Dear all, I have a structure at fairly low resolution that I am trying to refine with Refmac. I do not want to refine B factors so have arbitrarily set them all to 20 and then run refmac in the ccp4i GUI after deselecting the "refine temperature factors" box. However, when I look at the resulting pdb file my B factors vary from 2 to 90.
Is Refmac just calculating my B factors or is it still refining them, and if the latter how can I stop it? Thanks, Simon