[ccp4bb] positions for PhD students

TIGP program. Joseph Ho

[ccp4bb] refining anisotropy B factors on only a subset of atoms

Hello, I have a refined 1.8A structure that I wonder if I could squeeze out some anisotropy information. I did TLS refinement on the protein, and it helped my Rfree. But I would like to ask a biological question based on the thermal movement of only a few waters (7 total). In theory, that is

[ccp4bb] rastep r3d file for pymol

Hello, I would like to display thermal ellipsoids in pymol. What I did was using rastep to make a r3d file and open it in pymol. If I generate the r3d file with no extra options: rastep infile.pdb outfile.r3d, pymol can open it. But if I generate the file with rastep -auto -fancy1

Re: [ccp4bb] Removing a tight binding ligand

We often dialysis protein against charcoal to remove small molecules that tightly bind to protein. Meng-Chiao Joseph Ho, PhD Department of Biochemistry Albert Einstein College of Medicine Hi all, I am working with a substrate binding protein. The protein scavenges its endogenous ligand out

Re: [ccp4bb] Removing a tight binding ligand

It is just like the regular dialysis but the deserved buffer contains charcol powder. Meng-Chiao Joseph Ho How do you do this ? I have not heard of this, but I also never had to deal with getting rid of a ligand. However I would be interested to learn more about this method. Thanks

Re: [ccp4bb] phaser troubles

in the phaser with the ccp4 directory (for example, /your directory/ccp4-6.1.1/ccp4-6.1.1/ccp4i/bin/phaser/). Meng-Chiao Joseph Ho Postdoc Dept. of Biochemistry Albert Einstein College of Medicine On Mon, 2009-08-24 at 18:03 +0200, Sylvia Fanucchi wrote: Hi This is probably an obvious one but I’m

Re: [ccp4bb] Crystal rescue

Hi, Zhiyi: You can always put a layer of oil on the top of your drop when you open the coverslip. It will get you more time to mount the crystals. Good luck Meng-Chiao Ho On Tue, 2010-01-26 at 11:48 -0500, Zhiyi Wei wrote: I forgot to mention a phenomenon when I did crystal mounting. I found

[ccp4bb] Have everyone had a Scorpion Screen Builder or a Dragonfly screen optimizer?

Dear all: We are interested in purchasing either a Scorpion Screen Builder from ARI or a Dragonfly from TTP labtech for setting up the grid screen. I am wondering if anyone can share their personal experience or opinions with me. Your help/comments are highly appreciated. Joseph

[ccp4bb] postdoc/RA positions available at Academia Sinica

/MDTsai/ and http://www.ibc.sinica.edu.tw/ho We are looking forward to receiving your application! Joseph Ho

[ccp4bb] suggestion on protein crystallization optimization from phase separation

the Gelationous protein to new drop as shown in http://xray.bmc.uu.se/terese/tutorial3.html . Can you please share your experience with us? Thanks for your help. Joseph Ho

[ccp4bb] Protein or DNA crystals

Dear all: I would like to seek your opinion on our crystal hits. We are working on protein/dsDNA complex. By changing different protein and DNA (14-22bp) constructs, we recently got some hits from commercial screens using sitting drop vapor diffusion (very small xtals). The precipitant is PEG and

[ccp4bb] Tenure-tracked faculty - Institute of Biological Chemistry, Academia Sinica

and a microfocus protein crystallography beamlines now (http://tpsbl.nsrrc.org.tw/bd_page.aspx?lang=en=07A=1118). Most of scientific lectures are given in English and the grant application is also in English. Meng-Chiao Joseph Ho The following is our ads and you can find it at (https

Re: [ccp4bb] Strange Diffraction pattern! Protein/DNA complex or DNA alone crystal?

Dear Xiao and Hans: Thanks for your reply. We tried to index it but failed. Joseph On Mon, Mar 12, 2018 at 11:15 PM, Xiao Lei <xiaolei...@gmail.com> wrote: > did you try to index it? the cell dimensions may give you hint. > > On Mon, Mar 12, 2018 at 4:40 AM, Joseph Ho <sbdd