I have beautiful reasonably big crystals from a protein which diffract to 6A at the most.
I have tried adding additives, crystallizing in different methods (hanging, drops, sitting drops and microbatch under oil).
The crystals grow over night and contain high percent of water (they are very fragile).
I also tried different methods of dehydration (using different PEG's, MPD, glycerol and so on).
Currently I am trying to grow them at 4C.

Any other ideas as to what I should try in order to improve the resolution?

Thanks

Orly

Dr. Orly Dym
Israel Structural Proteomics Center
Department of Structural Biology
Weizmann Institute of Science

http://www.weizmann.ac.il/ISPC

972-8-934-3759 - Tel
972-8-934-4159 - Fax



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