Did anyone suggest adding any known ligand? If your protein happens to
bind some compound/peptide/DNA/RNA/whatever, including that other partner
could dramatically change it's solution properties and be an easy fix for
your handling/formulation issues.
Good luck!
On Fri, Jul 14, 2017 at 6:33 AM,
Dear John,
I can't answer your questions with the limited information you supply, but
I would recommend contacting the company that sold you the detector. Most
probably that's the maker of your diffractometer. They'll be able to help
you out. If it's something that requires repair, they might
Dear John,
Having just seen Andreas's message regarding the best source of
support to address your enquiry, I have a further remark to make about
your instrument.
As this is a lab instrument, the Omega axis would be vertical,
and indeed the beam stop shadow (vertical on the top module)
Hi Chris,
In your Ni-NTA buffer, the total [Na+] could be greater than 530mM after
titration. Likely, your protein likes higher concentration salt for surface
charge stabilization. Adding Glycerol may further modify the charge
distribution and make the protein happy in the solution. For further
Regarding daftness, it seems that the detector is wider than tall, which should
improve the ratio of Lorentz-problematic reflections to good, fast-moving ones.
So I assume it was a choice between that and excluding some spots in the
gap--an appropriate calculation could be done to see whether
Thank you Gerard.
This seems to be a very valid point.
I myself cannot fully rule out the possibility that greater minds than mine
may present compelling arguments for installation with a horizontal gap
orientation.
Yet… taking your point, it is admittedly difficult for me to see any advantage
Thank you for the advice.
I had hoped to ascertain the frequency of this issue amongst the user base
and perhaps learn whether there is anything at all that we users can do to
reduce the likelihood of its reoccurrence.
I suspect that this is a rare issue and that it is likely an electronics
