Thanks, Kristian.
I will try.

On Sat, Jan 29, 2011 at 2:36 AM, Kristian <[email protected]> wrote:
> The easiest way to add contaminants is to find the ones you want from
> your favorite source (IPI, UniProt, wherever) and manually copy them
> into your database file.  If you open your database in a text viewer,
> you'll see it has a very simple format.  Once you have your complete
> database, you can use the Decoy Databases tool in the TPP to add
> reverse sequence decoys (this is especially easy to do in Petunia).
> When you create your appended database, be sure not to leave any blank
> lines between entries, otherwise entries will get deleted when you
> create your decoy database.
>
> On Jan 27, 8:36 pm, 钟传奇 <[email protected]> wrote:
>> Hi guys,
>> I want to implement a database search, but I do not know how to create
>> a composite database containing forward and decoy sequences with
>> contaminants and trypsin. I know a software named
>> "DecoyDatabaseBuilder" that can create a composite database from a IPI
>> database, but that how to include the contaminants and trypsin
>> sequences into the composite database troubles me.
>> Could anyone help me?
>> Thanks.
>
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