Hi Lisa, hi all,
Please do not discard the alternative method(s) of "conventional" heavy
atoms. Co-crystallisation or heavy-atom containing mother liquor soaks.
You may remember that "monster" complexes have been solved in the past
by such methods, and sometimes there are difficulties in cryst
On Tue, Jun 12, 2012 at 8:53 PM, Frank von Delft
wrote:
> Finding 111 sites should be feasible without other tricks than very careful
> data collection (see below); if you have two or more copies in the ASU, you
> may find you need to do what the ribosome guys did, namely use other
> derivatives
Hi Jun,
In this following article an metallic adapter was used to mount a 192 well
sitting drop plate on a conventional thermocycler.
http://journals.iucr.org/d/issues/2006/05/00/av5047/av5047bdy.html.
However I guess there would be significant evaporation and condensation if you
use vapor diff
If you don't have crystals yet, you'll find getting it to crystallize is
your main problem.
Finding 111 sites should be feasible without other tricks than very
careful data collection (see below); if you have two or more copies in
the ASU, you may find you need to do what the ribosome guys di
Why wouldn't a peltier-cooled/heated (PCR) thermal cycler be adaptable?
Roger Rowlett
On Jun 12, 2012 11:15 PM, "Jun Ma" wrote:
> Hi everybody,
>
> My experiment need me to try a thermo-cycling method for protein
> crystallization. So I need a kind of temperature chamber, which can changes
> tem
If you want to use se-Met, you might want to start by labeling only one protein
at a time. For example, if you have A,B,C,D, grow crystals like this:
se-A, B, C, D
A, se-B, C, D,
etc.
Then try combinations of 2, then 3, then if you haven't got the phases you
need, try all 4.
And remember, if
Hi everybody,
My experiment need me to try a thermo-cycling method for protein
crystallization. So I need a kind of temperature chamber, which can changes
temperature quickly, precisely and steadily. Furthermore, it should be
relatively quiet when running, because I'm afraid vibration will affect
Do you have crystals?
Do they diffract? If so, to what resolution?
What resolution do you require to answer your biological question?
F
On Jun 12, 2012, at 7:46 PM, LISA wrote:
> Hi all,
>
> My work is to solve huge complex containing 4 different proteins and total
> molecular weight is ab
I think there is a misconception floating around that processing your
data with "anomalous turned on" will somehow degrade the quality of
"normal" intensity data. I'm not exactly sure where this rumor comes
from, but I imagine it has something to do with confusion about all
the various "anomalous"
Hi all,
My work is to solve huge complex containing 4 different proteins and total
molecular weight is about 300 KD. I can purify the complex by co-expression
them in E.coli. This complex contains 8 protein A, 2 protein B and 1
protein C and D. protein B and protein C have homology structures
de
On Tuesday, June 12, 2012 02:29:13 pm Jerry McCully wrote:
>
> Dear ALL;
>
> After we solved our structure by anomalous scattering, we did a DALI
> search. Here are the results but it is not easy to draw meaningful
> conclusions whether our structure represents a novel fold or is homolo
...I meant visualisation software of course...
Quoting "VAN RAAIJ , MARK JOHAN":
if this is the first (or second, or third) time you do a DALI
search, take the list output from DALI, start from the top and
superpose each structure with yours and look at the superpositions
with your favouri
if this is the first (or second, or third) time you do a DALI search,
take the list output from DALI, start from the top and superpose each
structure with yours and look at the superpositions with your
favourite superposition software.
This is very educational and the only way you get a feeli
Dear ALL;
After we solved our structure by anomalous scattering, we did a DALI
search. Here are the results but it is not easy to draw meaningful conclusions
whether our structure represents a novel fold or is homologous to others.
Basically the Z-score is between 2 and 6.4 sinc
Why would anyone ignore the anomalous data they had collected? It will always
help the phasing, and decide the hand for you..
Eleanor
On 6 Jun 2012, at 03:55, Stefan Gajewski wrote:
> Hey!
>
> I was just wondering, do you know of any recent (~10y) publication that
> presented a structure sol
Dear all, this new vacancy at Diamond may be of interest for some readers of
this BB which is focused on further development of ISPyB at Diamond. Full
details of the post can be found here:
http://www.diamond.ac.uk/Home/Jobs/Current/DIA0737_TH.html
Informal inquiries can be made to myself (mart
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