I have a batch script which works pretty well, but continuously
produces popup consoles while Sequest is running. Does anyone know a
possible solution to this?
___________________________________________________________________________
set /p filename= Please, type the name of your .RAW file (excluding
the extension):
set /p paramfile= Type the name of your parameter file (excluding the
extension):

ReAdW -v --mzXML %filename%.RAW

runsearch -p%paramfile%.params -P15 %filename%.mzXML
sequest -P%paramfile%.params -Dxargs

out2XML.exe %filename% 1 -P%paramfile%.params -Etrypsin

xinteract  -N%filename%.pep.xml -p0.05 -l7 -O %filename%.pep.xml

proteinprophet %filename%.pep.xml %filename%.prot.xml
_____________________________________________________________________________________

Adjust each line to specify where exactly your files are and save it
as a batch script.
As I said it works well, but it produces annoying popup as the Sequest
search is running.

-Ned




On Aug 3, 2:38 pm, Joseph Slagel <[email protected]>
wrote:
> In theory it should work under nmake -- but I won't unleash this on anyone
> without extensively testing it first.  Hopefully someone else has a
> solution, otherwise you could piece together a .bat file pretty easily by
> looking at the commands issued in petunia.
>
> -Joe
>
> On Wed, Aug 3, 2011 at 11:15 AM, John Bryant <[email protected]>wrote:
>
>
>
>
>
>
>
> > In Windows 7
>
> > On Aug 3, 1:31 pm, Joseph Slagel <[email protected]>
> > wrote:
> > > Windows or Unix?  A long time ago I played around with a Makefile that
> > would
> > > run the process, and had the added benefit of "knowing" when to rerun
> > > certain parts when files changed (such as edits to parameters).
>
> > > -Joe
>
> > > On Wed, Aug 3, 2011 at 7:21 AM, John Bryant <[email protected]>
> > wrote:
> > > > Does anyone have a script that automatically runs the entire TPP
> > > > process from converting .RAW files from the mass spectrometer to
> > > > identify the proteins using protein prophet?
> > > > I have used the TPP for a while and I don't like having to wait for
> > > > each step to finish before I manually initiate the next step of the
> > > > process.
>
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