Any reason you would see this other than Tandem search failed to generate
results?

Parsing search results "SP062512_062912_IP0201_AX01_23to24 (X! Tandem
(k-score))"...
  => Total of 0 hits.
  => Total of 0 decoy hits.
  => Total of 0 excluded hits.


On Tue, Aug 13, 2013 at 11:41 AM, Rishika Bisaria
<[email protected]>wrote:

> Hi,
> Thank you for the reply.
> Actually I want to follow this pipeline:
>
> Tandem->peptideprophet->q3new->proteinprophet->Q3proteinRatioParser.
>
> And also when I am using Xinteract , I am getting the below error:
>
> running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i interact.iproph.pep.xml"
> Analyzing interact.iproph.pep.xml ...
> Reading NSS model  ...
> Reading NRS model  ...
> Reading NSE model  ...
> Reading NSI model  ...
> Reading NSM model  ...
> Reading NSP model  ...
> Parsing search results "SP062512_062912_IP0201_AX01_23to24 (X! Tandem
> (k-score))"...
>   => Total of 0 hits.
>   => Total of 0 decoy hits.
>   => Total of 0 excluded hits.
> command completed in 1 sec
>
> running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I
> c:/xtandem/interact.iproph.pep.xml"
>
> command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I
> c:/xtandem/interact.iproph.pep.xml" failed: Unknown error
>
> command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I
> c:/xtandem/interact.iproph.pep.xml" exited with non-zero exit code:
> -1073741819
> QUIT - the job is incomplete
>
>
>
>
>
> On Tue, Aug 13, 2013 at 2:28 PM, David Shteynberg <
> [email protected]> wrote:
>
>> You pipeline is missing the InteractParser step.  Once you have a pep.xml
>> file from Tandem you can run "xinteract -ip -p0 "on it to generate
>> InteractParser->PeptideProphet->iProphet->ProteinProphet pipeline results.
>>
>> -David
>>
>>
>> On Tue, Aug 13, 2013 at 10:16 AM, Rishika Bisaria <
>> [email protected]> wrote:
>>
>>> Hi,
>>> I am able to run successfully the TPP pipeline on one of the mzXML
>>> file.But on using TPP on other mzXML file , ProteinProphet is throwing
>>> error stating that did you forgot to run PeptideProphet.
>>> I have crossed checked all the parameters , they are correct.I am using
>>> the latest version of TPP on windows and running it using commandline.
>>>
>>> 1.tandem input.xml
>>> 2.Tandem2XML output.2013_07_24_13_24_28.t.xml
>>> output.2013_07_24_13_24_28.pep.xml
>>> 3.PeptideProphetParser output.2013_07_24_13_24_28.pep.xml MINPROB=0.2
>>> 4.ProteinProphet output.2013_07_24_13_24_28.pep.xml
>>>  c:\Inetpub\wwwroot\out1.prot.xml
>>>
>>> ****
>>>
>>> P.S. Everytime I am changing the parameters in input.xml file.I am using
>>> k-score function.
>>>
>>>
>>> Thanks,
>>> Rishika
>>>
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