIE = TPPSession=TYBEEDEHS:guest:249069786675ffea98006f8c1b444fa1
REMOTE_ADDR = 127.0.0.1
WEBSERVER_ROOT = c:\Inetpub\wwwroot;c:\Inetpub\wwwroot
HTTP_KEEP_ALIVE = 300
SERVER_PROTOCOL = HTTP/1.1
PATH = C:\Inetpub\tpp-bin;C:\Xcalibur\system\programs;C:\Program
Files\Thermo\Foundation;C:\Perl\site\bin;C:\Perl\bin;C:\WINDOWS\system32;C:\WINDOWS;C:\WINDOWS\System32\Wbem;C:\Program
Files\Common Files\Adaptec Shared\System;C:\Program Files\Analyst\bin
REQUEST_URI = /tpp-bin/check_env.pl
GATEWAY_INTERFACE = CGI/1.1
SERVER_ADDR = 127.0.0.1
DOCUMENT_ROOT = C:/Program Files/Apache Software Foundation/Apache2.2/htdocs
HTTP_HOST = localhost

Thanks for your help,
Lik Wee


> It should give you a bunch of info including the PATH setting for your
> webserver.
>
> On Wed, Mar 10, 2010 at 2:47 PM, Lik Wee Lee <[email protected]>wrote:
>
>> Hi Jimmy,
>>
>> Yes, I also checked the .tandem file there were peptides with low expect
>> score.
>> I also looked at the .pep.xml file and checked that the scans, hyperscore,
>> nextscore, bscore, ... matched the .tandem file.
>>
>> I am puzzled why the run_in doesn't work and wondering if this is
>> affecting
>> the xinteract command as well. any ideas?
>>
>> Lik Wee
>>
>>
>> On Wed, Mar 10, 2010 at 2:20 PM, Jimmy Eng <[email protected]> wrote:
>>
>>> I suggest you start debugging the problem one step at a time.
>>> First confirm that your Tandem search actually ran by looking at
>>> contents of the .tandem file.
>>> Next, check contents of tandem.pep.xml.
>>> Do both of these files look like they have peptide IDs in them?
>>>
>>>
>>>
>>> On Wed, Mar 10, 2010 at 2:04 PM, Lik Wee Lee <[email protected]>
>>> wrote:
>>> > Furthermore, I also did:
>>> > C:\Inetpub\wwwroot\ISB\data\17mix>c:\Inetpub\tpp-bin\Tandem2XML
>>> > c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem
>>> >
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.pep.xml
>>> >
>>> > but if I tried to browse file in petunia and click on [PepXML],
>>> > it opens a new window:
>>> >
>>> http://localhost/tpp-bin/PepXMLViewer.cgi?xmlFileName=c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.pep.xml
>>> >
>>> > but with the message:
>>> >
>>> > error:
>>> > can't open html
>>> >
>>> templatec:/Inetpub/wwwroot;c:/Inetpub/wwwroot/ISB/html/PepXMLViewer.html
>>> >
>>> > I also had problem with xinteract:
>>> >
>>> > C:\Inetpub\wwwroot\ISB\data\17mix\tandem>c:\Inetpub\tpp-bin\xinteract
>>> > -Ninteract.pep.xml -p0.05 -l7 -O
>>> >
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.pep.xml
>>> >
>>> > c:\Inetpub\tpp-bin\xinteract (TPP v4.3 JETSTREAM rev 1, Build
>>> 200909091257
>>> > (MinGW))
>>> >
>>> > running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml"
>>> > "c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tand
>>> > em.pep.xml" -L"7""
>>> >
>>> > command "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml"
>>> > "c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tande
>>> > m.pep.xml" -L"7"" failed: Unknown error
>>> >
>>> > command "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml"
>>> > "c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tande
>>> > m.pep.xml" -L"7"" exited with non-zero exit code: -1
>>> > QUIT - the job is incomplete
>>> >
>>> >
>>> >
>>> > Is this an installation issue? Any ideas?
>>> >
>>> > Thanks,
>>> > Lik Wee
>>> >
>>> > On Wed, Mar 10, 2010 at 1:53 PM, Lik Wee Lee <[email protected]>
>>> > wrote:
>>> >>
>>> >> Hi Brian,
>>> >>
>>> >> The full path still gives command "..." failed: unknown error.
>>> >>
>>> >> I tried "run_in c:" and didn't get any output.
>>> >>
>>> >> But if I try "run_in c:\; dir", I get:
>>> >> command "dir" failed: Unknown error.
>>> >>
>>> >> Could it be I previously had installed Perl 5.10? I uninstalled it
>>> >> and then installed Perl 5.8.9 followed by tpp 4.3.1.
>>> >>
>>> >> Lik Wee
>>> >>
>>> >> On Wed, Mar 10, 2010 at 1:34 PM, Brian Pratt <
>>> [email protected]>
>>> >> wrote:
>>> >>>
>>> >>> "run_in" is just a little program that runs one or more commands in
>>> the
>>> >>> indicated directory.  For example "run_in c:\foo; bar.exe; baz.exe"
>>> performs
>>> >>> "cd c:\foo" then runs "bar.exe" then "baz.exe".  It exists to help
>>> with
>>> >>> linux-oriented multipart command lines in the GUI, statements like
>>> "cd
>>> >>> c:foo; bar.exe; baz.exe" (we just swap "run_in" for "cd" on the
>>> windows
>>> >>> installation).
>>> >>>
>>> >>> Anyway, that's what it's for - why it doesn't work for you is a
>>> mystery.
>>> >>> Perhaps it's a path issue: how does the command line
>>> >>> c:\Inetpub\tpp-bin\run_in c:/Inetpub/wwwroot/ISB/data/17mix/tandem;
>>> >>> c:\Inetpub\tpp-bin\tandem
>>> >>>
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.params
>>> >>>
>>> >>> work for you (that is, with full path to run_in specified)?
>>> >>>
>>> >>> On Wed, Mar 10, 2010 at 1:21 PM, Lik Wee Lee <
>>> [email protected]>
>>> >>> wrote:
>>> >>>>
>>> >>>> Hi,
>>> >>>>
>>> >>>> When I tried to do a xtandem search in the TPP petunia interface,
>>> >>>> I encountered the problem:
>>> >>>>
>>> >>>> # Commands for session TYBEEDEHS on Wed Mar 10 13:13:38 2010
>>> >>>> # BEGIN COMMAND BLOCK
>>> >>>> ###### BEGIN Command Execution ######
>>> >>>>
>>> >>>> [Wed Mar 10 13:13:38 2010] EXECUTING: run_in
>>> >>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem; c:\Inetpub\tpp-bin\tandem
>>> >>>>
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.params
>>> >>>> OUTPUT:
>>> >>>>
>>> >>>> command "c:\Inetpub\tpp-bin\tandem
>>> >>>>
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.params"
>>> >>>> failed: Unknown error
>>> >>>>
>>> >>>>
>>> >>>>
>>> >>>>
>>> >>>> END OUTPUT
>>> >>>> RETURN CODE:65280
>>> >>>> ###### End Command Execution ######
>>> >>>> # All finished at Wed Mar 10 13:13:38 2010
>>> >>>> # END COMMAND BLOCK
>>> >>>>
>>> >>>> Does anyone have any ideas why this occur?
>>> >>>> However, the search was performed successfully if I did it by
>>> command
>>> >>>> line:
>>> >>>>
>>> >>>> c:\Inetpub\tpp-bin\tandem
>>> >>>>
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.params
>>> >>>>
>>> >>>> If I try
>>> >>>>
>>> >>>> run_in c:/Inetpub/wwwroot/ISB/data/17mix/tandem;
>>> >>>> c:\Inetpub\tpp-bin\tandem
>>> >>>>
>>> c:/Inetpub/wwwroot/ISB/data/17mix/tandem/OR20091211_18mix_01.tandem.params
>>> >>>>
>>> >>>> it says command failed: Unknown error.
>>> >>>>
>>> >>>> I have ActivePerl 5.8.9 build 827 and TPP version 4.3.1 installed on
>>> >>>> Windows XP SP3.
>>> >>>>
>>> >>>> Thanks,
>>> >>>> Lik Wee
>>> >>>>
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