Hi SPC-group

I have recently installed TPP v 4.5 and the program works fine with
the test-dataset I got from the TPP-course held as a HUPO 2011
Satellite Workshop.

I got a wiff-file of my sample from the AB-Sciex demo-lab and now want
to test it with TPP. However when I want to convert the wiff-file to
mzML (In the Analysis Pipeline) it does not work and the mzML file is
therefore not created. I have copied the command line from TPP and
pasted it in the bottom of this mail.

The command is successful, but the file is unreadable, so I don’t know
what TPP is missing. Have you seen this message before and know what
is wrong?

I have installed an older version of an Analyst library on my
computer, but I don’t know if it fits the generated wiff-file from the
demo-lab. Could this be the reason why the mzML- conversion does not
work? If yes, do you know where I could get a free demo version of
more current Analyst library just to test the sample?

Best regards Mia Larsen

Copied from TPP:
  c:/Inetpub/wwwroot/ISB/data/potato/formats/Stensballe_PotatoGS.mzML
(file not found: not created or deleted?)

 Command 1 [Thu Oct 6 12:09:02 2011]               [ Show / Hide ]
c:\Inetpub\tpp-bin\msconvert c:/Inetpub/wwwroot/ISB/data/potato/
formats/Stensballe_PotatoGS.wiff -v --mzML -o c:/Inetpub/wwwroot/ISB/
data/potato/formats

format: mzML (Precision_64 [ 1000514:Precision_64
1000515:Precision_32 ], ByteOrder_LittleEndian, Compression_None)
indexed="true"
outputPath: c:/Inetpub/wwwroot/ISB/data/potato/formats
extension: .mzML
contactFilename:

filters:

filenames:
  c:/Inetpub/wwwroot/ISB/data/potato/formats/Stensballe_PotatoGS.wiff

processing file: c:/Inetpub/wwwroot/ISB/data/potato/formats/
Stensballe_PotatoGS.wiff
writing output file: c:/Inetpub/wwwroot/ISB/data/potato/formats/
Stensballe_PotatoGS-Potato Gel Slice 2.mzML
54273/54273
1/1
Command Successful

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