NMR...take that! JPK
2011/9/5 Andreas Förster <[email protected]>: > AUC ! > > > Andreas > > > > On 05/09/2011 6:00, Jacob Keller wrote: >> >> mea culpa! How about FRET? >> >> JPK >> >> On Mon, Sep 5, 2011 at 11:39 AM, Bosch, Juergen<[email protected]> wrote: >>> >>> Hi Jacob, >>> you forgot cross-linking to stabilize a weak complex and verify that it >>> exists. >>> Jürgen >>> On Sep 5, 2011, at 11:45 AM, Jacob Keller wrote: >>> >>> Well, I guess I have always been curious what is the gold standard >>> here--perhaps SEC, ITC, SPR, pulldowns? What if SEC shows a >>> polydisperse sample with weak oligomerization, or SPR a very weak >>> binding constant? Do we then revert to a functional assay? Or what if >>> the functional assay does not show anything, but the binding constant >>> is really strong? Or vice versa, the binding is completely >>> undetectable, but the functional assay shows something? >>> >>> JPK >>> > > > -- > Andreas Förster, Research Associate > Paul Freemont & Xiaodong Zhang Labs > Department of Biochemistry, Imperial College London > http://www.msf.bio.ic.ac.uk > -- ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program cel: 773.608.9185 email: [email protected] *******************************************
