Sorry if this is an insulting question, but did you store it in enough glycerol
to prevent it from freezing? Proteins don't like freeze/thaw cycles,
especially slow ones.
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of jai mohan
Dear Jaimohan,
With high amount of purified protein (like obtained in recombinant expression)
they are likely to form higher oligomers if they have intrinsic property of
association. With time the content of oligomers do increase. In SDS-PAGE even,
you will see a faint band of oligomer. This
Hello Jaimohan,
Is it SDS-PAGE gel or Native PAGE gel ?. Theoretically SDS-PAGE is
supposed to show monomers and Native PAGE oligomers
On Tue, Jun 27, 2017 at 8:27 AM jai mohan <
0cab66323371-dmarc-requ...@jiscmail.ac.uk> wrote:
> Dear all,
> I am working on a Red Fluorescent
, 2017 8:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Separating Monomers and Dimers
it was not stable for frozen storage. if necessary，using protein fresh without
frozen
发自网易邮箱大师
在2017年06月27日 20:22，jai
mohan<mailto:0cab66323371-dmarc-requ...@jiscmail.ac.uk> 写道：
Dear all,
I am w
it was not stable for frozen storage. if necessary，using protein fresh without
frozen
发自网易邮箱大师
在2017年06月27日 20:22，jai mohan 写道：
Dear all,
I am working on a Red Fluorescent Protein (His-Tag) molecular weight around
27kDa. After purification I ran a SDS page, the band at 27kDa confirms the
