Hello David,

Sorry, i still found the same problem when i get the correct .dat format,
Could you please help test this file:
https://docs.google.com/file/d/0B-nLuoS-pq14TmdIX1k5ME52anc/edit?usp=sharing

I still get the same error message:

Error: 144596 identity scores, but 95523 ion scores read

command "c:\Inetpub\tpp-bin\Mascot2XML c:/Inetpub/wwwroot/ISB/data/CL1-5.dat 
-Dc:/Inetpub/wwwroot/ISB/data/SwissProt_57.8.fasta -Etrypsin" failed: Operation 
not permitted

*Command FAILED*

RETURN CODE:256
Many thanks!!

Peter



On Wednesday, October 23, 2013 1:09:31 PM UTC+8, zion wrote:
>
> Hello David,
>
> The problem now is solved.
>
> Many thanks.
>
> Peter.
>
> On Wednesday, October 23, 2013 7:02:18 AM UTC+8, David Shteynberg wrote:
>>
>> Hello again,
>>
>> I was able to replicate your problem on my computers.  It appears that 
>> your file is corrupted in at least one place.  The first location I saw the 
>> corrupted data was on line: 1644096.  There may be other corrupted parts. 
>>  Please see try copying your file over from the Mascot server again in case 
>> the corruption occurred during file transfer.
>>
>> Cheers,
>> -David 
>>
>>
>> On Mon, Oct 21, 2013 at 5:18 PM, zion <[email protected]> wrote:
>>
>>> Hi David:
>>>
>>> I post my data on : 
>>>
>>> https://docs.google.com/file/d/0B-nLuoS-pq14Yl9NWFZoMnZtbmM/edit?usp=sharing
>>>
>>> Thanks a lot!!
>>>
>>> Peter
>>>
>>>
>>>
>>> On Tuesday, October 22, 2013 12:24:55 AM UTC+8, David Shteynberg wrote:
>>>
>>>> Actually the problem is this:  Error: 146354 identity scores, but 
>>>> 96591 ion scores read
>>>>
>>>>
>>>> Is your dat file complete?  Can you post your dat file somewhere?
>>>>
>>>> -David
>>>>
>>>>
>>>> On Mon, Oct 21, 2013 at 9:22 AM, David Shteynberg <David.Sh...@**
>>>> systemsbiology.org> wrote:
>>>>
>>>>> Hi Peter,
>>>>>
>>>>>
>>>>> Can you see you if have the same error on a file that does not contain 
>>>>> '-' in it's file name? 
>>>>>
>>>>> Thanks,
>>>>> -David
>>>>>
>>>>>
>>>>> On Mon, Oct 21, 2013 at 6:26 AM, zion <[email protected]> wrote:
>>>>>
>>>>>>  Dear All:
>>>>>>
>>>>>> I have installed TPP4.6.3,
>>>>>> and when converting .dat to .pepxml,
>>>>>> there is a error message like this:
>>>>>>
>>>>>> variable modification 1, delta mass 45.9877 for Methylthio (C):
>>>>>>  residues: C
>>>>>>  nterm: 0
>>>>>>  cterm: 0
>>>>>>  protterm: 0
>>>>>>
>>>>>> variable modification 2, delta mass 15.9949 for Oxidation (M):
>>>>>>  residues: M
>>>>>>  nterm: 0
>>>>>>  cterm: 0
>>>>>>  protterm: 0
>>>>>>
>>>>>> Error: 146354 identity scores, but 96591 ion scores read
>>>>>>
>>>>>> command "c:\Inetpub\tpp-bin\Mascot2XML 
>>>>>> c:/Inetpub/wwwroot/ISB/data/**CL1-0.dat 
>>>>>> -Dc:/Inetpub/wwwroot/ISB/data/**SwissProt_57.8.fasta -Etrypsin" failed: 
>>>>>> Operation not permitted
>>>>>>
>>>>>>
>>>>>>
>>>>>> *Command FAILED*
>>>>>> RETURN CODE:256
>>>>>>
>>>>>> CAN anyone kindly provide solutions to this problem?
>>>>>>
>>>>>> Thanks a lot!!
>>>>>>
>>>>>> Peter
>>>>>>  
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