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Looks like youre looking along a six-fold axis, perhaps serendipitous good stacking of your 2D plates? Could you index these? Flip -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Harry Powell Sent: Friday, 03 February, 2006 16:36 To: Tiago Barros Cc: [email protected] Subject: Re: [ccp4bb]: Curious diffraction pattern - non CCP4 related *** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** lovely. might it be due to an incommensurate modulated phase? > Dear all, > > I would like to share with you a very strange diffraction pattern that > I recently collected at the SLS. > > You can have a look at some pictures of this diffraction pattern here: > > http://tiagobarros.home.sapo.pt/zoom_in.png > > http://tiagobarros.home.sapo.pt/45.png (45 degrees) > > http://tiagobarros.home.sapo.pt/90.png > > http://tiagobarros.home.sapo.pt/135.png > > Some additional information. These happened with just one of my > crystals. I know from previous experience that my crystals (from a > membrane protein) are made of stacks of 2D crystals (SG P321). This > stacking is very often not that ordered, which leads to different > layers with different orientation within the same crystal. The space > group of my crystals is usually either C2 or P3121. > > I've found these circular motif of spots very curious, and I don't > really have a good explanation for them. > > Any idea of what might have caused this? > > Thank you. > > Tiago > > > ********************************************************************** > ** > * > > Tiago Barros > Max-Planck Institute of Biophysics > Dept. of Structural Biology > Max-von-Laue Str. 3 > D-60438 Frankfurt > Germany > > tel: +49-69-6303-3055 > > ********************************************************************** > ** > * > > > > > Harry -- Dr Harry Powell, MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, Cambridge, CB2 2QH
