Hi Sunanda

They don't need to be "better" ... they need to be in agreement with you experimental B factor ... Wilson plot?


Best

Carlos

On 10/13/16 04:59, sunanda williams wrote:
Hi all,
I have a structure at 3.0 A and R/Rfree of 24/28. The mean B value is around 98. The B value is especially high at the N terminus and two loop regions (around 120-150 AA). The rest of the structure averaged around 70-75. Has anyone faced such a scenario? How reliable is the structure and is there any way to better the B factors.
Any help is appreciated.
Thank you!!


--
         Carlos CONTRERAS MARTEL, Ph.D.
                 (CR1 CNRS)
                
         carlos.contreras-mar...@ibs.fr
        
         "Bacterial Pathogenesis Group"
        Institut de Biologie Structurale
             UMR5075 CEA-CNRS-UGA

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