You pipeline is missing the InteractParser step.  Once you have a pep.xml
file from Tandem you can run "xinteract -ip -p0 "on it to generate
InteractParser->PeptideProphet->iProphet->ProteinProphet pipeline results.

-David


On Tue, Aug 13, 2013 at 10:16 AM, Rishika Bisaria
<[email protected]>wrote:

> Hi,
> I am able to run successfully the TPP pipeline on one of the mzXML
> file.But on using TPP on other mzXML file , ProteinProphet is throwing
> error stating that did you forgot to run PeptideProphet.
> I have crossed checked all the parameters , they are correct.I am using
> the latest version of TPP on windows and running it using commandline.
>
> 1.tandem input.xml
> 2.Tandem2XML output.2013_07_24_13_24_28.t.xml
> output.2013_07_24_13_24_28.pep.xml
> 3.PeptideProphetParser output.2013_07_24_13_24_28.pep.xml MINPROB=0.2
> 4.ProteinProphet output.2013_07_24_13_24_28.pep.xml
>  c:\Inetpub\wwwroot\out1.prot.xml
>
> ****
>
> P.S. Everytime I am changing the parameters in input.xml file.I am using
> k-score function.
>
>
> Thanks,
> Rishika
>
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