Hi,
Can anyone shed some light on why the "Mixture model quality test
failed for charge (2+) and (3+)". They are the majority of my spectra
and I get good hyperscores especially from the 3+ spectra prior to
xinteract command. I do not feel my data is bad, but apparently
peptide prophet cannot make anything out of it...

I'm using TPP 4.4.0 on Mac (gui and command line), but I can reproduce
the error on the windows TPP 4.4.0. (also gui and command line).

Thanks,

Bjorn

PeptideProphet  (TPP v4.4 VUVUZELA rev 0, Build 201008200839 (linux))
akel...@isb
read in 695 1+, 7797 2+, 9641 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.
Initialising statistical models ...
negmean = -0.94065
negmean = -1.41046
negmean = -1.64247
negmean = -0.2
negmean = -0.2
negmean = -0.2
negmean = -0.2
Iterations: .........10.........20....
WARNING: Mixture model quality test failed for charge (2+).
WARNING: Mixture model quality test failed for charge (3+).
model complete after 25 iterations
command completed in 3 sec

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