Dear CCP4BB,
Forgive me for soap boxing, but yesterday the first structure of a
GPCR/Gprotein complex was released (PDB:
http://www.rcsb.org/pdb/explore/explore.do?structureId=3SN6, article:
http://www.nature.com/nature/journal/vnfv/ncurrent/full/nature10361.html).
Recently a student
Hi Alex,
I read Filip's comment about volume not as a path length argument, but
about concentration uncertainty in mixing small volumes to dilute a
sample down before measuring it (?). I have never had to make a
dilution for my nanodrop (my proteins are usually not that
concentrated),
Hi Harvey,
Well, knowing nothing about your protein, allow me to ruminate anyway...
It sounds like you are exploring the possibility of a metal ion or
other cofactor being lost. This is a reasonable first thing to check,
but your buffer exchange steps should allow small cofactors (smaller
Dear Community,
In trying to trouble shoot an experiment I have become interested in
the cellular process that regulates the insertion and proper
orientation of membrane proteins. I am looking for references for how
a GPCR is correctly oriented during expression (i.e. the extra
cellular
Dear Community,
I am looking for a tool that can convert a potential MR search model
to match the residue number and a.a. type of my actual protein.
Specifically, I have a homolog structure, with slightly different
start and stop residues, and several non identical a.a.s relative to
my
Hi Liu,
If I understand your question correctly, youre asking how different
do two structures need to be for one to be new'. If by new you
mean a new fold, then the answer is NO. Your structure and the homolog
have the same fold.
However, if your structure is the first structure of a
Hi Sebastiano,
I have had some experience with protein:protein complexes with KD ~
10-1 uM, kinetic characterization and trying to purify a complex of
these proteins using SEC. While I would say that if you have reliable
evidence from SPR that you have a fast on (high Kon), then you must
Hi Francis,
I might save you some time by telling you up front you should just go
back and purify your compound to remove the impurity, you dont even
need to read the rest of this, just go.
Along the lines of what Savvas was saying, with any equilibrium
binding assay between two direct
Hi Ajit;
One of our CRT monitors broke recently, and in the context of
bemoaning the loss to a friend I was told that LCD monitors will not
work for stereo viewing. I understood the reason to be related to the
difference in refresh rates (?), with LCD's not being fast enough so
that the
Thanks to everyone for the info on Zalman monitors, sorry to have
muddied the waters for you Ajit. Best wishes~
~Justin
Quoting Justin Hall hallj...@onid.orst.edu:
Hi Ajit;
One of our CRT monitors broke recently, and in the context of
bemoaning the loss to a friend I was told that LCD
Dear All;
In response to my Anisotropic Diffraction In Refinement, which asked
for suggestions for how best to proceed with refinement with an
anisotropic data set, I received a large number of responses which
overwhelmingly suggested using the UCLA Anisotropy Server
?
Cheers~
~Justin Hall
Oregon State University
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