Hi folks
Any of you who are considering attending ECM27 in Bergen may be interested in
some of the sessions that have been arranged at this Software Fayre.
The full list of Microsymposia at ECM27 should be available in the next couple
of days
From: Martin Lutz m.l...@uu.nl
Date: 10 May 2012
Is there a fix for this? It has been a perennial request for some time..
eleanor
On 9 May 2012 10:53, sonali dhindwal sonali11dhind...@yahoo.co.in wrote:
Dear All,
I want to take the backup of all my data which i have refined using CCP4.
Can you please guide if I copy all the ccp4 data
Dear All,
This question sounds simple but I dont know the answer.I was preparing a 24
well crystal screen. When I try to use 10 mM ZnSO4 with HEPES (pH 7.6) buffer
it precipitates. I tried both ZnCl2 and Zn acetate the effect is same. I dont
know why this Zn in not compatible with
Try adding water first, so that you are not mixing concentrated Zn with
concentrated HEPES. Also it depends what else is in your cocktail.
JPK
On Fri, May 11, 2012 at 11:26 AM, Rajesh Kumar ccp4...@hotmail.com wrote:
Dear All,
This question sounds simple but I dont know the answer.
I was
On 05/11/12 12:26, Rajesh Kumar wrote:
Dear All,
This question sounds simple but I dont know the answer.
I was preparing a 24 well crystal screen. When I try to use 10 mM
ZnSO4 with HEPES (pH 7.6) buffer it precipitates. I tried both ZnCl2
and Zn acetate the effect is same.
I dont know why
Initial screen was 0.1 M Hepes 7.5 and 1.25 M LiSO4. I added ZnSO4 to HEPES and
immediately it precipitated.Just now I tried to add 10 mM ZnSO4 in to tube
after volume is made up and only with HEPES 100mM pH 7.5.It happens as earlier.
So is there any way I could use Zn with HEPES. I thought
If there is no cure , then fine.pH may not be the answer as it doesn't Happen
with TRIS buffer pH 7.6.Thanks to every oneRajesh
Date: Fri, 11 May 2012 12:35:45 -0400
From: dj...@cornell.edu
Subject: Re: [ccp4bb] zinc with HEPES
To: CCP4BB@JISCMAIL.AC.UK
There is no
pH is the culprit here
Like some already mentioned
change your pH to 6.4 and use a different buffer like cacodylate
or you can use Zinc acetate in water pH still would be 6.4
from your last mail
why would you add Zn to your initial hit condition
is there any rationale?
Padayatti
On Fri, May 11,
That is probably because you pH Tris with HCl rather than HEPES with NaOH.
The Ksp for Zn(OH)2 is 3x10^17 so the excess hydroxides are probably what
are killing your solution.
Cheers,
Katherine
On Fri, May 11, 2012 at 11:43 AM, Rajesh Kumar ccp4...@hotmail.com wrote:
If there is no cure ,
The rationale was to see if Zn could make differences in crystal morphology.
This is because the protein has CxxC and CxxH similar to a zinc finger
motif.All my efforts, additive screening, MMS, streaking, micro batch, hanging
drop, changing drop ratio, drop shape, did not help me to either
Just to make sure I understand pH correctly: isn't it true that the [OH-]
should always be the same at a given pH (by definition)?
JPK
On Fri, May 11, 2012 at 11:48 AM, Katherine Sippel
katherine.sip...@gmail.com wrote:
That is probably because you pH Tris with HCl rather than HEPES with
Almost assuredly you are getting zinc hydroxide precipitate. Zn(OH)2
will readily precipitate from solutions of alkaline Zn(II). Your problem
is compounded by the fact that HEPES is a non-coordinating buffer, so it
does not help solubilize the zinc ion. You might find a weakly
coordinating
Dear Patrick,
You along with others had made some suggestions last time. May be its a good
time to update.
With classical screening, I got a crystal like appearances/shower with HEPES
7.5 and LiSo4 1.5M. Trying to vary the pH of Hepes or using Tris and with
different conc of Lithium I could
mitegen loops might help, particularly micromesh...
JPK
On Fri, May 11, 2012 at 12:35 PM, Rajesh Kumar ccp4...@hotmail.com wrote:
Dear Patrick,
You along with others had made some suggestions last time. May be its a
good time to update.
With classical screening, I got a crystal like
It sounds as though microseeding worked very well, but the crystals are
still growing far too quickly.
Try diluting the protein and/or the reservoir solution to half the concs
you are using or lower.
To have enough protein you need larger drops, say 500 + 500 + 200 by hand
if you can't do it
Jacob Keller wrote:
Just to make sure I understand pH correctly: isn't it true that the
[OH-] should always be the same at a given pH (by definition)?
Maybe not by definition but by equilibration with [H+] to make water:
[H][OH]/[H2O] = Kw
[OH] = Kw[H2O]/[H]
if [W]
You are correct. My Friday frazzled brain is stuck in pharmacology mode so
I was thinking not of free hydroxide concentration but in terms of
potential exchangable groups being sequestered by the buffer. No more
posting on Friday. My apologies.
Katherine
On Fri, May 11, 2012 at 12:23 PM, Jacob
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