The easiest way to add contaminants is to find the ones you want from your favorite source (IPI, UniProt, wherever) and manually copy them into your database file. If you open your database in a text viewer, you'll see it has a very simple format. Once you have your complete database, you can use the Decoy Databases tool in the TPP to add reverse sequence decoys (this is especially easy to do in Petunia). When you create your appended database, be sure not to leave any blank lines between entries, otherwise entries will get deleted when you create your decoy database.
On Jan 27, 8:36 pm, 钟传奇 <[email protected]> wrote: > Hi guys, > I want to implement a database search, but I do not know how to create > a composite database containing forward and decoy sequences with > contaminants and trypsin. I know a software named > "DecoyDatabaseBuilder" that can create a composite database from a IPI > database, but that how to include the contaminants and trypsin > sequences into the composite database troubles me. > Could anyone help me? > Thanks. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
