Does that CA have a metal center (I think they all do)? If so, doesn't the
citrate compete for the metal?
JPK
On Mon, Feb 4, 2013 at 2:25 PM, Roger Rowlett rrowl...@colgate.edu wrote:
Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium
citrate/0.1 M TrisCl pH 8.5 at 10
So, I know I say this every time I post on this board, but here it goes
again.
I'm at an undergrad only school, and every 2 years I teach a class in
protein crystallography. This year I'm being super ambitious, and I'm
going to take a class of 16 to the synchrotron for data collection.
Hi David
try going back to the one that started it all,* myoglobin, a recipe
is at
http://www.rigaku.com/products/protein/recipes
(* feel free to argue about this)
On 4 Feb 2013, at Mon4 Feb 16:03, David Roberts wrote:
So, I know I say this every time I post on this board, but
Dear Powell,
Isn't it there a way to data mine the PDB or the other repository source
for the time/duration/days of the crystals obtained.
Dr. Jayashankar Selvadurai
Hannover
Germany
On Mon, Feb 4, 2013 at 5:10 PM, Harry Powell ha...@mrc-lmb.cam.ac.ukwrote:
Hi David
try going back to the
I don't know. Is there?
On 4 Feb 2013, at Mon4 Feb 16:15, Jayashankar wrote:
Dear Powell,
Isn't it there a way to data mine the PDB or the other repository
source for the time/duration/days of the crystals obtained.
Dr. Jayashankar Selvadurai
Hannover
Germany
On Mon, Feb 4, 2013 at
Hi David,
You could try the Glucose Isomerase supplied by Hampton. It crystallizes
under a number of conditions, details of which you can find in their manual.
http://hamptonresearch.com/product_detail.aspx?cid=28sid=56pid=56
Ganesh
Le 04/02/13 17:03, David Roberts a écrit :
So, I know I
/home/dr-anthony-addlagatta-1
-- Original Message ---
From: Harry Powell ha...@mrc-lmb.cam.ac.uk
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, 4 Feb 2013 16:10:54 +
Subject: Re: [ccp4bb] need some suggestions for crystallization
***
This message has been scanned
My suggestions would be to look up citations for thaumatin and glucose
isomerase. If I remember correctly, both of them form well diffracting
crystals within a short period of time. I think you can also buy the
purified protein from a vendor. Perhaps you could also try the good old
lysozyme.
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
Dear Dave,
many methods articles mention a small set of commonly used proteins. E.g.
Mueller et al, Optimal fine phi-slicing for single-photon-counting
pixel detectors, Acta Cryst D68, p42-56 list Insulin, Lysozyme,
Thaumatin, and Thermolysin;
Nanao
Building, Rm 310
4500 San Pablo Road
Jacksonville, FL 32224
(904) 953-6372
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of David
Roberts
Sent: Monday, February 04, 2013 11:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] need some suggestions
I second that. Gently swirling the bottle (very important) before
pipetting a few microliters (say 100 microliters or whatever).
Dialyse overnight vs 10 mM HEPES 2 mM MgCl2 ph7, then get the protein
concentration to 10 mg/ml. Set up the drops (sitting drops) versus the
same buffer with 30%
Human carbonic anhdyrase II can be easily crystallized from 1.3 M sodium
citrate/0.1 M TrisCl pH 8.5 at 10 mg/mL protein concentration. Crystals
are P21 and easily diffract to beyond 2.0 A on a home source. We
cryopreserve in ML + 30% glucose. Sulfonamide ligands are easy to soak
into the
are
interested I can send you more details about the protocol.
Mike
- Original Message -
From: David Roberts drobe...@depauw.edu
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, February 4, 2013 8:03:09 AM GMT -08:00 US/Canada Pacific
Subject: [ccp4bb] need some suggestions for crystallization
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