[galaxy-dev] double check for EMS Variant Density Mapping

2014-04-22 Thread Wang, Xiaofei
Dear folks, Could you help me double check if my understanding is correct for EMS density mapping? For the tool CloudMap: EMS Variant Density Mapping, it does not work for other species except C.elegans, correct? For the workflow CloudMap EMS Variant Density Mapping workflow (takes VCF of

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-16 Thread Wang, Xiaofei
Dear folks, Why could not I find BDGP 5.74 in the drop-down menu of SnpEff? If I want to use this version, what should I do or what other steps do I need to install it? Thank you so much! From: Wang, Xiaofei Sent: Monday, April 14, 2014 9:50 AM To: Björn

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-14 Thread Wang, Xiaofei
still can not find it in the menu of SnpEff after downloading and installing it by the two-steps as below. Thanks a lot! Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent

[galaxy-dev] an error to SnpEff (CloudMap)

2014-04-14 Thread Wang, Xiaofei
Dear there, I got this error as below when I run CloudMap workflow in local galaxy instance. What is it the meaning of this error? Is it a general error or specific one for my data? Thanks a lot! Xiaofei error An error occurred with this dataset: WARNINGS: Some warning were detected Warning

Re: [galaxy-dev] how to delete datasets or save space on local galaxy

2014-04-14 Thread Wang, Xiaofei
...@gmail.com] Sent: Monday, April 14, 2014 8:47 AM To: Wang, Xiaofei Cc: Dannon Baker; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] how to delete datasets or save space on local galaxy Hello, That's a good question! Does the size in the top right change when the page is reloaded

[galaxy-dev] how to delete datasets or save space on local galaxy

2014-04-11 Thread Wang, Xiaofei
Dear there, May I ask a question which might be really simple? How to delete a dataset from a history in local Galaxy? When I clicked on delete (x), the size on disk is still the same as before deleting it. [cid:d28891d2-9d20-4688-8e3e-3bf75caa662e] Thank you so much! Best, Xiaofei

Re: [galaxy-dev] how to delete datasets or save space on local galaxy

2014-04-11 Thread Wang, Xiaofei
Baker [dannon.ba...@gmail.com] Sent: Friday, April 11, 2014 4:42 PM To: Wang, Xiaofei Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] how to delete datasets or save space on local galaxy Hi Xiaofei, The (x) simply flags a dataset for deletion, it does not remove it from disk. This happens

Re: [galaxy-dev] how to delete datasets or save space on local galaxy

2014-04-11 Thread Wang, Xiaofei
Yes, it works. But, I am still a little bit confuse about the size on the top right corner. Why does not it change after purging datasets? From: Dannon Baker [dannon.ba...@gmail.com] Sent: Friday, April 11, 2014 4:57 PM To: Wang, Xiaofei Cc: galaxy-dev

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-11 Thread Wang, Xiaofei
] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 3:52 PM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Oh, hooray, I figured it out. what I did is to go to this folder /Users/pablocingolani/snpEff and download

[galaxy-dev] Download workflow from main Galaxy

2014-04-04 Thread Wang, Xiaofei
Dear there, I tried to download workflow and import it into another my local Galaxy instance. But, when I downloaded and exported it from main Galaxy by Download to File, it showed as below. Not Found The resource could not be found. No route for

Re: [galaxy-dev] Download workflow from main Galaxy

2014-04-04 Thread Wang, Xiaofei
Yes, I really appreciate for your help! From: emulato...@gmail.com [emulato...@gmail.com] on behalf of Martin Čech [mar...@bx.psu.edu] Sent: Friday, April 04, 2014 11:28 AM To: Wang, Xiaofei Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] Download

Re: [galaxy-dev] version of SAM-to-BAM (CloudMap)

2014-04-04 Thread Wang, Xiaofei
Yes, it is figured out when I downloaded the version of 1.1.2. I thought is no longer available. Thank you so much! Best, Xiaofei From: Nate Coraor [n...@bx.psu.edu] Sent: Friday, April 04, 2014 11:20 AM To: Wang, Xiaofei Cc: galaxy-dev@lists.bx.psu.edu Subject

[galaxy-dev] Depth of Coverage (local galaxy __ CloudMap)

2014-04-04 Thread Wang, Xiaofei
Dear there, I got this error when I run CloudMap EMS Variant Density Mapping workflow for Depth of Coverage on the local Galaxy instance on Mac. In fact, it works fine for Depth of Coverage when I run another workflow (Unmapped Mutant workflow) locally. I really appreciate you guys for

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-03 Thread Wang, Xiaofei
From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Thursday, April 03, 2014 5:25 AM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Am 03.04.2014 04:34, schrieb Wang, Xiaofei: After SnpEff Databases Execute

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-03 Thread Wang, Xiaofei
Dear there, I am still stuck there for FASTQ Summary Statistics. Could you give me some clues to fix it? Thanks a lot! Best, Xiaofei From: Wang, Xiaofei Sent: Wednesday, April 02, 2014 11:21 AM To: Daniel Blankenberg Cc: Björn Grüning; galaxy-dev

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-03 Thread Wang, Xiaofei
[galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, April 03, 2014 10:31 AM To: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors 1. click SnpEff Download 2. choose one genome from SnpEff Databases

[galaxy-dev] version of SAM-to-BAM (CloudMap)

2014-04-03 Thread Wang, Xiaofei
Dear there, When I run CloudMap EMS Variant Density Mapping workflow (takes VCF of heterozygous and homozygous variants to subtract) (imported from uploaded file) on local Galaxy instance, I got this [X] , when I wanted to edit the workflow. Also, I got this The following tools are beinge

[galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-02 Thread Wang, Xiaofei
Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main java.lang.UnsupportedClassVersionError: ca/mcgill/mcb/pcingola/snpSift/SnpSift : Unsupported major.minor version 51.0 at java.lang.ClassLoader.defineClass1(Native Method) at

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-02 Thread Wang, Xiaofei
[bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear there, When I used SnpEff in local galaxy instance, I got an error: Exception in thread main

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-02 Thread Wang, Xiaofei
return self.nuc_index_base[ column ] IndexError: list index out of range From: Daniel Blankenberg [d...@bx.psu.edu] Sent: Wednesday, April 02, 2014 11:14 AM To: Wang, Xiaofei Cc: Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-02 Thread Wang, Xiaofei
option value=hg37Human (hg37)option option value=mm37.61Mouse (mm37.61)option param  From: Björn Grüning [bjoern.gruen...@gmail.com] Sent: Wednesday, April 02, 2014 10:35 AM To: Wang, Xiaofei; galaxy-dev

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-02 Thread Wang, Xiaofei
12:55 PM * To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu * Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors * * Hi, * * that means that the snpeff annotation is missing. You can install it * with the snpeff datamanager under your Admin panel

Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors

2014-04-02 Thread Wang, Xiaofei
: Wednesday, April 02, 2014 4:17 PM To: Wang, Xiaofei; Björn Grüning; galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] snpEff and FASTQ Summary Statistics errors Hi Xiaofei, Dear Bjoern, As your suggestion, I tried to install the snpEff annotation. But, I think I am still be stuck there. What I did

Re: [galaxy-dev] tool_dependency_dir (Installed, missing tool dependencies)

2014-03-13 Thread Wang, Xiaofei
[g...@bx.psu.edu] Sent: Tuesday, March 11, 2014 8:47 AM To: Wang, Xiaofei Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] tool_dependency_dir (Installed, missing tool dependencies) Hello, It's difficult to determine the cause of the problem in your environment based on the details

[galaxy-dev] tool_sheds_errors

2014-03-13 Thread Wang, Xiaofei
, March 11, 2014 8:47 AM To: Wang, Xiaofei Cc: galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] tool_dependency_dir (Installed, missing tool dependencies) Hello, It's difficult to determine the cause of the problem in your environment based on the details you've provided. If you have defined

Re: [galaxy-dev] tool_dependency_dir (Installed, missing tool dependencies)

2014-03-10 Thread Wang, Xiaofei
Hi folks, Could anybody answer my question and help me figure it out? Thank you so much! Best, Xiaofei From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Wang, Xiaofei [xfw...@ku.edu] Sent: Thursday, March 06, 2014 4

[galaxy-dev] tool_dependency_dir (Installed, missing tool dependencies)

2014-03-06 Thread Wang, Xiaofei
Dear there, I am trying to install some tools from toolshed on my local galaxy instance, like bwa_wrappers, package_picard_1_56_0, package_samtools_0_1_18, and sam_to_bam. For the first time, I got a warning before installing, something like this set the value of your tool_dependency_dir

Re: [galaxy-dev] Reference Genome in snpEff Tool

2014-02-17 Thread Wang, Xiaofei
I have the same question. But, I want to use it on Drosophila and use SnpEff for CloudMap pipeline. From: galaxy-dev-boun...@lists.bx.psu.edu [galaxy-dev-boun...@lists.bx.psu.edu] on behalf of Clint Christensen [cli...@txbiomedgenetics.org] Sent: Monday,

[galaxy-dev] SnpEff_and_CloudMap_for_other_speices_other_than_worm (galaxy not local)

2014-02-14 Thread Wang, Xiaofei
Dear, Is it possible to use SnpEff with genomes of other species (e.g. Drosophila) other than C.elegan on galaxy website (not local)? It seems the only option for the genome menu (Caenorhabditis elegants : WS220.64). In fact, I am trying to use CloudMap (the EMS Variant Density Mapping

Re: [galaxy-dev] SnpEff_and_CloudMap_for_other_speices_other_than_worm (galaxy not local)

2014-02-14 Thread Wang, Xiaofei
, GATK for calling SNPs, ...) by hand not use the CloudMap pipeline on Drosophila? Thanks From: Wolfgang Maier [wolfgang.ma...@biologie.uni-freiburg.de] Sent: Friday, February 14, 2014 11:48 AM To: Wang, Xiaofei Cc: galaxy-dev@lists.bx.psu.edu Subject: RE: [galaxy-dev